Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout
Gout is a common metabolic disease in humans, and it is due to persistently elevated levels of uric acid in the blood. At high levels, uric acid crystallizes and the crystals deposit in joints and surrounding tissues, resulting in an attack of gout. Interestingly, the gout attack can spontaneously r...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/7143241 |
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| author | Lihua Duan Jiao Luo Qiang Fu Ke Shang Yingying Wei Youlian Wang Yan Li Jie Chen |
| author_facet | Lihua Duan Jiao Luo Qiang Fu Ke Shang Yingying Wei Youlian Wang Yan Li Jie Chen |
| author_sort | Lihua Duan |
| collection | DOAJ |
| description | Gout is a common metabolic disease in humans, and it is due to persistently elevated levels of uric acid in the blood. At high levels, uric acid crystallizes and the crystals deposit in joints and surrounding tissues, resulting in an attack of gout. Interestingly, the gout attack can spontaneously resolve within a few days. However, the self-limited mechanism of gout remains elusive. It has been demonstrated that CD14 plays an important role in self-remission of gout. In this study, we found that the proportion of CD14-positive PBMCs was decreased in gout patients when compared with healthy controls and the serum sCD14 level was also considerably decreased in gout patients in comparison to healthy controls. In addition, sCD14 levels were positively correlated with CRP levels. Furthermore, the effect of MSU on the levels of CD14 in healthy volunteer’s PBMC was explored in in vitro experiment. The results showed that CD14 expression on macrophage and sCD14 levels in the culture supernatants were significantly decreased after MSU treatment. However, there was no significance in the levels of membrane CD14 and sCD14 in healthy volunteer’s PBMC stimulated by LPS. Taken together, these results suggest that CD14 might play an important role in self-remission of gout. |
| format | Article |
| id | doaj-art-56c0dfb8affd428e9a933bf0eef900d2 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-56c0dfb8affd428e9a933bf0eef900d22025-02-03T01:25:23ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/71432417143241Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute GoutLihua Duan0Jiao Luo1Qiang Fu2Ke Shang3Yingying Wei4Youlian Wang5Yan Li6Jie Chen7Department of Rheumatology and Clinical Immunology, Jiangxi Provincial People’s Hospital, Nanchang, ChinaDepartment of Rheumatology and Clinical Immunology, University of Lübeck, GermanyDepartment of Rheumatology and Clinical Immunology, Jiangxi Provincial People’s Hospital, Nanchang, ChinaDepartment of Rheumatology and Clinical Immunology, Jiangxi Provincial People’s Hospital, Nanchang, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, Jiangxi Provincial People’s Hospital, Nanchang, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, Xiamen, ChinaDepartment of Scientific Research and Education, Jiangxi Provincial People’s Hospital, Nanchang, ChinaGout is a common metabolic disease in humans, and it is due to persistently elevated levels of uric acid in the blood. At high levels, uric acid crystallizes and the crystals deposit in joints and surrounding tissues, resulting in an attack of gout. Interestingly, the gout attack can spontaneously resolve within a few days. However, the self-limited mechanism of gout remains elusive. It has been demonstrated that CD14 plays an important role in self-remission of gout. In this study, we found that the proportion of CD14-positive PBMCs was decreased in gout patients when compared with healthy controls and the serum sCD14 level was also considerably decreased in gout patients in comparison to healthy controls. In addition, sCD14 levels were positively correlated with CRP levels. Furthermore, the effect of MSU on the levels of CD14 in healthy volunteer’s PBMC was explored in in vitro experiment. The results showed that CD14 expression on macrophage and sCD14 levels in the culture supernatants were significantly decreased after MSU treatment. However, there was no significance in the levels of membrane CD14 and sCD14 in healthy volunteer’s PBMC stimulated by LPS. Taken together, these results suggest that CD14 might play an important role in self-remission of gout.http://dx.doi.org/10.1155/2019/7143241 |
| spellingShingle | Lihua Duan Jiao Luo Qiang Fu Ke Shang Yingying Wei Youlian Wang Yan Li Jie Chen Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout Journal of Immunology Research |
| title | Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout |
| title_full | Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout |
| title_fullStr | Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout |
| title_full_unstemmed | Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout |
| title_short | Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout |
| title_sort | decreased expression of cd14 in msu mediated inflammation may be associated with spontaneous remission of acute gout |
| url | http://dx.doi.org/10.1155/2019/7143241 |
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