ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY
In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow. Bacterial cells were hybridized with 16S rRNA targeted oligonucleotide probes specific for the ph...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-12-01
|
| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/17079 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow. Bacterial cells were hybridized with 16S rRNA targeted oligonucleotide probes specific for the phylogenetic group Cytophaga-Flexibacter-Bacteroides (CFB) (BACPRE) and for species Prevotella bryantii B14 (PBB14) and P.ruminicola 23T (PR23). The oligonucleotide probes were labelled with fluorescine isothiocyanate (FITC) or tetramethylrodamine isothiocyanate (TRITC). Both species specific probes proved to be highly specific and gave strong and clear signal. |
|---|---|
| ISSN: | 1854-1941 |