Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i>
Phenolic compounds, such as stilbenes and flavonoids, from <i>Dalea</i> spp. exhibit diverse biological activities, including antimicrobial, anti-inflammatory, and cytotoxicity properties. To this end, the objectives of this study were to establish hairy root cultures of <i>D. purp...
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2025-01-01
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| author | Amit Raj Sharma Gaurav Gajurel Salma Abdel-Karim Mohammad Abrar Alam Robert Colquhoun Shields Fabricio Medina-Bolivar |
| author_facet | Amit Raj Sharma Gaurav Gajurel Salma Abdel-Karim Mohammad Abrar Alam Robert Colquhoun Shields Fabricio Medina-Bolivar |
| author_sort | Amit Raj Sharma |
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| description | Phenolic compounds, such as stilbenes and flavonoids, from <i>Dalea</i> spp. exhibit diverse biological activities, including antimicrobial, anti-inflammatory, and cytotoxicity properties. To this end, the objectives of this study were to establish hairy root cultures of <i>D. purpurea</i> and assess its capacity to produce these bioactive compounds. The co-treatment of the hairy root cultures with the elicitors methyl-β-cyclodextrin, magnesium chloride, methyl jasmonate, and hydrogen peroxide led to the production and secretion of the geranylated flavanone malheuran A, which was confirmed by mass spectrometry and NMR spectroscopy analyses. The highest amount (104.3 ± 6.9 mg/L) of malheuran A was detected at 192 h after elicitor treatment. The elicited hairy roots were re-elicited for 192 h with the same combination of elicitors, and they produced a substantial amount of malheuran A (91.9 ± 6.8 mg/L). Malheuran A, purified from extracts of the hairy root culture medium, showed potent antimicrobial activity against Gram-positive bacteria, including methicillin-resistant <i>Staphylococcus aureus</i> and vancomycin-resistant Enterococci. It also demonstrated anti-inflammatory effects by suppressing nitrite production in LPS-stimulated RAW 264.7 macrophages. These findings show that various elicitor treatments can boost malheuran A production in hairy root cultures, making them a sustainable bioproduction platform for this bioactive specialized metabolite. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-566ad11328d64e9b87a35cffb53294092025-01-24T13:46:58ZengMDPI AGPlants2223-77472025-01-0114225910.3390/plants14020259Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i>Amit Raj Sharma0Gaurav Gajurel1Salma Abdel-Karim2Mohammad Abrar Alam3Robert Colquhoun Shields4Fabricio Medina-Bolivar5Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USAArkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USAArkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USADepartment of Chemistry and Physics, Arkansas State University, Jonesboro, AR 72401, USADepartment of Biological Sciences, Arkansas State University, Jonesboro, AR 72401, USAArkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USAPhenolic compounds, such as stilbenes and flavonoids, from <i>Dalea</i> spp. exhibit diverse biological activities, including antimicrobial, anti-inflammatory, and cytotoxicity properties. To this end, the objectives of this study were to establish hairy root cultures of <i>D. purpurea</i> and assess its capacity to produce these bioactive compounds. The co-treatment of the hairy root cultures with the elicitors methyl-β-cyclodextrin, magnesium chloride, methyl jasmonate, and hydrogen peroxide led to the production and secretion of the geranylated flavanone malheuran A, which was confirmed by mass spectrometry and NMR spectroscopy analyses. The highest amount (104.3 ± 6.9 mg/L) of malheuran A was detected at 192 h after elicitor treatment. The elicited hairy roots were re-elicited for 192 h with the same combination of elicitors, and they produced a substantial amount of malheuran A (91.9 ± 6.8 mg/L). Malheuran A, purified from extracts of the hairy root culture medium, showed potent antimicrobial activity against Gram-positive bacteria, including methicillin-resistant <i>Staphylococcus aureus</i> and vancomycin-resistant Enterococci. It also demonstrated anti-inflammatory effects by suppressing nitrite production in LPS-stimulated RAW 264.7 macrophages. These findings show that various elicitor treatments can boost malheuran A production in hairy root cultures, making them a sustainable bioproduction platform for this bioactive specialized metabolite.https://www.mdpi.com/2223-7747/14/2/259hairy root cultures<i>Dalea purpurea</i>elicitationre-elicitationmalheuran Ageranylated flavonoid |
| spellingShingle | Amit Raj Sharma Gaurav Gajurel Salma Abdel-Karim Mohammad Abrar Alam Robert Colquhoun Shields Fabricio Medina-Bolivar Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i> Plants hairy root cultures <i>Dalea purpurea</i> elicitation re-elicitation malheuran A geranylated flavonoid |
| title | Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i> |
| title_full | Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i> |
| title_fullStr | Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i> |
| title_full_unstemmed | Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i> |
| title_short | Production of Malheuran A, a Geranylated Flavonoid with Antimicrobial and Anti-Inflammatory Activities, in Hairy Root Cultures of <i>Dalea purpurea</i> |
| title_sort | production of malheuran a a geranylated flavonoid with antimicrobial and anti inflammatory activities in hairy root cultures of i dalea purpurea i |
| topic | hairy root cultures <i>Dalea purpurea</i> elicitation re-elicitation malheuran A geranylated flavonoid |
| url | https://www.mdpi.com/2223-7747/14/2/259 |
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