The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers
Air particulate matter (PM) is widely recognized for its potential to negatively affect human health, including changes in the upper respiratory microbiome. However, research on PM-associated microbiota remains limited and mostly focused on PM (e.g., PM2.5 and PM10). This study aims to characterize...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325002106 |
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| author | Giulia Solazzo Sabrina Rovelli Simona Iodice Matthew Chung Michael Frimpong Valentina Bollati Luca Ferrari Elodie Ghedin |
| author_facet | Giulia Solazzo Sabrina Rovelli Simona Iodice Matthew Chung Michael Frimpong Valentina Bollati Luca Ferrari Elodie Ghedin |
| author_sort | Giulia Solazzo |
| collection | DOAJ |
| description | Air particulate matter (PM) is widely recognized for its potential to negatively affect human health, including changes in the upper respiratory microbiome. However, research on PM-associated microbiota remains limited and mostly focused on PM (e.g., PM2.5 and PM10). This study aims to characterize for the first time the microbiome of Total Suspended Particles (TSP) and investigate the correlations of indoor TSP with the human upper respiratory microbiome. Biological and environmental samples were collected over three collection periods lasting three weeks each, between May and July 2022 at the University of Milan and the University of Insubria Como. TSP were sampled using a filter-based technique, while respiratory samples from both anterior nares (AN) and the nasopharynx (NP) were collected using swabs. Microbiome analysis of both human (N = 145) and TSP (N = 51) samples was conducted on metagenomic sequencing data. A comparison of indoor and outdoor TSP microbiomes revealed differences in microbial diversity and taxonomic composition. The indoor samples had higher relative abundance of environmental bacteria often associated with opportunistic infections like Paracoccus sp., as well as respiratory bacteria such as Staphylococcus aureus and Klebsiella pneumoniae. Additionally, both indoor and outdoor TSP samples contained broad spectrum antibiotic resistance genes. Indoor TSP exposure was negatively associated with commensal bacteria and positively associated with Staphylococcus aureus relative abundance. Finally, a correlation between the relative abundance of respiratory bacteria identified in the indoor TSP and the upper respiratory microbiome was found, suggesting a potential interaction between TSP and the upper airways. |
| format | Article |
| id | doaj-art-56a5e3c133174ed590c12041d7c1ca2d |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-56a5e3c133174ed590c12041d7c1ca2d2025-02-10T04:33:30ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117874The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workersGiulia Solazzo0Sabrina Rovelli1Simona Iodice2Matthew Chung3Michael Frimpong4Valentina Bollati5Luca Ferrari6Elodie Ghedin7EPIGET Lab, Department of Clinical Science and Community Health, DIpartimento di Eccellenza 2023-2027, Università degli Studi di Milano; Systems Genomics Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USADepartment of Science and High Technology, Università degli Studi dell'Insubria, Como, ItalyEPIGET Lab, Department of Clinical Science and Community Health, DIpartimento di Eccellenza 2023-2027, Università degli Studi di Milano; Occupational Health Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, ItalySystems Genomics Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USASystems Genomics Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USAEPIGET Lab, Department of Clinical Science and Community Health, DIpartimento di Eccellenza 2023-2027, Università degli Studi di Milano; Occupational Health Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, ItalyEPIGET Lab, Department of Clinical Science and Community Health, DIpartimento di Eccellenza 2023-2027, Università degli Studi di Milano; Occupational Health Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy; Corresponding author at: EPIGET Lab, Department of Clinical Science and Community Health, DIpartimento di Eccellenza 2023-2027, Università degli Studi di Milano.Systems Genomics Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA; Corresponding author.Air particulate matter (PM) is widely recognized for its potential to negatively affect human health, including changes in the upper respiratory microbiome. However, research on PM-associated microbiota remains limited and mostly focused on PM (e.g., PM2.5 and PM10). This study aims to characterize for the first time the microbiome of Total Suspended Particles (TSP) and investigate the correlations of indoor TSP with the human upper respiratory microbiome. Biological and environmental samples were collected over three collection periods lasting three weeks each, between May and July 2022 at the University of Milan and the University of Insubria Como. TSP were sampled using a filter-based technique, while respiratory samples from both anterior nares (AN) and the nasopharynx (NP) were collected using swabs. Microbiome analysis of both human (N = 145) and TSP (N = 51) samples was conducted on metagenomic sequencing data. A comparison of indoor and outdoor TSP microbiomes revealed differences in microbial diversity and taxonomic composition. The indoor samples had higher relative abundance of environmental bacteria often associated with opportunistic infections like Paracoccus sp., as well as respiratory bacteria such as Staphylococcus aureus and Klebsiella pneumoniae. Additionally, both indoor and outdoor TSP samples contained broad spectrum antibiotic resistance genes. Indoor TSP exposure was negatively associated with commensal bacteria and positively associated with Staphylococcus aureus relative abundance. Finally, a correlation between the relative abundance of respiratory bacteria identified in the indoor TSP and the upper respiratory microbiome was found, suggesting a potential interaction between TSP and the upper airways.http://www.sciencedirect.com/science/article/pii/S0147651325002106MicrobiomeIndoor air qualityRespiratory microbiomeEnvironmental bacteria |
| spellingShingle | Giulia Solazzo Sabrina Rovelli Simona Iodice Matthew Chung Michael Frimpong Valentina Bollati Luca Ferrari Elodie Ghedin The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers Ecotoxicology and Environmental Safety Microbiome Indoor air quality Respiratory microbiome Environmental bacteria |
| title | The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers |
| title_full | The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers |
| title_fullStr | The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers |
| title_full_unstemmed | The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers |
| title_short | The microbiome of Total Suspended Particles and its influence on the respiratory microbiome of healthy office workers |
| title_sort | microbiome of total suspended particles and its influence on the respiratory microbiome of healthy office workers |
| topic | Microbiome Indoor air quality Respiratory microbiome Environmental bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325002106 |
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