Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis
Pulpitis is an important and prevalent disease within the oral cavity. Thus, animal models are necessary tools for basic research focused on pulpitis. Researchers worldwide often use dogs and miniature pigs to construct animal models of pulpitis. However, gene editing in miniature pigs is difficult,...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2025-01-01
|
| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5128&type=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206622962581504 |
|---|---|
| author | Lanting Shao Baian Chen Ying Zheng |
| author_facet | Lanting Shao Baian Chen Ying Zheng |
| author_sort | Lanting Shao |
| collection | DOAJ |
| description | Pulpitis is an important and prevalent disease within the oral cavity. Thus, animal models are necessary tools for basic research focused on pulpitis. Researchers worldwide often use dogs and miniature pigs to construct animal models of pulpitis. However, gene editing in miniature pigs is difficult, the surgical modeling process is complex, and tooth demineralization time is lengthy. Although some researchers have attempted to establish a mouse model of pulpitis, most models have involved direct exposure of dental pulp. However, the causes of pulpitis vary considerably among individuals, hindering effective research. In this study, we established a mouse model of pulpitis by accessing the pulp cavity, exposing the pulp to lipopolysaccharide (LPS), and then filling the tooth. One day after surgery, we observed many necrotic tissues and extensive inflammatory exudate, including neutrophils, around the coronal cavity preparation. Additionally, we noted many more neutrophils and a small amount of chronic inflammatory cell infiltrates at the junction between inflamed and normal tissue. These findings indicated that our model can be used to explore the early stage of pulpitis. Ten days after surgery, we observed vacuolar degeneration in some fibroblasts and proliferation in others at the distal end of the inflamed tissue. We also noted dilation and congestion of the pulp blood vessels. Therefore, our model can also be used to explore the middle and later stages of pulpitis. Thirty days after surgery, we observed necrosis in the coronal pulp cavity and upper half of the root pulp, indicating that our model can also be used to explore the end stage of pulpitis. This model is easy to establish, shows pulpitis progression in the dental pulp, exhibits a clear inflammatory phenotype, and can be readily combined with gene editing techniques. Accordingly, it is suitable for basic research focused on pulpitis and has substantial practical value. |
| format | Article |
| id | doaj-art-568c7aac4e3344958e3db8ebc0211298 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-568c7aac4e3344958e3db8ebc02112982025-02-07T08:16:38ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115210.21769/BioProtoc.5128Mouse Model of Lipopolysaccharide (LPS)-Induced PulpitisLanting Shao0Baian Chen1Ying Zheng2School of Basic Medical Sciences, Capital Medical University, Beijing, ChinaSchool of Basic Medical Sciences, Capital Medical University, Beijing, ChinaDepartment of Stomatology, Peking Union Medical College Hospital, Beijing, ChinaPulpitis is an important and prevalent disease within the oral cavity. Thus, animal models are necessary tools for basic research focused on pulpitis. Researchers worldwide often use dogs and miniature pigs to construct animal models of pulpitis. However, gene editing in miniature pigs is difficult, the surgical modeling process is complex, and tooth demineralization time is lengthy. Although some researchers have attempted to establish a mouse model of pulpitis, most models have involved direct exposure of dental pulp. However, the causes of pulpitis vary considerably among individuals, hindering effective research. In this study, we established a mouse model of pulpitis by accessing the pulp cavity, exposing the pulp to lipopolysaccharide (LPS), and then filling the tooth. One day after surgery, we observed many necrotic tissues and extensive inflammatory exudate, including neutrophils, around the coronal cavity preparation. Additionally, we noted many more neutrophils and a small amount of chronic inflammatory cell infiltrates at the junction between inflamed and normal tissue. These findings indicated that our model can be used to explore the early stage of pulpitis. Ten days after surgery, we observed vacuolar degeneration in some fibroblasts and proliferation in others at the distal end of the inflamed tissue. We also noted dilation and congestion of the pulp blood vessels. Therefore, our model can also be used to explore the middle and later stages of pulpitis. Thirty days after surgery, we observed necrosis in the coronal pulp cavity and upper half of the root pulp, indicating that our model can also be used to explore the end stage of pulpitis. This model is easy to establish, shows pulpitis progression in the dental pulp, exhibits a clear inflammatory phenotype, and can be readily combined with gene editing techniques. Accordingly, it is suitable for basic research focused on pulpitis and has substantial practical value.https://bio-protocol.org/en/bpdetail?id=5128&type=0 |
| spellingShingle | Lanting Shao Baian Chen Ying Zheng Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis Bio-Protocol |
| title | Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis |
| title_full | Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis |
| title_fullStr | Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis |
| title_full_unstemmed | Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis |
| title_short | Mouse Model of Lipopolysaccharide (LPS)-Induced Pulpitis |
| title_sort | mouse model of lipopolysaccharide lps induced pulpitis |
| url | https://bio-protocol.org/en/bpdetail?id=5128&type=0 |
| work_keys_str_mv | AT lantingshao mousemodeloflipopolysaccharidelpsinducedpulpitis AT baianchen mousemodeloflipopolysaccharidelpsinducedpulpitis AT yingzheng mousemodeloflipopolysaccharidelpsinducedpulpitis |