Isolated cell behavior drives the evolution of antibiotic resistance
Abstract Bacterial antibiotic resistance is typically quantified by the minimum inhibitory concentration (MIC), which is defined as the minimal concentration of antibiotic that inhibits bacterial growth starting from a standard cell density. However, when antibiotic resistance is mediated by degrada...
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| Format: | Article |
| Language: | English |
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Springer Nature
2015-07-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.15252/msb.20145888 |
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| _version_ | 1849331540017807360 |
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| author | Tatiana Artemova Ylaine Gerardin Carmel Dudley Nicole M Vega Jeff Gore |
| author_facet | Tatiana Artemova Ylaine Gerardin Carmel Dudley Nicole M Vega Jeff Gore |
| author_sort | Tatiana Artemova |
| collection | DOAJ |
| description | Abstract Bacterial antibiotic resistance is typically quantified by the minimum inhibitory concentration (MIC), which is defined as the minimal concentration of antibiotic that inhibits bacterial growth starting from a standard cell density. However, when antibiotic resistance is mediated by degradation, the collective inactivation of antibiotic by the bacterial population can cause the measured MIC to depend strongly on the initial cell density. In cases where this inoculum effect is strong, the relationship between MIC and bacterial fitness in the antibiotic is not well defined. Here, we demonstrate that the resistance of a single, isolated cell—which we call the single‐cell MIC (scMIC)—provides a superior metric for quantifying antibiotic resistance. Unlike the MIC, we find that the scMIC predicts the direction of selection and also specifies the antibiotic concentration at which selection begins to favor new mutants. Understanding the cooperative nature of bacterial growth in antibiotics is therefore essential in predicting the evolution of antibiotic resistance. |
| format | Article |
| id | doaj-art-568a3ca8574e494db8250f3afd2964f7 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-568a3ca8574e494db8250f3afd2964f72025-08-20T03:46:32ZengSpringer NatureMolecular Systems Biology1744-42922015-07-0111711110.15252/msb.20145888Isolated cell behavior drives the evolution of antibiotic resistanceTatiana Artemova0Ylaine Gerardin1Carmel Dudley2Nicole M Vega3Jeff Gore4Department of Physics, Massachusetts Institute of TechnologyDepartment of Systems Biology, Harvard Medical SchoolDepartment of Physics, Massachusetts Institute of TechnologyDepartment of Physics, Massachusetts Institute of TechnologyDepartment of Physics, Massachusetts Institute of TechnologyAbstract Bacterial antibiotic resistance is typically quantified by the minimum inhibitory concentration (MIC), which is defined as the minimal concentration of antibiotic that inhibits bacterial growth starting from a standard cell density. However, when antibiotic resistance is mediated by degradation, the collective inactivation of antibiotic by the bacterial population can cause the measured MIC to depend strongly on the initial cell density. In cases where this inoculum effect is strong, the relationship between MIC and bacterial fitness in the antibiotic is not well defined. Here, we demonstrate that the resistance of a single, isolated cell—which we call the single‐cell MIC (scMIC)—provides a superior metric for quantifying antibiotic resistance. Unlike the MIC, we find that the scMIC predicts the direction of selection and also specifies the antibiotic concentration at which selection begins to favor new mutants. Understanding the cooperative nature of bacterial growth in antibiotics is therefore essential in predicting the evolution of antibiotic resistance.https://doi.org/10.15252/msb.20145888antibiotic resistancebeta‐lactamasecooperative growthevolution |
| spellingShingle | Tatiana Artemova Ylaine Gerardin Carmel Dudley Nicole M Vega Jeff Gore Isolated cell behavior drives the evolution of antibiotic resistance Molecular Systems Biology antibiotic resistance beta‐lactamase cooperative growth evolution |
| title | Isolated cell behavior drives the evolution of antibiotic resistance |
| title_full | Isolated cell behavior drives the evolution of antibiotic resistance |
| title_fullStr | Isolated cell behavior drives the evolution of antibiotic resistance |
| title_full_unstemmed | Isolated cell behavior drives the evolution of antibiotic resistance |
| title_short | Isolated cell behavior drives the evolution of antibiotic resistance |
| title_sort | isolated cell behavior drives the evolution of antibiotic resistance |
| topic | antibiotic resistance beta‐lactamase cooperative growth evolution |
| url | https://doi.org/10.15252/msb.20145888 |
| work_keys_str_mv | AT tatianaartemova isolatedcellbehaviordrivestheevolutionofantibioticresistance AT ylainegerardin isolatedcellbehaviordrivestheevolutionofantibioticresistance AT carmeldudley isolatedcellbehaviordrivestheevolutionofantibioticresistance AT nicolemvega isolatedcellbehaviordrivestheevolutionofantibioticresistance AT jeffgore isolatedcellbehaviordrivestheevolutionofantibioticresistance |