Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis
Species identification of <i>Listeria</i> isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus <i>Listeria</i>. It is advisable to provide new precise techni...
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2024-10-01
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| author | Antonio Martínez-Murcia Aaron Navarro Caridad Miró-Pina |
| author_facet | Antonio Martínez-Murcia Aaron Navarro Caridad Miró-Pina |
| author_sort | Antonio Martínez-Murcia |
| collection | DOAJ |
| description | Species identification of <i>Listeria</i> isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus <i>Listeria</i>. It is advisable to provide new precise techniques since this taxon includes two pathogens that are usually transmitted through the food chain, <i>Listeria monocytogenes</i> and <i>L. ivanovii</i>. The approach, so-called multilocus phylogenetic analysis (MLPA) that uses several concatenated housekeeping gene sequences, provides accurate and affordable classification frameworks to easily identify <i>Listeria</i> species by simple Sanger sequencing. Fragments of seven housekeeping genes (<i>gyrA</i>, <i>cpn60</i>, <i>parE</i>, <i>recA</i>, <i>rpoB</i>, <i>atpA</i>, and <i>gyrB</i>) from 218 strains of all <i>Listeria</i> species currently described were used to build an MLPA of the concatenated sequence, a total of 4375 bp. All isolates subjected to identification were clustered within the species of <i>Listeria</i> sensu stricto, <i>L. monocytogenes</i>, <i>L. innocua</i>, and <i>L. welshimeri</i>, and some reference strains were reclassified as <i>L. ivanovii</i> and <i>L. seeligeri</i>. Housekeeping-gene sequencing has been demonstrated to represent a pragmatic tool that can be firmly considered in food control. |
| format | Article |
| id | doaj-art-56b4933142d540449dbcec8ea9e0e872 |
| institution | DOAJ |
| issn | 2036-7481 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Microbiology Research |
| spelling | doaj-art-56b4933142d540449dbcec8ea9e0e8722025-08-20T02:56:56ZengMDPI AGMicrobiology Research2036-74812024-10-011542114212810.3390/microbiolres15040142Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic AnalysisAntonio Martínez-Murcia0Aaron Navarro1Caridad Miró-Pina2Department of Microbiology, University Miguel Hernández, Orihuela, 03312 Alicante, SpainGenetic PCR Solutions™, Orihuela, 03300 Alicante, SpainGenetic PCR Solutions™, Orihuela, 03300 Alicante, SpainSpecies identification of <i>Listeria</i> isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus <i>Listeria</i>. It is advisable to provide new precise techniques since this taxon includes two pathogens that are usually transmitted through the food chain, <i>Listeria monocytogenes</i> and <i>L. ivanovii</i>. The approach, so-called multilocus phylogenetic analysis (MLPA) that uses several concatenated housekeeping gene sequences, provides accurate and affordable classification frameworks to easily identify <i>Listeria</i> species by simple Sanger sequencing. Fragments of seven housekeeping genes (<i>gyrA</i>, <i>cpn60</i>, <i>parE</i>, <i>recA</i>, <i>rpoB</i>, <i>atpA</i>, and <i>gyrB</i>) from 218 strains of all <i>Listeria</i> species currently described were used to build an MLPA of the concatenated sequence, a total of 4375 bp. All isolates subjected to identification were clustered within the species of <i>Listeria</i> sensu stricto, <i>L. monocytogenes</i>, <i>L. innocua</i>, and <i>L. welshimeri</i>, and some reference strains were reclassified as <i>L. ivanovii</i> and <i>L. seeligeri</i>. Housekeeping-gene sequencing has been demonstrated to represent a pragmatic tool that can be firmly considered in food control.https://www.mdpi.com/2036-7481/15/4/142<i>Listeria</i><i>L. monocytogenes</i>multilocus phylogenetic analysisMLPA |
| spellingShingle | Antonio Martínez-Murcia Aaron Navarro Caridad Miró-Pina Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis Microbiology Research <i>Listeria</i> <i>L. monocytogenes</i> multilocus phylogenetic analysis MLPA |
| title | Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis |
| title_full | Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis |
| title_fullStr | Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis |
| title_full_unstemmed | Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis |
| title_short | Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis |
| title_sort | identification of i listeria i isolates by using a pragmatic multilocus phylogenetic analysis |
| topic | <i>Listeria</i> <i>L. monocytogenes</i> multilocus phylogenetic analysis MLPA |
| url | https://www.mdpi.com/2036-7481/15/4/142 |
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