Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis
Malondialdehyde (MDA) content is a primary indicator evaluating lipid oxidation level in oil-containing foods. However, total MDA compounds (tMDA) exist in both free MDA (fMDA) and bound MDA (bMDA) forms at the stage of lipid peroxidation. The traditional spectrophotometric assay quantifies the MDA...
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| Language: | English |
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Tsinghua University Press
2024-09-01
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| Series: | Food Science of Animal Products |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSAP.2024.9240073 |
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| author | Wenjie Wang Zhiwen Zhang Zhigang Ke Yuting Ding Xuxia Zhou |
| author_facet | Wenjie Wang Zhiwen Zhang Zhigang Ke Yuting Ding Xuxia Zhou |
| author_sort | Wenjie Wang |
| collection | DOAJ |
| description | Malondialdehyde (MDA) content is a primary indicator evaluating lipid oxidation level in oil-containing foods. However, total MDA compounds (tMDA) exist in both free MDA (fMDA) and bound MDA (bMDA) forms at the stage of lipid peroxidation. The traditional spectrophotometric assay quantifies the MDA after an acid hydrolysis process, only releasing limited levels of fMDA from bMDA, which named as rMDA. Considering lack of study about the effect of hydrolysis treatments on the efficiency and result accuracy in terms of MDA detection for fish products. In this study, an alkaline hydrolysis method was compared with the conventional trichloroacetic acid (TCA) hydrolysis. The results showed that the highest MDA content was obtained under hydrolysis of surimi samples with 1 mol/L NaOH for 60 min at 60 °C, which was about 2 times of that obtained from 15 g/100 mL TCA for 30 min. The alkaline hydrolysis was proved to produce more rMDA, exhibiting higher hydrolysis efficiency over the conventional method. Present works also optimized a protocol on derivatization and liquid-liquid extraction process prior to gas chromatography-mass spectrometry quantification. The hydrolysate was firstly regulated by HCl, allowing protein to fully precipitated by the following addition of TCA. This developed liquid-liquid extraction could reduce interference from presence of protein in the aqueous phase, achieving a more sensitive result with improved precision and recovery. |
| format | Article |
| id | doaj-art-5653886f249043b7807ddc3dee022e2f |
| institution | OA Journals |
| issn | 2958-4124 2958-3780 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
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| series | Food Science of Animal Products |
| spelling | doaj-art-5653886f249043b7807ddc3dee022e2f2025-08-20T01:54:11ZengTsinghua University PressFood Science of Animal Products2958-41242958-37802024-09-0123924007310.26599/FSAP.2024.9240073Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysisWenjie Wang0Zhiwen Zhang1Zhigang Ke2Yuting Ding3Xuxia Zhou4Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaKey Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaKey Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaKey Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaKey Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaMalondialdehyde (MDA) content is a primary indicator evaluating lipid oxidation level in oil-containing foods. However, total MDA compounds (tMDA) exist in both free MDA (fMDA) and bound MDA (bMDA) forms at the stage of lipid peroxidation. The traditional spectrophotometric assay quantifies the MDA after an acid hydrolysis process, only releasing limited levels of fMDA from bMDA, which named as rMDA. Considering lack of study about the effect of hydrolysis treatments on the efficiency and result accuracy in terms of MDA detection for fish products. In this study, an alkaline hydrolysis method was compared with the conventional trichloroacetic acid (TCA) hydrolysis. The results showed that the highest MDA content was obtained under hydrolysis of surimi samples with 1 mol/L NaOH for 60 min at 60 °C, which was about 2 times of that obtained from 15 g/100 mL TCA for 30 min. The alkaline hydrolysis was proved to produce more rMDA, exhibiting higher hydrolysis efficiency over the conventional method. Present works also optimized a protocol on derivatization and liquid-liquid extraction process prior to gas chromatography-mass spectrometry quantification. The hydrolysate was firstly regulated by HCl, allowing protein to fully precipitated by the following addition of TCA. This developed liquid-liquid extraction could reduce interference from presence of protein in the aqueous phase, achieving a more sensitive result with improved precision and recovery.https://www.sciopen.com/article/10.26599/FSAP.2024.9240073malondialdehydegas chromatography-mass spectrometrylipid peroxidationhydrolysis efficiencyacetonitrile extractionanalytical protocol |
| spellingShingle | Wenjie Wang Zhiwen Zhang Zhigang Ke Yuting Ding Xuxia Zhou Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis Food Science of Animal Products malondialdehyde gas chromatography-mass spectrometry lipid peroxidation hydrolysis efficiency acetonitrile extraction analytical protocol |
| title | Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis |
| title_full | Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis |
| title_fullStr | Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis |
| title_full_unstemmed | Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis |
| title_short | Improved assay for malondialdehyde determination in surimi by gas chromatography-mass spectrometry after alkaline hydrolysis |
| title_sort | improved assay for malondialdehyde determination in surimi by gas chromatography mass spectrometry after alkaline hydrolysis |
| topic | malondialdehyde gas chromatography-mass spectrometry lipid peroxidation hydrolysis efficiency acetonitrile extraction analytical protocol |
| url | https://www.sciopen.com/article/10.26599/FSAP.2024.9240073 |
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