Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration
Magnesium-degradable implants have excellent mechanical and osteogenic properties for temporary orthopedic use but are underutilized due to insufficient methods to monitor implant osseointegration and tissue healing. This study evaluated the use of circulatory biomarkers to monitor the bilateral imp...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-04-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314241290595 |
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| author | Eduarda Mota-Silva Diana C. Martinez Giuseppina Basta Serena Babboni Serena del Turco Davide Fragnito Stefano Salvadori Claudia Kusmic Leon Riehakainen Daniele Panetta Beatrice Campanella Massimo Onor Tomasz Plocinski Wojciech Swieszkowski Luca Menichetti |
| author_facet | Eduarda Mota-Silva Diana C. Martinez Giuseppina Basta Serena Babboni Serena del Turco Davide Fragnito Stefano Salvadori Claudia Kusmic Leon Riehakainen Daniele Panetta Beatrice Campanella Massimo Onor Tomasz Plocinski Wojciech Swieszkowski Luca Menichetti |
| author_sort | Eduarda Mota-Silva |
| collection | DOAJ |
| description | Magnesium-degradable implants have excellent mechanical and osteogenic properties for temporary orthopedic use but are underutilized due to insufficient methods to monitor implant osseointegration and tissue healing. This study evaluated the use of circulatory biomarkers to monitor the bilateral implantation of a Mg-alloy in rats’ femurs. A total of 16 biomarkers were measured from plasma samples collected at multiple timepoints up to 90 days post-implantation. Mg-alloy, Ti-alloy, and Sham (noncritical bone defect) groups were followed with computed tomography, histological, and SEM-EDX analysis. The Sham group showed higher DKK1, OPG, VEGF, and KIM-1 levels than implanted groups. The Mg-alloy group had delayed bone regeneration due to gas release but demonstrated active regeneration up to 180 days and superior osseointegration. Elevated IL-10 and reduced FGF23 at day 28 correlated with accelerated implant degradation. These results underline the complex interactions between biomaterials and biological systems in orthopedic applications and show the value of circulating markers to follow-up implantation. |
| format | Article |
| id | doaj-art-5626585cd98d403eb5a589e2d5cb472a |
| institution | Kabale University |
| issn | 2041-7314 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj-art-5626585cd98d403eb5a589e2d5cb472a2025-08-20T03:52:16ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-04-011610.1177/20417314241290595Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regenerationEduarda Mota-Silva0Diana C. Martinez1Giuseppina Basta2Serena Babboni3Serena del Turco4Davide Fragnito5Stefano Salvadori6Claudia Kusmic7Leon Riehakainen8Daniele Panetta9Beatrice Campanella10Massimo Onor11Tomasz Plocinski12Wojciech Swieszkowski13Luca Menichetti14Institute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyBiomaterials Group, Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, PolandInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyIstituto di Chimica dei Composti Organometallici, CNR, San Cataldo Research Area, Pisa, ItalyIstituto di Chimica dei Composti Organometallici, CNR, San Cataldo Research Area, Pisa, ItalyBiomaterials Group, Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, PolandBiomaterials Group, Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, PolandInstitute of Clinical Physiology, CNR, San Cataldo Research Area, Pisa, ItalyMagnesium-degradable implants have excellent mechanical and osteogenic properties for temporary orthopedic use but are underutilized due to insufficient methods to monitor implant osseointegration and tissue healing. This study evaluated the use of circulatory biomarkers to monitor the bilateral implantation of a Mg-alloy in rats’ femurs. A total of 16 biomarkers were measured from plasma samples collected at multiple timepoints up to 90 days post-implantation. Mg-alloy, Ti-alloy, and Sham (noncritical bone defect) groups were followed with computed tomography, histological, and SEM-EDX analysis. The Sham group showed higher DKK1, OPG, VEGF, and KIM-1 levels than implanted groups. The Mg-alloy group had delayed bone regeneration due to gas release but demonstrated active regeneration up to 180 days and superior osseointegration. Elevated IL-10 and reduced FGF23 at day 28 correlated with accelerated implant degradation. These results underline the complex interactions between biomaterials and biological systems in orthopedic applications and show the value of circulating markers to follow-up implantation.https://doi.org/10.1177/20417314241290595 |
| spellingShingle | Eduarda Mota-Silva Diana C. Martinez Giuseppina Basta Serena Babboni Serena del Turco Davide Fragnito Stefano Salvadori Claudia Kusmic Leon Riehakainen Daniele Panetta Beatrice Campanella Massimo Onor Tomasz Plocinski Wojciech Swieszkowski Luca Menichetti Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration Journal of Tissue Engineering |
| title | Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration |
| title_full | Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration |
| title_fullStr | Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration |
| title_full_unstemmed | Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration |
| title_short | Monitoring osseointegration and degradation of Mg-alloy implants through plasma biomarkers of inflammation and bone regeneration |
| title_sort | monitoring osseointegration and degradation of mg alloy implants through plasma biomarkers of inflammation and bone regeneration |
| url | https://doi.org/10.1177/20417314241290595 |
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