Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia
Agrobacterium vitis causes common grape vine (Vitis vinifera L.) crown gall disease that destroyed a lot of Slovenian vineyards more than a decade ago. Eighty isolates of Agrobacterium spp. collected during monitoring in 2006 were identified as A. vitis and A. tumefacies by pehA and multiplex PCR me...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2014-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/12626 |
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| author | Janja LAMOVŠEK Igor ZIDARIČ Irena MAVRIČ PLEŠKO Gregor UREK Stanislav TRDAN |
| author_facet | Janja LAMOVŠEK Igor ZIDARIČ Irena MAVRIČ PLEŠKO Gregor UREK Stanislav TRDAN |
| author_sort | Janja LAMOVŠEK |
| collection | DOAJ |
| description | Agrobacterium vitis causes common grape vine (Vitis vinifera L.) crown gall disease that destroyed a lot of Slovenian vineyards more than a decade ago. Eighty isolates of Agrobacterium spp. collected during monitoring in 2006 were identified as A. vitis and A. tumefacies by pehA and multiplex PCR method. Tumor-inducing capacity of these strains was assessed on test plants and with PCR methods for detection of the Ti plasmid responsible for tumor induction. With VCF3/VCR3 primer pair six false negatives and no false positives were detected. The high genetic diversity of pathogenic Agrobacterium spp. strains affects the performance of molecular methods, thus biological test should be performed where results from molecular methods are doubtful. |
| format | Article |
| id | doaj-art-5678eec93d6d479f965cd7004f2fa257 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-5678eec93d6d479f965cd7004f2fa2572025-08-20T03:02:25ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412014-12-01103231332110.14720//aas.2014.103.2.1619018Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in SloveniaJanja LAMOVŠEK0Igor ZIDARIČ1Irena MAVRIČ PLEŠKO2Gregor UREK3Stanislav TRDAN4Agricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaAgricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaAgricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaAgricultural Institute of Slovenia, Plant Protection Department, Hacquetova ulica 17, SI-1000 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical Faculty, Dept. of Agronomy, Jamnikarjeva 101, SI-1111 Ljubljana, SloveniaAgrobacterium vitis causes common grape vine (Vitis vinifera L.) crown gall disease that destroyed a lot of Slovenian vineyards more than a decade ago. Eighty isolates of Agrobacterium spp. collected during monitoring in 2006 were identified as A. vitis and A. tumefacies by pehA and multiplex PCR method. Tumor-inducing capacity of these strains was assessed on test plants and with PCR methods for detection of the Ti plasmid responsible for tumor induction. With VCF3/VCR3 primer pair six false negatives and no false positives were detected. The high genetic diversity of pathogenic Agrobacterium spp. strains affects the performance of molecular methods, thus biological test should be performed where results from molecular methods are doubtful.https://journals.uni-lj.si/aas/article/view/12626vitis viniferagrapevinesagrobacteriumhostsvineyardsdamagegeneticsgenetic variationpathogenicitymolecular biologyplasmidscankers |
| spellingShingle | Janja LAMOVŠEK Igor ZIDARIČ Irena MAVRIČ PLEŠKO Gregor UREK Stanislav TRDAN Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia Acta Agriculturae Slovenica vitis vinifera grapevines agrobacterium hosts vineyards damage genetics genetic variation pathogenicity molecular biology plasmids cankers |
| title | Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia |
| title_full | Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia |
| title_fullStr | Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia |
| title_full_unstemmed | Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia |
| title_short | Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia |
| title_sort | comparative study of diagnostic methods used for monitoring of common grape vine vitis vinifera l crown gall agrobacterium vitis ophel kerr in slovenia |
| topic | vitis vinifera grapevines agrobacterium hosts vineyards damage genetics genetic variation pathogenicity molecular biology plasmids cankers |
| url | https://journals.uni-lj.si/aas/article/view/12626 |
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