Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study

Background:Working memory (WM) is a core cognitive function closely linked to various cognitive processes including language, decision making, and reasoning. Transcranial alternating current stimulation (tACS), a non-invasive brain stimulation technique, has been shown to modulate cognitive abilitie...

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Main Authors: Binbin Gao, Jinyan Zhang, Jianxu Zhang, Guangying Pei, Tiantian Liu, Li Wang, Shintaro Funahashi, Jinglong Wu, Zhilin Zhang, Jian Zhang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Brain Sciences
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Online Access:https://www.mdpi.com/2076-3425/15/4/381
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author Binbin Gao
Jinyan Zhang
Jianxu Zhang
Guangying Pei
Tiantian Liu
Li Wang
Shintaro Funahashi
Jinglong Wu
Zhilin Zhang
Jian Zhang
author_facet Binbin Gao
Jinyan Zhang
Jianxu Zhang
Guangying Pei
Tiantian Liu
Li Wang
Shintaro Funahashi
Jinglong Wu
Zhilin Zhang
Jian Zhang
author_sort Binbin Gao
collection DOAJ
description Background:Working memory (WM) is a core cognitive function closely linked to various cognitive processes including language, decision making, and reasoning. Transcranial alternating current stimulation (tACS), a non-invasive brain stimulation technique, has been shown to modulate cognitive abilities and treat psychiatric disorders. Although gamma tACS (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS) has demonstrated positive effects on WM, its underlying neural mechanisms remain unclear. Methods: In this study, we employed electroencephalogram (EEG) microstate analysis to investigate the spatiotemporal dynamics of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS effects on WM performance. Healthy participants (N = 104) participated in two-back and three-back WM tasks before and after two types (sine and triangular) of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS, with sham stimulation as a control. Results: Our results revealed that <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS improved performance in both the two-back and three-back tasks, with triangular <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS showing greater accuracy improvement in the three-back task than the sham group. Furthermore, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS significantly modulated EEG microstate dynamics, specifically downregulating microstate Class C and upregulating microstate Classes D and B. These changes were positively correlated with reduced reaction times in the three-back task. Conclusions: Our findings establish microstate analysis as an effective approach for evaluating <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS-induced changes in global brain activity and advance the understanding of how <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS influences WM.
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spelling doaj-art-56aed81caab747dc8d860d6f3dba38cc2025-08-20T03:14:23ZengMDPI AGBrain Sciences2076-34252025-04-0115438110.3390/brainsci15040381Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate StudyBinbin Gao0Jinyan Zhang1Jianxu Zhang2Guangying Pei3Tiantian Liu4Li Wang5Shintaro Funahashi6Jinglong Wu7Zhilin Zhang8Jian Zhang9School of Life Science, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Medical Technology, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Medical Technology, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Medical Technology, Beijing Institute of Technology, Beijing 100081, ChinaAdvanced Research Institute for Multidisciplinary Science, Beijing Institute of Technology, Beijing 100081, ChinaSchool of Medical Technology, Beijing Institute of Technology, Beijing 100081, ChinaResearch Center for Medical Artificial Intelligence, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaSchool of Medical Technology, Beijing Institute of Technology, Beijing 100081, ChinaBackground:Working memory (WM) is a core cognitive function closely linked to various cognitive processes including language, decision making, and reasoning. Transcranial alternating current stimulation (tACS), a non-invasive brain stimulation technique, has been shown to modulate cognitive abilities and treat psychiatric disorders. Although gamma tACS (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS) has demonstrated positive effects on WM, its underlying neural mechanisms remain unclear. Methods: In this study, we employed electroencephalogram (EEG) microstate analysis to investigate the spatiotemporal dynamics of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS effects on WM performance. Healthy participants (N = 104) participated in two-back and three-back WM tasks before and after two types (sine and triangular) of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS, with sham stimulation as a control. Results: Our results revealed that <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS improved performance in both the two-back and three-back tasks, with triangular <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS showing greater accuracy improvement in the three-back task than the sham group. Furthermore, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS significantly modulated EEG microstate dynamics, specifically downregulating microstate Class C and upregulating microstate Classes D and B. These changes were positively correlated with reduced reaction times in the three-back task. Conclusions: Our findings establish microstate analysis as an effective approach for evaluating <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS-induced changes in global brain activity and advance the understanding of how <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>γ</mi></semantics></math></inline-formula>-tACS influences WM.https://www.mdpi.com/2076-3425/15/4/381γ-tACSworking memorymicrostateresting-state EEG
spellingShingle Binbin Gao
Jinyan Zhang
Jianxu Zhang
Guangying Pei
Tiantian Liu
Li Wang
Shintaro Funahashi
Jinglong Wu
Zhilin Zhang
Jian Zhang
Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study
Brain Sciences
γ-tACS
working memory
microstate
resting-state EEG
title Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study
title_full Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study
title_fullStr Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study
title_full_unstemmed Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study
title_short Gamma Transcranial Alternating Current Stimulation Enhances Working Memory Ability in Healthy People: An EEG Microstate Study
title_sort gamma transcranial alternating current stimulation enhances working memory ability in healthy people an eeg microstate study
topic γ-tACS
working memory
microstate
resting-state EEG
url https://www.mdpi.com/2076-3425/15/4/381
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