Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing
Fruiting body formation in edible fungi is a critical development process for both scientific understanding and industrial cultivation, yet the underlying genetic mechanisms remain poorly elucidated. This study aimed to identify key genes regulating monokaryotic fruiting in <i>Flammulina filif...
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MDPI AG
2025-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/11/7/512 |
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| author | Peng Wang Ya Yu Lei Xia Qi Yan Xiao Tan Dongyin Wang Xue Wang Zhibin Zhang Jiawei Wen Xiao Huang |
| author_facet | Peng Wang Ya Yu Lei Xia Qi Yan Xiao Tan Dongyin Wang Xue Wang Zhibin Zhang Jiawei Wen Xiao Huang |
| author_sort | Peng Wang |
| collection | DOAJ |
| description | Fruiting body formation in edible fungi is a critical development process for both scientific understanding and industrial cultivation, yet the underlying genetic mechanisms remain poorly elucidated. This study aimed to identify key genes regulating monokaryotic fruiting in <i>Flammulina filiformis</i>. A genetic segregation population was constructed through selfing purification and hybrid segregation of the FF002 strain, followed by mapping candidate genes with bulked segregant analysis sequencing (BSA-seq). A 10 kb genomic region on scaffold19 was identified, pinpointing the gene <i>FV-L110034160</i>, which encodes a U2 snRNP complex component involved in pre-mRNA splicing. A T→G SNP located 121 bp downstream of the ATG codon caused a serine-to-alanine substitution, disrupting a conserved domain and altering fruiting phenotypes. Phylogenetic analysis further revealed conservation of this gene in fungal genera. These findings elucidate a key regulatory gene controlling monokaryotic fruiting in <i>F. filiformis</i>, providing novel insights into fruiting body formation mechanisms and establishing a foundation for genetic studies in other edible fungi. |
| format | Article |
| id | doaj-art-56a60763f821499e8b56f53efbc81bbc |
| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-56a60763f821499e8b56f53efbc81bbc2025-08-20T02:45:37ZengMDPI AGJournal of Fungi2309-608X2025-07-0111751210.3390/jof11070512Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis SequencingPeng Wang0Ya Yu1Lei Xia2Qi Yan3Xiao Tan4Dongyin Wang5Xue Wang6Zhibin Zhang7Jiawei Wen8Xiao Huang9Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaJilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, ChinaInstitute of Science and Technology Information of Jilin Province, Changchun 130033, ChinaInstitute of Science and Technology Information of Jilin Province, Changchun 130033, ChinaSchool of Life Science, Northeast Normal University, Changchun 130024, ChinaJilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, ChinaJilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of China), Changchun 130033, ChinaFruiting body formation in edible fungi is a critical development process for both scientific understanding and industrial cultivation, yet the underlying genetic mechanisms remain poorly elucidated. This study aimed to identify key genes regulating monokaryotic fruiting in <i>Flammulina filiformis</i>. A genetic segregation population was constructed through selfing purification and hybrid segregation of the FF002 strain, followed by mapping candidate genes with bulked segregant analysis sequencing (BSA-seq). A 10 kb genomic region on scaffold19 was identified, pinpointing the gene <i>FV-L110034160</i>, which encodes a U2 snRNP complex component involved in pre-mRNA splicing. A T→G SNP located 121 bp downstream of the ATG codon caused a serine-to-alanine substitution, disrupting a conserved domain and altering fruiting phenotypes. Phylogenetic analysis further revealed conservation of this gene in fungal genera. These findings elucidate a key regulatory gene controlling monokaryotic fruiting in <i>F. filiformis</i>, providing novel insights into fruiting body formation mechanisms and establishing a foundation for genetic studies in other edible fungi.https://www.mdpi.com/2309-608X/11/7/512<i>Flammulina filiformis</i>genetic population constructionBSA-seqmonokaryotic fruiting |
| spellingShingle | Peng Wang Ya Yu Lei Xia Qi Yan Xiao Tan Dongyin Wang Xue Wang Zhibin Zhang Jiawei Wen Xiao Huang Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing Journal of Fungi <i>Flammulina filiformis</i> genetic population construction BSA-seq monokaryotic fruiting |
| title | Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing |
| title_full | Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing |
| title_fullStr | Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing |
| title_full_unstemmed | Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing |
| title_short | Identification of Critical Candidate Genes Controlling Monokaryon Fruiting in <i>Flammulina filiformis</i> Using Genetic Population Construction and Bulked Segregant Analysis Sequencing |
| title_sort | identification of critical candidate genes controlling monokaryon fruiting in i flammulina filiformis i using genetic population construction and bulked segregant analysis sequencing |
| topic | <i>Flammulina filiformis</i> genetic population construction BSA-seq monokaryotic fruiting |
| url | https://www.mdpi.com/2309-608X/11/7/512 |
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