Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens
Environmental factors affect nearly all land areas on the planet. Global warming is one of the most destructive of these factors because it has adverse effects on crop production systems. Plants are sessile organisms that have evolved complex mechanisms to cope with stress factors. Heat shock prot...
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Pontificia Universidad Javeriana
2023-09-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/36494 |
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| author | Fabio Marcelo Idrovo Espín Tiffany Cevallos Vilatuña |
| author_facet | Fabio Marcelo Idrovo Espín Tiffany Cevallos Vilatuña |
| author_sort | Fabio Marcelo Idrovo Espín |
| collection | DOAJ |
| description | Environmental factors affect nearly all land areas on the planet. Global warming is one of the most
destructive of these factors because it has adverse effects on crop production systems. Plants are sessile
organisms that have evolved complex mechanisms to cope with stress factors. Heat shock proteins
(HSPs) are one of those mechanisms. In this study, we analyzed a partial gene sequence that encodes
for HSP70 protein in Vasconcellea pubescens. We also measured the relative expression of the gene in
plantlets of Vasconcellea pubescens and performed biochemical assays under heat stress. The plantlets
were exposed to three temperatures 25° C (control), 45 °C and 55 °C (stress temperatures) for 4 hours.
The bioinformatic analysis led to the first description of a partial sequence of the HSP70 gene and its
evolutionary history in V. pubescens. We found significant differences for relative expression of the
HSP70 gene, percentage of electrolyte leakage, and proline content between plants subjected to heat
stress and those in the control group. Our results showed that V. pubescens displays thermotolerance
even under extreme temperatures. V. pubescens is a poorly studied species that may contain genes of
biotechnological interest (such as HSP70) that could be used for plant genetic modification. |
| format | Article |
| id | doaj-art-56427c30233a489191957a4e80a7882e |
| institution | DOAJ |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-56427c30233a489191957a4e80a7882e2025-08-20T03:20:19ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522023-09-0128327929810.11144/Javeriana.SC283.psaaPartial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescensFabio Marcelo Idrovo Espín0https://orcid.org/0000-0001-5493-6996Tiffany Cevallos Vilatuña1https://orcid.org/0000-0003-1940-8287Universidad de Las Américas, Av. de los Granados E12-41y Colimes esq., Quito. Ecuador. Universidad Central del Ecuador, LB-FCQ CL2-1, Francisco Viteri s/n y Gilberto Gatto Sobral, Quito. Ecuador.Universidad de Las Américas, Av. de los Granados E12-41y Colimes esq., Quito. Ecuador.Environmental factors affect nearly all land areas on the planet. Global warming is one of the most destructive of these factors because it has adverse effects on crop production systems. Plants are sessile organisms that have evolved complex mechanisms to cope with stress factors. Heat shock proteins (HSPs) are one of those mechanisms. In this study, we analyzed a partial gene sequence that encodes for HSP70 protein in Vasconcellea pubescens. We also measured the relative expression of the gene in plantlets of Vasconcellea pubescens and performed biochemical assays under heat stress. The plantlets were exposed to three temperatures 25° C (control), 45 °C and 55 °C (stress temperatures) for 4 hours. The bioinformatic analysis led to the first description of a partial sequence of the HSP70 gene and its evolutionary history in V. pubescens. We found significant differences for relative expression of the HSP70 gene, percentage of electrolyte leakage, and proline content between plants subjected to heat stress and those in the control group. Our results showed that V. pubescens displays thermotolerance even under extreme temperatures. V. pubescens is a poorly studied species that may contain genes of biotechnological interest (such as HSP70) that could be used for plant genetic modification.https://revistas.javeriana.edu.co/index.php/scientarium/article/view/36494vphsp70 gene; vasconcellea pubescens; heat stress; global warming; thermotolerance. |
| spellingShingle | Fabio Marcelo Idrovo Espín Tiffany Cevallos Vilatuña Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens Universitas Scientiarum vphsp70 gene; vasconcellea pubescens; heat stress; global warming; thermotolerance. |
| title | Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens |
| title_full | Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens |
| title_fullStr | Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens |
| title_full_unstemmed | Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens |
| title_short | Partial sequence analysis and relative expression of the HSP70 gene of Vasconcellea pubescens |
| title_sort | partial sequence analysis and relative expression of the hsp70 gene of vasconcellea pubescens |
| topic | vphsp70 gene; vasconcellea pubescens; heat stress; global warming; thermotolerance. |
| url | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/36494 |
| work_keys_str_mv | AT fabiomarceloidrovoespin partialsequenceanalysisandrelativeexpressionofthehsp70geneofvasconcelleapubescens AT tiffanycevallosvilatuna partialsequenceanalysisandrelativeexpressionofthehsp70geneofvasconcelleapubescens |