Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation
Gout is a prevalent form of aseptic inflammation caused by the deposition of monosodium urate (MSU) crystals in joints or tissues. Triggering receptor expressed on myeloid cell-1 (TREM-1) is a superimmunoglobulin receptor expressed on innate immune cells including granulocytes, monocytes, and macrop...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/5647074 |
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| author | Yonglong He Qibin Yang Xiuxiu Wang Aimin Jia Wenguang Xie Jingguo Zhou |
| author_facet | Yonglong He Qibin Yang Xiuxiu Wang Aimin Jia Wenguang Xie Jingguo Zhou |
| author_sort | Yonglong He |
| collection | DOAJ |
| description | Gout is a prevalent form of aseptic inflammation caused by the deposition of monosodium urate (MSU) crystals in joints or tissues. Triggering receptor expressed on myeloid cell-1 (TREM-1) is a superimmunoglobulin receptor expressed on innate immune cells including granulocytes, monocytes, and macrophages. TREM-1 serves as a link between innate immunity and adaptive immunity, playing a crucial role in regulating inflammation and immune response. The purpose of this study was to investigate the potential role of TREM-1 in THP-1 cells and peripheral blood mononuclear cells (PBMCs) from patients with gouty arthritis (GA). In the current study, we found that the mRNA and protein levels of TREM-1 increased in PBMCs from GA patients and soluble TREM-1 in plasma as well. In addition, an increased level of TREM-1 was observed in THP-1 treated with monosodium urate (MSU) in vitro, along with upregulation of proinflammatory cytokines. Moreover, upon specific inhibition of TREM-1, Toll-like receptor 4 (TLR-4), and myeloid differentiation factor 88 (MyD88), the levels of MyD88 and proinflammatory cytokines were decreased after MSU challenge in THP-1 cells. Interestingly, inhibition of TLR-4 could enhance the effect of TREM-1 inhibitor in MSU-induced inflammation. Taken together, our findings suggested that TREM-1 could accelerate MSU-induced acute inflammation. Inhibition of TREM-1 may provide a new strategy for alleviating acute gouty inflammation. |
| format | Article |
| id | doaj-art-563a3bcd53d94bf3b8f5adc11e326401 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-563a3bcd53d94bf3b8f5adc11e3264012025-08-20T03:20:20ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/56470745647074Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty InflammationYonglong He0Qibin Yang1Xiuxiu Wang2Aimin Jia3Wenguang Xie4Jingguo Zhou5Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, ChinaDepartment of Rheumatology and Immunology, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, ChinaDepartment of Hematology, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, ChinaDepartment of Rheumatology and Immunology, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, ChinaDepartment of Rheumatology and Immunology, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, ChinaDepartment of Rheumatology and Immunology, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, ChinaGout is a prevalent form of aseptic inflammation caused by the deposition of monosodium urate (MSU) crystals in joints or tissues. Triggering receptor expressed on myeloid cell-1 (TREM-1) is a superimmunoglobulin receptor expressed on innate immune cells including granulocytes, monocytes, and macrophages. TREM-1 serves as a link between innate immunity and adaptive immunity, playing a crucial role in regulating inflammation and immune response. The purpose of this study was to investigate the potential role of TREM-1 in THP-1 cells and peripheral blood mononuclear cells (PBMCs) from patients with gouty arthritis (GA). In the current study, we found that the mRNA and protein levels of TREM-1 increased in PBMCs from GA patients and soluble TREM-1 in plasma as well. In addition, an increased level of TREM-1 was observed in THP-1 treated with monosodium urate (MSU) in vitro, along with upregulation of proinflammatory cytokines. Moreover, upon specific inhibition of TREM-1, Toll-like receptor 4 (TLR-4), and myeloid differentiation factor 88 (MyD88), the levels of MyD88 and proinflammatory cytokines were decreased after MSU challenge in THP-1 cells. Interestingly, inhibition of TLR-4 could enhance the effect of TREM-1 inhibitor in MSU-induced inflammation. Taken together, our findings suggested that TREM-1 could accelerate MSU-induced acute inflammation. Inhibition of TREM-1 may provide a new strategy for alleviating acute gouty inflammation.http://dx.doi.org/10.1155/2019/5647074 |
| spellingShingle | Yonglong He Qibin Yang Xiuxiu Wang Aimin Jia Wenguang Xie Jingguo Zhou Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation Mediators of Inflammation |
| title | Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation |
| title_full | Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation |
| title_fullStr | Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation |
| title_full_unstemmed | Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation |
| title_short | Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation |
| title_sort | inhibition of triggering receptor expressed on myeloid cell 1 alleviates acute gouty inflammation |
| url | http://dx.doi.org/10.1155/2019/5647074 |
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