Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color

ABSTRACT Cities impose unique selection pressures on wildlife and generate clines in phenotypic traits along urban–rural gradients. Roads are a widespread feature of human‐dominated landscapes and are known to cause direct wildlife mortality; however, whether they act as a selective force influencin...

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Main Authors: Adam F. Parlin, Bradley J. Cosentino, Richard M. Lehtinen, John E. McDonald, Emma C. C. Sinclair, James P. Gibbs
Format: Article
Language:English
Published: Wiley 2025-05-01
Series:Evolutionary Applications
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Online Access:https://doi.org/10.1111/eva.70109
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author Adam F. Parlin
Bradley J. Cosentino
Richard M. Lehtinen
John E. McDonald
Emma C. C. Sinclair
James P. Gibbs
author_facet Adam F. Parlin
Bradley J. Cosentino
Richard M. Lehtinen
John E. McDonald
Emma C. C. Sinclair
James P. Gibbs
author_sort Adam F. Parlin
collection DOAJ
description ABSTRACT Cities impose unique selection pressures on wildlife and generate clines in phenotypic traits along urban–rural gradients. Roads are a widespread feature of human‐dominated landscapes and are known to cause direct wildlife mortality; however, whether they act as a selective force influencing phenotypic trait variation along urban–rural gradients remains unclear. This study tested the hypothesis that roads influence natural selection of coat color in the eastern gray squirrel (Sciurus carolinensis), a species with two distinct coat colors: a gray morph that is common in all areas and a melanic morph more prevalent in urban areas than in rural ones. Vehicular collisions are a significant cause of mortality in eastern gray squirrels, with the melanic morph more visually conspicuous on roads and more easily detected and avoided by drivers than the gray morph. Standardized road cruise surveys along an urbanization gradient in Syracuse, New York, USA, revealed that the prevalence of melanism among living squirrels in Syracuse was negatively related to distance from the city center, whereas there was no urban–rural cline in melanism among road‐killed individuals, with the melanic morph underrepresented among road‐killed squirrels by up to 30% along the urbanization gradient. An examination of the prevalence of each color morph on and off road surfaces in a range‐wide compilation of > 100,000 photographs of S. carolinensis also indicated that the melanic morph was underrepresented among road‐killed squirrels imaged. Our study highlights vehicular collisions as an important source of natural selection on phenotypic traits, suggesting a potential role in shaping patterns of urban evolution and contributing to the maintenance of urban–rural clines.
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spelling doaj-art-56840d472e2c4b298552bf5b2c7c59cc2025-08-20T03:12:26ZengWileyEvolutionary Applications1752-45712025-05-01185n/an/a10.1111/eva.70109Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat ColorAdam F. Parlin0Bradley J. Cosentino1Richard M. Lehtinen2John E. McDonald3Emma C. C. Sinclair4James P. Gibbs5Department of Environmental Biology State University of New York College of Environmental Science and Forestry Syracuse New York USADepartment of Biology Hobart & William Smith Colleges Geneva New York USADepartment of Biology The College of Wooster Wooster Ohio USADepartment of Environmental Science Westfield State University Westfield Massachusetts USADepartment of Biology Queen's University Kingston Ontario CanadaDepartment of Environmental Biology State University of New York College of Environmental Science and Forestry Syracuse New York USAABSTRACT Cities impose unique selection pressures on wildlife and generate clines in phenotypic traits along urban–rural gradients. Roads are a widespread feature of human‐dominated landscapes and are known to cause direct wildlife mortality; however, whether they act as a selective force influencing phenotypic trait variation along urban–rural gradients remains unclear. This study tested the hypothesis that roads influence natural selection of coat color in the eastern gray squirrel (Sciurus carolinensis), a species with two distinct coat colors: a gray morph that is common in all areas and a melanic morph more prevalent in urban areas than in rural ones. Vehicular collisions are a significant cause of mortality in eastern gray squirrels, with the melanic morph more visually conspicuous on roads and more easily detected and avoided by drivers than the gray morph. Standardized road cruise surveys along an urbanization gradient in Syracuse, New York, USA, revealed that the prevalence of melanism among living squirrels in Syracuse was negatively related to distance from the city center, whereas there was no urban–rural cline in melanism among road‐killed individuals, with the melanic morph underrepresented among road‐killed squirrels by up to 30% along the urbanization gradient. An examination of the prevalence of each color morph on and off road surfaces in a range‐wide compilation of > 100,000 photographs of S. carolinensis also indicated that the melanic morph was underrepresented among road‐killed squirrels imaged. Our study highlights vehicular collisions as an important source of natural selection on phenotypic traits, suggesting a potential role in shaping patterns of urban evolution and contributing to the maintenance of urban–rural clines.https://doi.org/10.1111/eva.70109adaptive evolutionanimal colorcitizen sciencenatural selectionphenotypic variationroad ecology
spellingShingle Adam F. Parlin
Bradley J. Cosentino
Richard M. Lehtinen
John E. McDonald
Emma C. C. Sinclair
James P. Gibbs
Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color
Evolutionary Applications
adaptive evolution
animal color
citizen science
natural selection
phenotypic variation
road ecology
title Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color
title_full Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color
title_fullStr Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color
title_full_unstemmed Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color
title_short Road Mortality Contributes to the Evolution of an Urban–Rural Cline in Squirrel Coat Color
title_sort road mortality contributes to the evolution of an urban rural cline in squirrel coat color
topic adaptive evolution
animal color
citizen science
natural selection
phenotypic variation
road ecology
url https://doi.org/10.1111/eva.70109
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