Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia
We performed nanopore-based metagenomic screening on 885 ticks collected from 6 locations in Mongolia and divided the results into 68 samples: 23 individual samples and 45 pools of 2–12 tick samples each. We detected bacterial and parasitic pathogens Anaplasma ovis, Babesia microti, Coxiella burnet...
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2024-10-01
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| Series: | Emerging Infectious Diseases |
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| Online Access: | https://wwwnc.cdc.gov/eid/article/30/14/24-0128_article |
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| author | Koray Ergunay Bazartseren Boldbaatar Brian P. Bourke Laura Caicedo-Quiroga Cynthia L. Tucker Andrew G. Letizia Nora G. Cleary Abigail G. Lilak Guugandaa Nyamdavaa Sharav Tumenjargal Michael E. von Fricken Yvonne-Marie Linton |
| author_facet | Koray Ergunay Bazartseren Boldbaatar Brian P. Bourke Laura Caicedo-Quiroga Cynthia L. Tucker Andrew G. Letizia Nora G. Cleary Abigail G. Lilak Guugandaa Nyamdavaa Sharav Tumenjargal Michael E. von Fricken Yvonne-Marie Linton |
| author_sort | Koray Ergunay |
| collection | DOAJ |
| description |
We performed nanopore-based metagenomic screening on 885 ticks collected from 6 locations in Mongolia and divided the results into 68 samples: 23 individual samples and 45 pools of 2–12 tick samples each. We detected bacterial and parasitic pathogens Anaplasma ovis, Babesia microti, Coxiella burnetii, Borrelia miyamotoi, Francisella tularensis subsp. holarctica and novicida, Spiroplasma ixodetis, Theileria equi, and Rickettsia spp., including R. raoultii, R. slovaca, and R. canadensis. We identified the viral pathogens Crimean-Congo hemorrhagic fever virus (2.9%), recently described Alongshan virus (ALSV) (2.9%), and Beiji nairovirus (5.8%). We assembled ALSV genomes, and maximum-likelihood analyses revealed clustering with viruses reported in humans and ticks from China. For ALSV, we identified surface glycoprotein markers associated with isolates from Asia viruses hosted by Ixodes persulcatus ticks. We also detected 20 virus species of unknown public health impact, including a near-complete Yanggou tick virus genome. Our findings demonstrate that nanopore sequencing can aid in detecting endemic and emerging tickborne pathogens.
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| format | Article |
| id | doaj-art-56450425810d4d20a1586aec440e2cb3 |
| institution | Kabale University |
| issn | 1080-6040 1080-6059 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj-art-56450425810d4d20a1586aec440e2cb32024-11-12T13:39:49ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592024-10-01301410511010.3201/eid3014.240128Metagenomic Nanopore Sequencing of Tickborne Pathogens, MongoliaKoray ErgunayBazartseren BoldbaatarBrian P. BourkeLaura Caicedo-QuirogaCynthia L. TuckerAndrew G. LetiziaNora G. ClearyAbigail G. LilakGuugandaa NyamdavaaSharav TumenjargalMichael E. von FrickenYvonne-Marie Linton We performed nanopore-based metagenomic screening on 885 ticks collected from 6 locations in Mongolia and divided the results into 68 samples: 23 individual samples and 45 pools of 2–12 tick samples each. We detected bacterial and parasitic pathogens Anaplasma ovis, Babesia microti, Coxiella burnetii, Borrelia miyamotoi, Francisella tularensis subsp. holarctica and novicida, Spiroplasma ixodetis, Theileria equi, and Rickettsia spp., including R. raoultii, R. slovaca, and R. canadensis. We identified the viral pathogens Crimean-Congo hemorrhagic fever virus (2.9%), recently described Alongshan virus (ALSV) (2.9%), and Beiji nairovirus (5.8%). We assembled ALSV genomes, and maximum-likelihood analyses revealed clustering with viruses reported in humans and ticks from China. For ALSV, we identified surface glycoprotein markers associated with isolates from Asia viruses hosted by Ixodes persulcatus ticks. We also detected 20 virus species of unknown public health impact, including a near-complete Yanggou tick virus genome. Our findings demonstrate that nanopore sequencing can aid in detecting endemic and emerging tickborne pathogens. https://wwwnc.cdc.gov/eid/article/30/14/24-0128_articlevector-borne infectionsbacteriaparasitesvirusestickborne illnessesmetagenomic sequencing |
| spellingShingle | Koray Ergunay Bazartseren Boldbaatar Brian P. Bourke Laura Caicedo-Quiroga Cynthia L. Tucker Andrew G. Letizia Nora G. Cleary Abigail G. Lilak Guugandaa Nyamdavaa Sharav Tumenjargal Michael E. von Fricken Yvonne-Marie Linton Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia Emerging Infectious Diseases vector-borne infections bacteria parasites viruses tickborne illnesses metagenomic sequencing |
| title | Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia |
| title_full | Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia |
| title_fullStr | Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia |
| title_full_unstemmed | Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia |
| title_short | Metagenomic Nanopore Sequencing of Tickborne Pathogens, Mongolia |
| title_sort | metagenomic nanopore sequencing of tickborne pathogens mongolia |
| topic | vector-borne infections bacteria parasites viruses tickborne illnesses metagenomic sequencing |
| url | https://wwwnc.cdc.gov/eid/article/30/14/24-0128_article |
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