Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)

Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supported by the studies that moss contains antioxidant, antimicrobial and antitumoral compounds. It was aimed to determine the immunoreactivity of Cu / Zn SOD enzyme in the kidney, adrenal gland, and ovari...

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Main Authors: Özlem Yayıntaş, Latife Ceyda İrkin, Şamil Öztürk
Format: Article
Language:English
Published: Prensip Publishing 2021-10-01
Series:Acta Natura et Scientia
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Online Access:https://prensipjournals.com/ojs/index.php/actanatsci/article/view/192
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author Özlem Yayıntaş
Latife Ceyda İrkin
Şamil Öztürk
author_facet Özlem Yayıntaş
Latife Ceyda İrkin
Şamil Öztürk
author_sort Özlem Yayıntaş
collection DOAJ
description Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supported by the studies that moss contains antioxidant, antimicrobial and antitumoral compounds. It was aimed to determine the immunoreactivity of Cu / Zn SOD enzyme in the kidney, adrenal gland, and ovarian tissues of rats due to the increase in the dose of moss extract. In this study, 1 mL of distilled water were given control groups (G1), 50 mg/kg (G2), 100 mg/kg (G3), 300 mg/kg (G4) and 500 mg/kg (G5) moss extract were administered by gavage for 30 days another groups. At the end of the experiment period, the tissues taken from the rats were subjected to routine histopathological procedures. Cu /Z n SOD primary antibody was applied using immunohistochemical staining methods to detect immunoreactivity. The study was terminated by using the Kruskal-Wallis test, which is one of the nonparametric tests. To determine the differences between the groups by evaluating the stained tissue samples with the image analysis system in the light microscope. A significant difference was found in the dose-related positivity of the kidney, ovarian and adrenal gland tissues of the groups given moss extract p. It has been determined that H. sericeum species increases Cu/Zn SOD enzyme activity in the kidney, adrenal gland and ovarian tissues, and its cytotoxic effects shows a dose-related increase in the histopathological table.
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spelling doaj-art-56b45bb9cab14d5e8008cd8c293ef9212025-08-20T02:30:47ZengPrensip PublishingActa Natura et Scientia2718-06382021-10-012210.29329/actanatsci.2021.350.04192Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)Özlem Yayıntaş0https://orcid.org/0000-0002-3554-1881Latife Ceyda İrkin1https://orcid.org/0000-0001-6603-8413Şamil Öztürk2https://orcid.org/0000-0002-9435-8139Çanakkale Onsekiz Mart University, Faculty of Medicine, Department of Medical Biology, 17020 Çanakkale, TurkeyÇanakkale Onsekiz Mart University, Çanakkale Faculty of Applied Sciences, Department of Fisheries Technology, 17020 Çanakkale, TurkeyÇanakkale Onsekiz Mart University, Vocational School of Health Services, 17020 Çanakkale, Turkey Homalothecium sericeum (Hedw.) Schimp. is growing in habitats such as walls and roofs. It is supported by the studies that moss contains antioxidant, antimicrobial and antitumoral compounds. It was aimed to determine the immunoreactivity of Cu / Zn SOD enzyme in the kidney, adrenal gland, and ovarian tissues of rats due to the increase in the dose of moss extract. In this study, 1 mL of distilled water were given control groups (G1), 50 mg/kg (G2), 100 mg/kg (G3), 300 mg/kg (G4) and 500 mg/kg (G5) moss extract were administered by gavage for 30 days another groups. At the end of the experiment period, the tissues taken from the rats were subjected to routine histopathological procedures. Cu /Z n SOD primary antibody was applied using immunohistochemical staining methods to detect immunoreactivity. The study was terminated by using the Kruskal-Wallis test, which is one of the nonparametric tests. To determine the differences between the groups by evaluating the stained tissue samples with the image analysis system in the light microscope. A significant difference was found in the dose-related positivity of the kidney, ovarian and adrenal gland tissues of the groups given moss extract p. It has been determined that H. sericeum species increases Cu/Zn SOD enzyme activity in the kidney, adrenal gland and ovarian tissues, and its cytotoxic effects shows a dose-related increase in the histopathological table. https://prensipjournals.com/ojs/index.php/actanatsci/article/view/192Cu/Zn SOD (SOD1)KidneyAdrenal glandOvaryMossImmunohistochemistry
spellingShingle Özlem Yayıntaş
Latife Ceyda İrkin
Şamil Öztürk
Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)
Acta Natura et Scientia
Cu/Zn SOD (SOD1)
Kidney
Adrenal gland
Ovary
Moss
Immunohistochemistry
title Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)
title_full Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)
title_fullStr Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)
title_full_unstemmed Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)
title_short Effect of Homalothecium sericeum (Hedw.) Schimp. Extract on SOD1 Activity in Rat Tissues (Kidney, Adrenal Gland, Ovary)
title_sort effect of homalothecium sericeum hedw schimp extract on sod1 activity in rat tissues kidney adrenal gland ovary
topic Cu/Zn SOD (SOD1)
Kidney
Adrenal gland
Ovary
Moss
Immunohistochemistry
url https://prensipjournals.com/ojs/index.php/actanatsci/article/view/192
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