In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents
Abstract Microbial exopolysaccharides (EPSs) possess valuable biological functions and fascinating physicochemical properties. On the other hand, lung cancer is the primary contributor to global cancer-related deaths. However, health and safety concerns have prevented the identification and approval...
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BMC
2024-12-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-024-02601-z |
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| author | Nourhan S. Shehata Bassma H. Elwakil Salma S. Elshewemi Doaa A. Ghareeb Zakia A. Olama |
| author_facet | Nourhan S. Shehata Bassma H. Elwakil Salma S. Elshewemi Doaa A. Ghareeb Zakia A. Olama |
| author_sort | Nourhan S. Shehata |
| collection | DOAJ |
| description | Abstract Microbial exopolysaccharides (EPSs) possess valuable biological functions and fascinating physicochemical properties. On the other hand, lung cancer is the primary contributor to global cancer-related deaths. However, health and safety concerns have prevented the identification and approval of any medications, including chemotherapeutic agents, for lung cancer treatment to date. The current study aims to enhance the production of bacterial EPS as a coating agent for the synthesis of selenium nanoparticles (AZEPS-SeNPs), to enhance their biological activity against pathogenic microbes, human lung adenocarcinoma cells (A549) in vitro, and diethyl nitrosamine (DEN)-induced lung cancer in vivo. The synthesized AZEPS-SeNPs exhibited a significant antifungal effect reaching 49.3 mm against Candida albicans. SeNPs and EPSs demonstrated a concentration-dependent synergistic antioxidant effect of 96.8%. Moreover, the synthesized nanoparticles showed a highly potent cytotoxic effect against A549 cells (1.724 ± 0.08 µg/mL) with a therapeutic index of 7.18 ± 0.21 that leads to increased reactive oxygen species (ROS) production. AZEPS-SeNPs demonstrated a proapoptotic effect on the lung adenocarcinoma A549 cell line by stimulating caspase 3 and Bax (7.08-fold and 6.505-fold, respectively), inhibiting the anti-apoptotic gene Bcl2, and arresting the cell cycle in the S phase. In vivo study revealed that the AZEPS-SeNPs-treated group showed improved histopathological examination of lung tissue sections. The present study concluded the efficiency of the synthesized bacterial EPS-SeNPs as multi-functional antimicrobial, anticancer and antioxidant agent. |
| format | Article |
| id | doaj-art-56ae61f5359c4d098a655bdc5d58f4a5 |
| institution | OA Journals |
| issn | 1475-2859 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-56ae61f5359c4d098a655bdc5d58f4a52025-08-20T01:59:47ZengBMCMicrobial Cell Factories1475-28592024-12-0123112010.1186/s12934-024-02601-zIn vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agentsNourhan S. Shehata0Bassma H. Elwakil1Salma S. Elshewemi2Doaa A. Ghareeb3Zakia A. Olama4Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in AlexandriaDepartment of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in AlexandriaZoology Department, Faculty of Science, Alexandria UniversityBio-Screening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria UniversityDepartment of Botany and Microbiology, Faculty of Science, Alexandria UniversityAbstract Microbial exopolysaccharides (EPSs) possess valuable biological functions and fascinating physicochemical properties. On the other hand, lung cancer is the primary contributor to global cancer-related deaths. However, health and safety concerns have prevented the identification and approval of any medications, including chemotherapeutic agents, for lung cancer treatment to date. The current study aims to enhance the production of bacterial EPS as a coating agent for the synthesis of selenium nanoparticles (AZEPS-SeNPs), to enhance their biological activity against pathogenic microbes, human lung adenocarcinoma cells (A549) in vitro, and diethyl nitrosamine (DEN)-induced lung cancer in vivo. The synthesized AZEPS-SeNPs exhibited a significant antifungal effect reaching 49.3 mm against Candida albicans. SeNPs and EPSs demonstrated a concentration-dependent synergistic antioxidant effect of 96.8%. Moreover, the synthesized nanoparticles showed a highly potent cytotoxic effect against A549 cells (1.724 ± 0.08 µg/mL) with a therapeutic index of 7.18 ± 0.21 that leads to increased reactive oxygen species (ROS) production. AZEPS-SeNPs demonstrated a proapoptotic effect on the lung adenocarcinoma A549 cell line by stimulating caspase 3 and Bax (7.08-fold and 6.505-fold, respectively), inhibiting the anti-apoptotic gene Bcl2, and arresting the cell cycle in the S phase. In vivo study revealed that the AZEPS-SeNPs-treated group showed improved histopathological examination of lung tissue sections. The present study concluded the efficiency of the synthesized bacterial EPS-SeNPs as multi-functional antimicrobial, anticancer and antioxidant agent.https://doi.org/10.1186/s12934-024-02601-zExopolysaccharideAntimicrobialAnticancerOptimizationSelenium nanoparticles coated bacterial polysaccharide |
| spellingShingle | Nourhan S. Shehata Bassma H. Elwakil Salma S. Elshewemi Doaa A. Ghareeb Zakia A. Olama In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents Microbial Cell Factories Exopolysaccharide Antimicrobial Anticancer Optimization Selenium nanoparticles coated bacterial polysaccharide |
| title | In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents |
| title_full | In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents |
| title_fullStr | In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents |
| title_full_unstemmed | In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents |
| title_short | In vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti-lung cancer agents |
| title_sort | in vitro and in vivo studies of selenium nanoparticles coated bacterial polysaccharide as anti lung cancer agents |
| topic | Exopolysaccharide Antimicrobial Anticancer Optimization Selenium nanoparticles coated bacterial polysaccharide |
| url | https://doi.org/10.1186/s12934-024-02601-z |
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