Protocol for in-plate staining of clinical peripheral blood mononuclear cell samples for high-throughput analysis via spectral flow cytometry

Protocol for in-plate staining of clinical peripheral blood mononuclear cell samples for high-throughput analysis via spectral flow cytometry

Summary: Clinical trial research relies on robust assays to monitor immune responses in patients. We present a protocol for optimization of in-plate staining for spectral flow cytometry tailored for high-throughput analysis of peripheral blood mononuclear cell (PBMC) samples from clinical research c...

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Bibliographic Details
Main Authors: Isabella Marciello, Sonya Feng, Ilana Gomez Diaz, Julia Huezo, F. Stephen Hodi, Maria Cimpean, Joanna Baginska
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Flow Cytometry
Cancer
Clinical Protocol
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001819
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