Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study.
<h4>Aims</h4>Endogenous cardiac progenitor cells, expanded from explants via cardiosphere formation, present a promising cell source to prevent heart failure following myocardial infarction. Here we used cine-magnetic resonance imaging (MRI) to track administered cardiosphere-derived cel...
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025669&type=printable |
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| author | Carolyn A Carr Daniel J Stuckey Jun Jie Tan Suat Cheng Tan Renata S M Gomes Patrizia Camelliti Elisa Messina Alessandro Giacomello Georgina M Ellison Kieran Clarke |
| author_facet | Carolyn A Carr Daniel J Stuckey Jun Jie Tan Suat Cheng Tan Renata S M Gomes Patrizia Camelliti Elisa Messina Alessandro Giacomello Georgina M Ellison Kieran Clarke |
| author_sort | Carolyn A Carr |
| collection | DOAJ |
| description | <h4>Aims</h4>Endogenous cardiac progenitor cells, expanded from explants via cardiosphere formation, present a promising cell source to prevent heart failure following myocardial infarction. Here we used cine-magnetic resonance imaging (MRI) to track administered cardiosphere-derived cells (CDCs) and to measure changes in cardiac function over four months in the infarcted rat heart.<h4>Methods and results</h4>CDCs, cultured from neonatal rat heart, comprised a heterogeneous population including cells expressing the mesenchymal markers CD90 and CD105, the stem cell marker c-kit and the pluripotency markers Sox2, Oct3/4 and Klf-4. CDCs (2 × 10(6)) expressing green fluorescent protein (GFP+) were labelled with fluorescent micron-sized particles of iron oxide (MPIO). Labelled cells were administered to the infarcted rat hearts (n = 7) by intramyocardial injection immediately following reperfusion, then by systemic infusion (4 × 10(6)) 2 days later. A control group (n = 7) was administered cell medium. MR hypointensities caused by the MPIOs were detected at all times and GFP+ cells containing MPIO particles were identified in tissue slices at 16 weeks. At two days after infarction, cardiac function was similar between groups. By 6 weeks, ejection fractions in control hearts had significantly decreased (47 ± 2%), but this was not evident in CDC-treated hearts (56 ± 3%). The significantly higher ejection fractions in the CDC-treated group were maintained for a further 10 weeks. In addition, CDC-treated rat hearts had significantly increased capillary density in the peri-infarct region and lower infarct sizes. MPIO-labelled cells also expressed cardiac troponin I, von Willebrand factor and smooth muscle actin, suggesting their differentiation along the cardiomyocyte lineage and the formation of new blood vessels.<h4>Conclusions</h4>CDCs were retained in the infarcted rat heart for 16 weeks and improved cardiac function. |
| format | Article |
| id | doaj-art-56bec4e84de944968532ecdbdb24dbc2 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-56bec4e84de944968532ecdbdb24dbc22025-08-20T02:05:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2566910.1371/journal.pone.0025669Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study.Carolyn A CarrDaniel J StuckeyJun Jie TanSuat Cheng TanRenata S M GomesPatrizia CamellitiElisa MessinaAlessandro GiacomelloGeorgina M EllisonKieran Clarke<h4>Aims</h4>Endogenous cardiac progenitor cells, expanded from explants via cardiosphere formation, present a promising cell source to prevent heart failure following myocardial infarction. Here we used cine-magnetic resonance imaging (MRI) to track administered cardiosphere-derived cells (CDCs) and to measure changes in cardiac function over four months in the infarcted rat heart.<h4>Methods and results</h4>CDCs, cultured from neonatal rat heart, comprised a heterogeneous population including cells expressing the mesenchymal markers CD90 and CD105, the stem cell marker c-kit and the pluripotency markers Sox2, Oct3/4 and Klf-4. CDCs (2 × 10(6)) expressing green fluorescent protein (GFP+) were labelled with fluorescent micron-sized particles of iron oxide (MPIO). Labelled cells were administered to the infarcted rat hearts (n = 7) by intramyocardial injection immediately following reperfusion, then by systemic infusion (4 × 10(6)) 2 days later. A control group (n = 7) was administered cell medium. MR hypointensities caused by the MPIOs were detected at all times and GFP+ cells containing MPIO particles were identified in tissue slices at 16 weeks. At two days after infarction, cardiac function was similar between groups. By 6 weeks, ejection fractions in control hearts had significantly decreased (47 ± 2%), but this was not evident in CDC-treated hearts (56 ± 3%). The significantly higher ejection fractions in the CDC-treated group were maintained for a further 10 weeks. In addition, CDC-treated rat hearts had significantly increased capillary density in the peri-infarct region and lower infarct sizes. MPIO-labelled cells also expressed cardiac troponin I, von Willebrand factor and smooth muscle actin, suggesting their differentiation along the cardiomyocyte lineage and the formation of new blood vessels.<h4>Conclusions</h4>CDCs were retained in the infarcted rat heart for 16 weeks and improved cardiac function.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025669&type=printable |
| spellingShingle | Carolyn A Carr Daniel J Stuckey Jun Jie Tan Suat Cheng Tan Renata S M Gomes Patrizia Camelliti Elisa Messina Alessandro Giacomello Georgina M Ellison Kieran Clarke Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. PLoS ONE |
| title | Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. |
| title_full | Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. |
| title_fullStr | Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. |
| title_full_unstemmed | Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. |
| title_short | Cardiosphere-derived cells improve function in the infarcted rat heart for at least 16 weeks--an MRI study. |
| title_sort | cardiosphere derived cells improve function in the infarcted rat heart for at least 16 weeks an mri study |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025669&type=printable |
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