Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells
Chitosan oligosaccharide (COS) is receiving increasing attention as a feed additive in animal production. COS has a variety of biological functions, including anti-inflammatory and antioxidant activities. Mastitis is a major disease in dairy cows that has a significant impact on animal welfare and p...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/1/31 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588057053757440 |
|---|---|
| author | Ziwei Lin Yanlong Zhou Ruiwen Chen Qiuyan Tao Qiwen Lu Qianchao Xu Haibin Yu Ping Jiang Zhihui Zhao |
| author_facet | Ziwei Lin Yanlong Zhou Ruiwen Chen Qiuyan Tao Qiwen Lu Qianchao Xu Haibin Yu Ping Jiang Zhihui Zhao |
| author_sort | Ziwei Lin |
| collection | DOAJ |
| description | Chitosan oligosaccharide (COS) is receiving increasing attention as a feed additive in animal production. COS has a variety of biological functions, including anti-inflammatory and antioxidant activities. Mastitis is a major disease in dairy cows that has a significant impact on animal welfare and production. Hence, this research aimed to investigate the mechanism of COS on the lipopolysaccharide (LPS)-stimulated inflammatory response and oxidative stress in bovine mammary epithelial cells (BMECs). In this study, the results demonstrated that COS protected BMECs from the inflammatory response induced by LPS by restraining the excessive production of toll-like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). COS treatment also suppressed excessive reactive oxygen species (ROS) production and restored antioxidant enzyme activity under LPS-induced oxidative stress conditions. Furthermore, the results also demonstrated that COS promote nuclear factor erythroid 2-related factor 2 (Nrf2) expression and inhibit TLR4 levels and p65 and IκBα phosphorylation in BMECs exposed to LPS. In summary, the results demonstrate that the protective mechanism of COS on the LPS-induced inflammatory response and oxidative stress depend on the TLR4/nuclear factor-κB (NF-κB) and Nrf2 signaling pathways, indicating that COS could serve as natural protective agents for alleviating BMECs in mastitis. |
| format | Article |
| id | doaj-art-561c5c45978a47cc8770919afb9eb266 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-561c5c45978a47cc8770919afb9eb2662025-01-24T13:39:32ZengMDPI AGMarine Drugs1660-33972025-01-012313110.3390/md23010031Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial CellsZiwei Lin0Yanlong Zhou1Ruiwen Chen2Qiuyan Tao3Qiwen Lu4Qianchao Xu5Haibin Yu6Ping Jiang7Zhihui Zhao8The Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaChitosan oligosaccharide (COS) is receiving increasing attention as a feed additive in animal production. COS has a variety of biological functions, including anti-inflammatory and antioxidant activities. Mastitis is a major disease in dairy cows that has a significant impact on animal welfare and production. Hence, this research aimed to investigate the mechanism of COS on the lipopolysaccharide (LPS)-stimulated inflammatory response and oxidative stress in bovine mammary epithelial cells (BMECs). In this study, the results demonstrated that COS protected BMECs from the inflammatory response induced by LPS by restraining the excessive production of toll-like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). COS treatment also suppressed excessive reactive oxygen species (ROS) production and restored antioxidant enzyme activity under LPS-induced oxidative stress conditions. Furthermore, the results also demonstrated that COS promote nuclear factor erythroid 2-related factor 2 (Nrf2) expression and inhibit TLR4 levels and p65 and IκBα phosphorylation in BMECs exposed to LPS. In summary, the results demonstrate that the protective mechanism of COS on the LPS-induced inflammatory response and oxidative stress depend on the TLR4/nuclear factor-κB (NF-κB) and Nrf2 signaling pathways, indicating that COS could serve as natural protective agents for alleviating BMECs in mastitis.https://www.mdpi.com/1660-3397/23/1/31chitosan oligosaccharidelipopolysaccharideTLR4/NF-κB signaling pathwayNrf2 signaling pathwaybovine mammary epithelial cells |
| spellingShingle | Ziwei Lin Yanlong Zhou Ruiwen Chen Qiuyan Tao Qiwen Lu Qianchao Xu Haibin Yu Ping Jiang Zhihui Zhao Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells Marine Drugs chitosan oligosaccharide lipopolysaccharide TLR4/NF-κB signaling pathway Nrf2 signaling pathway bovine mammary epithelial cells |
| title | Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells |
| title_full | Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells |
| title_fullStr | Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells |
| title_full_unstemmed | Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells |
| title_short | Protective Effects of Chitosan Oligosaccharide Against Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress in Bovine Mammary Epithelial Cells |
| title_sort | protective effects of chitosan oligosaccharide against lipopolysaccharide induced inflammatory response and oxidative stress in bovine mammary epithelial cells |
| topic | chitosan oligosaccharide lipopolysaccharide TLR4/NF-κB signaling pathway Nrf2 signaling pathway bovine mammary epithelial cells |
| url | https://www.mdpi.com/1660-3397/23/1/31 |
| work_keys_str_mv | AT ziweilin protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT yanlongzhou protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT ruiwenchen protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT qiuyantao protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT qiwenlu protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT qianchaoxu protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT haibinyu protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT pingjiang protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells AT zhihuizhao protectiveeffectsofchitosanoligosaccharideagainstlipopolysaccharideinducedinflammatoryresponseandoxidativestressinbovinemammaryepithelialcells |