Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci
<b>Background:</b> Antibiotic-resistant bacteria, especially <i>Staphylococcus</i> species, are a growing concern in healthcare settings and infections caused by multidrug-resistant strains are difficult to treat. Therefore, it is imperative to explore new treatment methods f...
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2025-06-01
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| author | Małgorzata Kępa Maria Miklasińska-Majdanik Aleksandra Haczyk Arkadiusz Matuła Robert D. Wojtyczka |
| author_facet | Małgorzata Kępa Maria Miklasińska-Majdanik Aleksandra Haczyk Arkadiusz Matuła Robert D. Wojtyczka |
| author_sort | Małgorzata Kępa |
| collection | DOAJ |
| description | <b>Background:</b> Antibiotic-resistant bacteria, especially <i>Staphylococcus</i> species, are a growing concern in healthcare settings and infections caused by multidrug-resistant strains are difficult to treat. Therefore, it is imperative to explore new treatment methods for these infections such as combinations of natural compounds with antibiotics. <b>Methods:</b> The main objective of this study was to investigate the antimicrobial activity of caffeic acid against staphylococcal strains. The viability of bacterial cells and half maximal inhibitory concentration (IC<sub>50</sub>) for caffeic acid were also examined. The minimum inhibitory concentration (MIC) of the caffeic acid was determined using a serial microdilution method. To study the combined effect of caffeic acid and erythromycin, the fractional inhibitory concentrations (FICs) were determined. <b>Results:</b> Caffeic acid inhibited the growth of all the tested isolates, with MIC values ranging from 256 to 1024 µg/mL and reduced bacterial cell viability at concentrations corresponding to MIC values. Caffeic acid and erythromycin showed a synergistic effect when used together against three examined strains and had an additive effect against two isolates. However, their combination was indifferent against the seven remaining staphylococci tested. <b>Conclusions:</b> The results of our research demonstrate that caffeic acid has antimicrobial properties against the tested strains. |
| format | Article |
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| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-565d1c1e598d4e719127b8bb770bca4a2025-08-20T03:32:33ZengMDPI AGPharmaceuticals1424-82472025-06-0118796410.3390/ph18070964Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on StaphylococciMałgorzata Kępa0Maria Miklasińska-Majdanik1Aleksandra Haczyk2Arkadiusz Matuła3Robert D. Wojtyczka4Department of Microbiology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, PolandDepartment of Microbiology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, PolandDepartment of Microbiology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, PolandDepartment of Microbiology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, PolandDepartment of Microbiology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, Poland<b>Background:</b> Antibiotic-resistant bacteria, especially <i>Staphylococcus</i> species, are a growing concern in healthcare settings and infections caused by multidrug-resistant strains are difficult to treat. Therefore, it is imperative to explore new treatment methods for these infections such as combinations of natural compounds with antibiotics. <b>Methods:</b> The main objective of this study was to investigate the antimicrobial activity of caffeic acid against staphylococcal strains. The viability of bacterial cells and half maximal inhibitory concentration (IC<sub>50</sub>) for caffeic acid were also examined. The minimum inhibitory concentration (MIC) of the caffeic acid was determined using a serial microdilution method. To study the combined effect of caffeic acid and erythromycin, the fractional inhibitory concentrations (FICs) were determined. <b>Results:</b> Caffeic acid inhibited the growth of all the tested isolates, with MIC values ranging from 256 to 1024 µg/mL and reduced bacterial cell viability at concentrations corresponding to MIC values. Caffeic acid and erythromycin showed a synergistic effect when used together against three examined strains and had an additive effect against two isolates. However, their combination was indifferent against the seven remaining staphylococci tested. <b>Conclusions:</b> The results of our research demonstrate that caffeic acid has antimicrobial properties against the tested strains.https://www.mdpi.com/1424-8247/18/7/964natural compoundsfractional inhibitory concentrationmultidrug-resistant strainshalf maximal inhibitory concentration |
| spellingShingle | Małgorzata Kępa Maria Miklasińska-Majdanik Aleksandra Haczyk Arkadiusz Matuła Robert D. Wojtyczka Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci Pharmaceuticals natural compounds fractional inhibitory concentration multidrug-resistant strains half maximal inhibitory concentration |
| title | Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci |
| title_full | Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci |
| title_fullStr | Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci |
| title_full_unstemmed | Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci |
| title_short | Caffeic Acid and Erythromycin: Antibacterial and Synergistic Effects on Staphylococci |
| title_sort | caffeic acid and erythromycin antibacterial and synergistic effects on staphylococci |
| topic | natural compounds fractional inhibitory concentration multidrug-resistant strains half maximal inhibitory concentration |
| url | https://www.mdpi.com/1424-8247/18/7/964 |
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