Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits
Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this stu...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/4267158 |
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| author | Se-Joon Oh Hee-Young Park Kyung-Un Choi Sung-Won Choi Sung-Dong Kim Soo-Keun Kong Kyu-Sup Cho |
| author_facet | Se-Joon Oh Hee-Young Park Kyung-Un Choi Sung-Won Choi Sung-Dong Kim Soo-Keun Kong Kyu-Sup Cho |
| author_sort | Se-Joon Oh |
| collection | DOAJ |
| description | Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit. Control group was injected with normal saline. After 1 month, the resected auricles were examined histopathologically and immunohistochemically. The expression of collagen type II and transforming growth factor-β1 (TGF-β1) were analyzed by quantitative polymerase chain reaction. Histopathology showed islands of new cartilage formation at the site of the surgically induced defect in the ASC group. Furthermore, Masson’s trichrome staining and immunohistochemistry for S-100 showed numerous positive chondroblasts. The expression of collagen type II and TGF-β1 were significantly higher in the ASCs than in the control group. In conclusion, ASCs have regenerative effects on the auricular cartilage defect of the rabbit. These effects would be expected to contribute significantly to the regeneration of damaged cartilage tissue in vivo. |
| format | Article |
| id | doaj-art-5643a642d75f4801b3326e5e88ee770f |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5643a642d75f4801b3326e5e88ee770f2025-08-20T02:05:56ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/42671584267158Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in RabbitsSe-Joon Oh0Hee-Young Park1Kyung-Un Choi2Sung-Won Choi3Sung-Dong Kim4Soo-Keun Kong5Kyu-Sup Cho6Department of Otorhinolaryngology and Biomedical Research Institute, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Otorhinolaryngology and Biomedical Research Institute, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Pathology, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Otorhinolaryngology and Biomedical Research Institute, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Otorhinolaryngology and Biomedical Research Institute, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Otorhinolaryngology and Biomedical Research Institute, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Otorhinolaryngology and Biomedical Research Institute, Pusan National University School of Medicine, Pusan National University Hospital, Busan, Republic of KoreaTissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit. Control group was injected with normal saline. After 1 month, the resected auricles were examined histopathologically and immunohistochemically. The expression of collagen type II and transforming growth factor-β1 (TGF-β1) were analyzed by quantitative polymerase chain reaction. Histopathology showed islands of new cartilage formation at the site of the surgically induced defect in the ASC group. Furthermore, Masson’s trichrome staining and immunohistochemistry for S-100 showed numerous positive chondroblasts. The expression of collagen type II and TGF-β1 were significantly higher in the ASCs than in the control group. In conclusion, ASCs have regenerative effects on the auricular cartilage defect of the rabbit. These effects would be expected to contribute significantly to the regeneration of damaged cartilage tissue in vivo.http://dx.doi.org/10.1155/2018/4267158 |
| spellingShingle | Se-Joon Oh Hee-Young Park Kyung-Un Choi Sung-Won Choi Sung-Dong Kim Soo-Keun Kong Kyu-Sup Cho Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits Mediators of Inflammation |
| title | Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits |
| title_full | Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits |
| title_fullStr | Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits |
| title_full_unstemmed | Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits |
| title_short | Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits |
| title_sort | auricular cartilage regeneration with adipose derived stem cells in rabbits |
| url | http://dx.doi.org/10.1155/2018/4267158 |
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