In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.)
The effects of factors including seedling age, different combinations of BA and NAA concentrations, Agar concentration, AgNO<sub>3</sub> and GA<sub>3</sub> on bud induction from cotyledon are analysed. The results indicate that the explants from 5-9 days old seedling are the...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2000-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.06.0588 |
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| author | XIE Jian-kun CUI Hai-rui SHU Qing-yao XIA Ying-wu SHOU Sen-yan |
| author_facet | XIE Jian-kun CUI Hai-rui SHU Qing-yao XIA Ying-wu SHOU Sen-yan |
| author_sort | XIE Jian-kun |
| collection | DOAJ |
| description | The effects of factors including seedling age, different combinations of BA and NAA concentrations, Agar concentration, AgNO<sub>3</sub> and GA<sub>3</sub> on bud induction from cotyledon are analysed. The results indicate that the explants from 5-9 days old seedling are the most efficient to induce adventious buds. The concentration of BA is the key factor to adventious buds differentiation. BA lower than 2 mg/L, is harder to induce adventious buds. The combinations of 6 mg/L BA and 0.05 mg/L NAA can improve adventious buds induction. The culture medium with 12 g/L Agar, 5 mg/L AgNO<sub>3</sub> and 2-3 mg/L GA<sub>3</sub> can increase the frequence of differentiation. According to these results, a procedure for induction of adventious buds and regeneration system for Chinese cabbage is estabished. By using this system, high regeneration frequence can be obtained, but could be affected by genotypes. |
| format | Article |
| id | doaj-art-566a8799afe94b87a4ef1b4df2b10db3 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2000-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-566a8799afe94b87a4ef1b4df2b10db32025-08-20T02:47:42ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552000-11-012658859210.3785/1008-9209.2000.06.058810089209In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.)XIE Jian-kunCUI Hai-ruiSHU Qing-yaoXIA Ying-wuSHOU Sen-yanThe effects of factors including seedling age, different combinations of BA and NAA concentrations, Agar concentration, AgNO<sub>3</sub> and GA<sub>3</sub> on bud induction from cotyledon are analysed. The results indicate that the explants from 5-9 days old seedling are the most efficient to induce adventious buds. The concentration of BA is the key factor to adventious buds differentiation. BA lower than 2 mg/L, is harder to induce adventious buds. The combinations of 6 mg/L BA and 0.05 mg/L NAA can improve adventious buds induction. The culture medium with 12 g/L Agar, 5 mg/L AgNO<sub>3</sub> and 2-3 mg/L GA<sub>3</sub> can increase the frequence of differentiation. According to these results, a procedure for induction of adventious buds and regeneration system for Chinese cabbage is estabished. By using this system, high regeneration frequence can be obtained, but could be affected by genotypes.https://www.academax.com/doi/10.3785/1008-9209.2000.06.0588<italic>Brassica campestris</italic> ssp. <italic>chinensis</italic> Lcotyledonregeneration system |
| spellingShingle | XIE Jian-kun CUI Hai-rui SHU Qing-yao XIA Ying-wu SHOU Sen-yan In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.) 浙江大学学报. 农业与生命科学版 <italic>Brassica campestris</italic> ssp. <italic>chinensis</italic> L cotyledon regeneration system |
| title | In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.) |
| title_full | In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.) |
| title_fullStr | In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.) |
| title_full_unstemmed | In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.) |
| title_short | In vitro plant regeneration system from cotyledon of Chinese cabbage (Brassica campestris ssp. chinensis L.) |
| title_sort | in vitro plant regeneration system from cotyledon of chinese cabbage brassica campestris ssp chinensis l |
| topic | <italic>Brassica campestris</italic> ssp. <italic>chinensis</italic> L cotyledon regeneration system |
| url | https://www.academax.com/doi/10.3785/1008-9209.2000.06.0588 |
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