Identification of <i>Listeria</i> Isolates by Using a Pragmatic Multilocus Phylogenetic Analysis
Species identification of <i>Listeria</i> isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus <i>Listeria</i>. It is advisable to provide new precise techni...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/15/4/142 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Species identification of <i>Listeria</i> isolates remained a tedious process still based on culturing methods that, in recent years, have led to the description of many species that are not even part of the genus <i>Listeria</i>. It is advisable to provide new precise techniques since this taxon includes two pathogens that are usually transmitted through the food chain, <i>Listeria monocytogenes</i> and <i>L. ivanovii</i>. The approach, so-called multilocus phylogenetic analysis (MLPA) that uses several concatenated housekeeping gene sequences, provides accurate and affordable classification frameworks to easily identify <i>Listeria</i> species by simple Sanger sequencing. Fragments of seven housekeeping genes (<i>gyrA</i>, <i>cpn60</i>, <i>parE</i>, <i>recA</i>, <i>rpoB</i>, <i>atpA</i>, and <i>gyrB</i>) from 218 strains of all <i>Listeria</i> species currently described were used to build an MLPA of the concatenated sequence, a total of 4375 bp. All isolates subjected to identification were clustered within the species of <i>Listeria</i> sensu stricto, <i>L. monocytogenes</i>, <i>L. innocua</i>, and <i>L. welshimeri</i>, and some reference strains were reclassified as <i>L. ivanovii</i> and <i>L. seeligeri</i>. Housekeeping-gene sequencing has been demonstrated to represent a pragmatic tool that can be firmly considered in food control. |
|---|---|
| ISSN: | 2036-7481 |