Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou
A filamentous virus was isolated from naturally infected squash (Cucuribita moschata L.), showing yellow mosaic in Hangzhou, Zhejiang Province, in autumn 1999. This virus was found to cause systemic symptoms on all the Cucuribitaceae plants tested. It was identified as zucchini yellow mosaic virus (...
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| Language: | English |
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Zhejiang University Press
2003-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0539 |
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| author | CHEN Jie-yun CHAI Li-hong CHEN Ji-shuang HONG Jian |
| author_facet | CHEN Jie-yun CHAI Li-hong CHEN Ji-shuang HONG Jian |
| author_sort | CHEN Jie-yun |
| collection | DOAJ |
| description | A filamentous virus was isolated from naturally infected squash (Cucuribita moschata L.), showing yellow mosaic in Hangzhou, Zhejiang Province, in autumn 1999. This virus was found to cause systemic symptoms on all the Cucuribitaceae plants tested. It was identified as zucchini yellow mosaic virus (ZYMV) by host reaction tests, morphological characteristics observations and tissue alteration tests. The 3' end sequence, containing the coat protein (CP) gene and the 3' untranslated region of this virus, was obtained by RT-PCR and sequenced. Sequence alignment of CP genes was carried out in compare with three American ZYMV isolates, including the Conneticut isolate, Florida isolate and California isolate, and Tonga isolate of Watermelon mosaic virus 2 (WMV2). ZYMV HZ00s1 had 93%-94% homology of nucleotide sequence with other three documented ZYMV isolates, whereas its sequence similarity to WMV2 Tonga isolate was found to be as low as 67%. Meanwhile, sequence similarity between the three America ZYMV isolates was 95% to 99%. Homology of deduced amino acid sequence of CP between HZ00s1 and three other ZYMV isolates was 95%-98%. Our results proved the occurrence of ZYMV as a principal pathogenic virus on Cucuribitaceae crops in South part of China. |
| format | Article |
| id | doaj-art-5690a47a730a401ebb2345c68aec1870 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2003-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-5690a47a730a401ebb2345c68aec18702025-08-20T03:15:38ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-09-012953954410.3785/1008-9209.2003.05.053910089209Identification of a zucchini yellow mosaic virus infecting squash in HangzhouCHEN Jie-yunCHAI Li-hongCHEN Ji-shuangHONG JianA filamentous virus was isolated from naturally infected squash (Cucuribita moschata L.), showing yellow mosaic in Hangzhou, Zhejiang Province, in autumn 1999. This virus was found to cause systemic symptoms on all the Cucuribitaceae plants tested. It was identified as zucchini yellow mosaic virus (ZYMV) by host reaction tests, morphological characteristics observations and tissue alteration tests. The 3' end sequence, containing the coat protein (CP) gene and the 3' untranslated region of this virus, was obtained by RT-PCR and sequenced. Sequence alignment of CP genes was carried out in compare with three American ZYMV isolates, including the Conneticut isolate, Florida isolate and California isolate, and Tonga isolate of Watermelon mosaic virus 2 (WMV2). ZYMV HZ00s1 had 93%-94% homology of nucleotide sequence with other three documented ZYMV isolates, whereas its sequence similarity to WMV2 Tonga isolate was found to be as low as 67%. Meanwhile, sequence similarity between the three America ZYMV isolates was 95% to 99%. Homology of deduced amino acid sequence of CP between HZ00s1 and three other ZYMV isolates was 95%-98%. Our results proved the occurrence of ZYMV as a principal pathogenic virus on Cucuribitaceae crops in South part of China.https://www.academax.com/doi/10.3785/1008-9209.2003.05.0539ZYMVvirus identification3' end sequencecoat proteinsimilarity comparison |
| spellingShingle | CHEN Jie-yun CHAI Li-hong CHEN Ji-shuang HONG Jian Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou 浙江大学学报. 农业与生命科学版 ZYMV virus identification 3' end sequence coat protein similarity comparison |
| title | Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou |
| title_full | Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou |
| title_fullStr | Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou |
| title_full_unstemmed | Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou |
| title_short | Identification of a zucchini yellow mosaic virus infecting squash in Hangzhou |
| title_sort | identification of a zucchini yellow mosaic virus infecting squash in hangzhou |
| topic | ZYMV virus identification 3' end sequence coat protein similarity comparison |
| url | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0539 |
| work_keys_str_mv | AT chenjieyun identificationofazucchiniyellowmosaicvirusinfectingsquashinhangzhou AT chailihong identificationofazucchiniyellowmosaicvirusinfectingsquashinhangzhou AT chenjishuang identificationofazucchiniyellowmosaicvirusinfectingsquashinhangzhou AT hongjian identificationofazucchiniyellowmosaicvirusinfectingsquashinhangzhou |