Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel
IntroductionLymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. Th...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1577206/full |
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| author | Ainsley R. Davies Ainsley R. Davies Kristy Kwong Kristy Kwong Kristy Kwong Zhijia Yu Koula E. M. Diamand Koula E. M. Diamand Fei-Ju Li Fei-Ju Li Laurensia Kannitha Sidra A. Ali Abolfazl Amjadipour Ann-Maree Padarin Michael Devoy Harpreet Vohra Bahar Miraghazadeh Simon H. Jiang Simon H. Jiang Anne Brüstle Nicolas Cherbuin Christopher J. Nolan Christopher J. Nolan Matthew C. Cook Elizabeth E. Gardiner Stuart Read Stuart Read Euan McNaughton Katrina L. Randall Katrina L. Randall Katrina L. Randall |
| author_facet | Ainsley R. Davies Ainsley R. Davies Kristy Kwong Kristy Kwong Kristy Kwong Zhijia Yu Koula E. M. Diamand Koula E. M. Diamand Fei-Ju Li Fei-Ju Li Laurensia Kannitha Sidra A. Ali Abolfazl Amjadipour Ann-Maree Padarin Michael Devoy Harpreet Vohra Bahar Miraghazadeh Simon H. Jiang Simon H. Jiang Anne Brüstle Nicolas Cherbuin Christopher J. Nolan Christopher J. Nolan Matthew C. Cook Elizabeth E. Gardiner Stuart Read Stuart Read Euan McNaughton Katrina L. Randall Katrina L. Randall Katrina L. Randall |
| author_sort | Ainsley R. Davies |
| collection | DOAJ |
| description | IntroductionLymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. The purpose of our study was to measure many lymphocyte and monocyte populations from a single sample, with the long-term aim of validating our assay for diagnostic use in the Australian regulatory environment.MethodsWe designed and optimised a novel 30-colour lymphocyte immunophenotyping panel tailored for use on a 3-laser (V-B-R) spectral flow cytometer. This panel measures over 50 lymphocyte and monocyte populations.ResultsIn this report we present data derived from 148 healthy individuals.DiscussionThis lays the groundwork for future clinical application of spectral flow cytometry tests and offers a more comprehensive approach to lymphocyte and monocyte analysis with future implications for disease diagnosis and monitoring. |
| format | Article |
| id | doaj-art-5659ad155f2f45db8c227b210c0d48ef |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-5659ad155f2f45db8c227b210c0d48ef2025-08-20T02:50:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-07-011610.3389/fimmu.2025.15772061577206Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panelAinsley R. Davies0Ainsley R. Davies1Kristy Kwong2Kristy Kwong3Kristy Kwong4Zhijia Yu5Koula E. M. Diamand6Koula E. M. Diamand7Fei-Ju Li8Fei-Ju Li9Laurensia Kannitha10Sidra A. Ali11Abolfazl Amjadipour12Ann-Maree Padarin13Michael Devoy14Harpreet Vohra15Bahar Miraghazadeh16Simon H. Jiang17Simon H. Jiang18Anne Brüstle19Nicolas Cherbuin20Christopher J. Nolan21Christopher J. Nolan22Matthew C. Cook23Elizabeth E. Gardiner24Stuart Read25Stuart Read26Euan McNaughton27Katrina L. Randall28Katrina L. Randall29Katrina L. Randall30Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, AustraliaCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, AustraliaDepartment of Medicine, University of Cambridge, Cambridge, United KingdomCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, AustraliaANU Centre for Therapeutic Discovery, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, AustraliaPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, AustraliaCytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaDivision of Genome Science and Cancer, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaCytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaCytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaDepartment of Renal Medicine, The Canberra Hospital, Canberra, ACT, AustraliaDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaNational Centre for Epidemiology and Population Health, Australian National University, Canberra, ACT, AustraliaDivision of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia0School of Medicine and Psychology, The Australian National University, Canberra, ACT, AustraliaDepartment of Medicine, University of Cambridge, Cambridge, United KingdomDivision of Genome Science and Cancer, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaPhenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia1South Australian Health and Medical Research Institute, Adelaide, SA, AustraliaCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, AustraliaCanberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia0School of Medicine and Psychology, The Australian National University, Canberra, ACT, Australia2Department of Immunology, and Department of Immunopathology, ACT Pathology, Canberra Health Services, Canberra, ACT, AustraliaIntroductionLymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. The purpose of our study was to measure many lymphocyte and monocyte populations from a single sample, with the long-term aim of validating our assay for diagnostic use in the Australian regulatory environment.MethodsWe designed and optimised a novel 30-colour lymphocyte immunophenotyping panel tailored for use on a 3-laser (V-B-R) spectral flow cytometer. This panel measures over 50 lymphocyte and monocyte populations.ResultsIn this report we present data derived from 148 healthy individuals.DiscussionThis lays the groundwork for future clinical application of spectral flow cytometry tests and offers a more comprehensive approach to lymphocyte and monocyte analysis with future implications for disease diagnosis and monitoring.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1577206/fullspectral flow cytometrylymphocytePBMCimmunophenotypingautoimmunityT cell |
| spellingShingle | Ainsley R. Davies Ainsley R. Davies Kristy Kwong Kristy Kwong Kristy Kwong Zhijia Yu Koula E. M. Diamand Koula E. M. Diamand Fei-Ju Li Fei-Ju Li Laurensia Kannitha Sidra A. Ali Abolfazl Amjadipour Ann-Maree Padarin Michael Devoy Harpreet Vohra Bahar Miraghazadeh Simon H. Jiang Simon H. Jiang Anne Brüstle Nicolas Cherbuin Christopher J. Nolan Christopher J. Nolan Matthew C. Cook Elizabeth E. Gardiner Stuart Read Stuart Read Euan McNaughton Katrina L. Randall Katrina L. Randall Katrina L. Randall Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel Frontiers in Immunology spectral flow cytometry lymphocyte PBMC immunophenotyping autoimmunity T cell |
| title | Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel |
| title_full | Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel |
| title_fullStr | Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel |
| title_full_unstemmed | Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel |
| title_short | Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel |
| title_sort | advanced immunophenotyping of lymphocyte and monocyte subsets in healthy australian adults using a novel spectral flow cytometry panel |
| topic | spectral flow cytometry lymphocyte PBMC immunophenotyping autoimmunity T cell |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1577206/full |
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