The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology
The bulbs of the lily plant <i>Fritillaria thunbergii</i> Miq. possess substantial medicinal properties for relieving coughs and clearing the lungs. However, excessive pursuit of yield during cultivation has led to a decrease in medicinal ingredients. Therefore, we aimed to investigate t...
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2025-01-01
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| author | Leran Wang Bingbing Liang Jia Liu Huizhen Jin Zixuan Zhu Siyu Hao Shumin Wang Xiaoxiao Sheng Xinshu Zhou Honghai Zhu Ning Sui |
| author_facet | Leran Wang Bingbing Liang Jia Liu Huizhen Jin Zixuan Zhu Siyu Hao Shumin Wang Xiaoxiao Sheng Xinshu Zhou Honghai Zhu Ning Sui |
| author_sort | Leran Wang |
| collection | DOAJ |
| description | The bulbs of the lily plant <i>Fritillaria thunbergii</i> Miq. possess substantial medicinal properties for relieving coughs and clearing the lungs. However, excessive pursuit of yield during cultivation has led to a decrease in medicinal ingredients. Therefore, we aimed to investigate the effects of two single-factor treatments, shading (SK0) and potassium application (S0K), and their coupling treatment (SK) on bulb biomass and medicinal substance content, along with the role of rhizosphere microorganisms. Shading increased the content of active ingredients in bulbs by approximately 11.7% while decreasing bulb biomass by approximately 11.3%. SK treatment mitigated the biomass reduction caused by SK0 treatment while enhancing the accumulation of active ingredients in <i>F. thunbergii</i>, up to 1.2 times higher than that of SK0 treatment. In rhizosphere soil, <i>Allorhizobium–Neorhizobium–Pararhizobium–Rhizobium</i> (ANPR), <i>Chryseobacterium</i>, <i>Brevundimonas</i>, and <i>Phoma</i> exhibited significant positive correlations with medicinal components, among which ANPR, <i>Brevundimonas, Chryseobacterium,</i> and <i>Phoma</i> were responsive to SK treatments. Also, <i>Burkholderia–Caballeronia–Paraburkholderia</i> (BCP) and <i>Brevundimonas</i> responded to changes at different growth stages of <i>F. thunbergii.</i> The relative abundance of these microorganisms was associated with the alterations of soil factors resulting from shading or K application. Our results indicate that these microorganisms are beneficial to the growth of bulbs and the synthesis of active components in <i>F. thunbergii.</i> The combination of shading and K application may regulate the accumulation of medicinal substances in <i>F. thunbergii</i> by modulating the structure of the soil microbial community. Our results serve as a reference for soil improvement for medicinal plant cultivation. |
| format | Article |
| id | doaj-art-569fba46520d4074be92e036a228c176 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Microorganisms |
| spelling | doaj-art-569fba46520d4074be92e036a228c1762025-01-24T13:42:44ZengMDPI AGMicroorganisms2076-26072025-01-0113112510.3390/microorganisms13010125The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere MicroecologyLeran Wang0Bingbing Liang1Jia Liu2Huizhen Jin3Zixuan Zhu4Siyu Hao5Shumin Wang6Xiaoxiao Sheng7Xinshu Zhou8Honghai Zhu9Ning Sui10School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaThe bulbs of the lily plant <i>Fritillaria thunbergii</i> Miq. possess substantial medicinal properties for relieving coughs and clearing the lungs. However, excessive pursuit of yield during cultivation has led to a decrease in medicinal ingredients. Therefore, we aimed to investigate the effects of two single-factor treatments, shading (SK0) and potassium application (S0K), and their coupling treatment (SK) on bulb biomass and medicinal substance content, along with the role of rhizosphere microorganisms. Shading increased the content of active ingredients in bulbs by approximately 11.7% while decreasing bulb biomass by approximately 11.3%. SK treatment mitigated the biomass reduction caused by SK0 treatment while enhancing the accumulation of active ingredients in <i>F. thunbergii</i>, up to 1.2 times higher than that of SK0 treatment. In rhizosphere soil, <i>Allorhizobium–Neorhizobium–Pararhizobium–Rhizobium</i> (ANPR), <i>Chryseobacterium</i>, <i>Brevundimonas</i>, and <i>Phoma</i> exhibited significant positive correlations with medicinal components, among which ANPR, <i>Brevundimonas, Chryseobacterium,</i> and <i>Phoma</i> were responsive to SK treatments. Also, <i>Burkholderia–Caballeronia–Paraburkholderia</i> (BCP) and <i>Brevundimonas</i> responded to changes at different growth stages of <i>F. thunbergii.</i> The relative abundance of these microorganisms was associated with the alterations of soil factors resulting from shading or K application. Our results indicate that these microorganisms are beneficial to the growth of bulbs and the synthesis of active components in <i>F. thunbergii.</i> The combination of shading and K application may regulate the accumulation of medicinal substances in <i>F. thunbergii</i> by modulating the structure of the soil microbial community. Our results serve as a reference for soil improvement for medicinal plant cultivation.https://www.mdpi.com/2076-2607/13/1/125<i>Fritillaria thunbergii</i> Miq.rhizosphere microorganismsactive ingredientsshadepotassium application |
| spellingShingle | Leran Wang Bingbing Liang Jia Liu Huizhen Jin Zixuan Zhu Siyu Hao Shumin Wang Xiaoxiao Sheng Xinshu Zhou Honghai Zhu Ning Sui The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology Microorganisms <i>Fritillaria thunbergii</i> Miq. rhizosphere microorganisms active ingredients shade potassium application |
| title | The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology |
| title_full | The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology |
| title_fullStr | The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology |
| title_full_unstemmed | The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology |
| title_short | The Combination of Shading and Potassium Application Regulated the Bulb Active Ingredients Accumulation in <i>Fritillaria thunbergii</i> Miq. by Affecting Rhizosphere Microecology |
| title_sort | combination of shading and potassium application regulated the bulb active ingredients accumulation in i fritillaria thunbergii i miq by affecting rhizosphere microecology |
| topic | <i>Fritillaria thunbergii</i> Miq. rhizosphere microorganisms active ingredients shade potassium application |
| url | https://www.mdpi.com/2076-2607/13/1/125 |
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