Trema micranthum (L.) Blume as a new source of cannabinoids
Abstract Trema micranthum (Cannabaceae) has emerged as a promising new source of cannabinoids, including cannabidiol (CBD). Given the substantial medicinal demand for cannabinoids and the regulatory challenges associated with Cannabis sativa due to the presence of Δ9-tetrahydrocannabinol (THC), this...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-80857-6 |
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| author | Rayssa Ribeiro Yasmin Cunha da Silva Ricardo Finotti Gabriel Reis Alves Carneiro Gustavo Ramalho Cardoso dos Santos Henrique Marcelo Gualberto Pereira Monica Costa Padilha Valdir F. Veiga-Junior |
| author_facet | Rayssa Ribeiro Yasmin Cunha da Silva Ricardo Finotti Gabriel Reis Alves Carneiro Gustavo Ramalho Cardoso dos Santos Henrique Marcelo Gualberto Pereira Monica Costa Padilha Valdir F. Veiga-Junior |
| author_sort | Rayssa Ribeiro |
| collection | DOAJ |
| description | Abstract Trema micranthum (Cannabaceae) has emerged as a promising new source of cannabinoids, including cannabidiol (CBD). Given the substantial medicinal demand for cannabinoids and the regulatory challenges associated with Cannabis sativa due to the presence of Δ9-tetrahydrocannabinol (THC), this study sought to explore the presence of CBD, THC, and their precursors, Δ9-tetrahydrocannabinolic acid A (THCA A) and cannabidiolic acid (CBDA), in various parts of Trema micranthum using UHPLC-HRMS/MS (Orbitrap). Extracts from fruits, leaves, inflorescences, and stems were obtained using a methanol/hexane (9:1, v/v) solvent mixture. UHPLC coupled with an Orbitrap mass spectrometer was employed for cannabinoid identification and quantification, with standard mixtures prepared in methanol. The extracts yielded significant quantities, such as 6.6%/g from leaves and 3%/g from fruits. Cannabinoids were detected in fruits, leaves, and inflorescences, with acidic forms (CBDA and THCA A) present in higher concentrations than their neutral counterparts. Notably, leaves contained 4.43 × 10−3 µg/g of CBD and 1.05 × 10−3 µg/g of THC. These findings, facilitated by high-resolution analytical methods, underscore the potential of Trema micranthum as an alternative source for cannabinoids, guiding future research in this area. |
| format | Article |
| id | doaj-art-5675ffaf062d45bdb722635f606e79e0 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5675ffaf062d45bdb722635f606e79e02025-08-20T02:49:17ZengNature PortfolioScientific Reports2045-23222024-11-011411910.1038/s41598-024-80857-6Trema micranthum (L.) Blume as a new source of cannabinoidsRayssa Ribeiro0Yasmin Cunha da Silva1Ricardo Finotti2Gabriel Reis Alves Carneiro3Gustavo Ramalho Cardoso dos Santos4Henrique Marcelo Gualberto Pereira5Monica Costa Padilha6Valdir F. Veiga-Junior7Department of Chemical Engineering, Military Institute of EngineeringDepartment of Chemical Engineering, Military Institute of EngineeringDepartment of Biology, Universidade Estácio de Sá (UNESA)Chemistry Institute, Federal University of Rio de Janeiro, Brazilian Doping Control Laboratory (LBCD/IQ-UFRJ)Chemistry Institute, Federal University of Rio de Janeiro, Brazilian Doping Control Laboratory (LBCD/IQ-UFRJ)Chemistry Institute, Federal University of Rio de Janeiro, Brazilian Doping Control Laboratory (LBCD/IQ-UFRJ)Chemistry Institute, Federal University of Rio de Janeiro, Brazilian Doping Control Laboratory (LBCD/IQ-UFRJ)Department of Chemical Engineering, Military Institute of EngineeringAbstract Trema micranthum (Cannabaceae) has emerged as a promising new source of cannabinoids, including cannabidiol (CBD). Given the substantial medicinal demand for cannabinoids and the regulatory challenges associated with Cannabis sativa due to the presence of Δ9-tetrahydrocannabinol (THC), this study sought to explore the presence of CBD, THC, and their precursors, Δ9-tetrahydrocannabinolic acid A (THCA A) and cannabidiolic acid (CBDA), in various parts of Trema micranthum using UHPLC-HRMS/MS (Orbitrap). Extracts from fruits, leaves, inflorescences, and stems were obtained using a methanol/hexane (9:1, v/v) solvent mixture. UHPLC coupled with an Orbitrap mass spectrometer was employed for cannabinoid identification and quantification, with standard mixtures prepared in methanol. The extracts yielded significant quantities, such as 6.6%/g from leaves and 3%/g from fruits. Cannabinoids were detected in fruits, leaves, and inflorescences, with acidic forms (CBDA and THCA A) present in higher concentrations than their neutral counterparts. Notably, leaves contained 4.43 × 10−3 µg/g of CBD and 1.05 × 10−3 µg/g of THC. These findings, facilitated by high-resolution analytical methods, underscore the potential of Trema micranthum as an alternative source for cannabinoids, guiding future research in this area.https://doi.org/10.1038/s41598-024-80857-6CannabaceaeTrema micranthumCannabinoidsCBD/THCUHPLC-HRMS/MS |
| spellingShingle | Rayssa Ribeiro Yasmin Cunha da Silva Ricardo Finotti Gabriel Reis Alves Carneiro Gustavo Ramalho Cardoso dos Santos Henrique Marcelo Gualberto Pereira Monica Costa Padilha Valdir F. Veiga-Junior Trema micranthum (L.) Blume as a new source of cannabinoids Scientific Reports Cannabaceae Trema micranthum Cannabinoids CBD/THC UHPLC-HRMS/MS |
| title | Trema micranthum (L.) Blume as a new source of cannabinoids |
| title_full | Trema micranthum (L.) Blume as a new source of cannabinoids |
| title_fullStr | Trema micranthum (L.) Blume as a new source of cannabinoids |
| title_full_unstemmed | Trema micranthum (L.) Blume as a new source of cannabinoids |
| title_short | Trema micranthum (L.) Blume as a new source of cannabinoids |
| title_sort | trema micranthum l blume as a new source of cannabinoids |
| topic | Cannabaceae Trema micranthum Cannabinoids CBD/THC UHPLC-HRMS/MS |
| url | https://doi.org/10.1038/s41598-024-80857-6 |
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