Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus
Krill oil, as a high-quality source of phospholipids, has been shown to positively influence the gonadal development of aquatic animals. Five isonitrogenous and isolipidic diets with krill oil (KO) supplementation at 0 % (Ctrl), 1 %, 2 %, 3 %, and 4 % were formulated. A 10-week nutritional enhanceme...
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Elsevier
2025-07-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002236 |
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| author | Zongzheng Jiang Chang Xu Xiaolong Yang Fenglu Han Meili Chi Erchao Li |
| author_facet | Zongzheng Jiang Chang Xu Xiaolong Yang Fenglu Han Meili Chi Erchao Li |
| author_sort | Zongzheng Jiang |
| collection | DOAJ |
| description | Krill oil, as a high-quality source of phospholipids, has been shown to positively influence the gonadal development of aquatic animals. Five isonitrogenous and isolipidic diets with krill oil (KO) supplementation at 0 % (Ctrl), 1 %, 2 %, 3 %, and 4 % were formulated. A 10-week nutritional enhancement trial was conducted to assess the effects on ovarian development in female Cherax quadricarinatus broodstock. The result indicated that linear and quadratic increase of hepatosomatic index and gonadosomatic index by increasing KO level, and reached the peaks at 3 %. Female crayfish fed the diet with 3 % KO significantly enhanced antioxidative capacity, steroid hormone synthesis, as well as vitellogenin synthesis and transport. Based on ovarian histology and vitellogenin analysis, the results indicated that dietary 3 % KO supplementation accelerated yolk granule deposition and promoted oocyte maturation. Additionally, 3 % KO supplementation promoted lipid deposition and mobilization efficiency of triglyceride between hepatopancreas to the ovary. From serum metabolomics, a large number of phosphatidylcholine and phosphatidylethanolamine with different fatty acids were detected, which indicated vital role in ovarian maturation. The metabolism of glycerophospholipids, glycine, serine, threonine, and arachidonic acid was significantly upregulated in the 3 % KO group. Notably, the relative contents of prostaglandin E2, prostaglandin B2, and prostaglandin A2 were significantly increased. Prostaglandins are closely related to hormone secretion, yolk deposition, lipid metabolism, and antioxidant capacity, showing particularly strong correlations with estradiol, progesterone, molt-inhibiting hormone, gonad-inhibiting hormone, and ovarian vitellogenin receptor. In conclusion, adding 2.82–3.20 % krill oil to the feed promotes the healthy development of female C. quadricarinatus ovaries. This study highlights the potential of krill oil as a high-quality phospholipid source for optimizing broodstock nutrition and enhancing reproductive performance in crustacean aquaculture. |
| format | Article |
| id | doaj-art-5616ce3beb9340e3bc19e408ced0aba2 |
| institution | OA Journals |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-5616ce3beb9340e3bc19e408ced0aba22025-08-20T02:31:12ZengElsevierAquaculture Reports2352-51342025-07-014210283710.1016/j.aqrep.2025.102837Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatusZongzheng Jiang0Chang Xu1Xiaolong Yang2Fenglu Han3Meili Chi4Erchao Li5Hainan Aquaculture Breeding Engineering Research Centre, School of Marine Biology and Aquaculture, Hainan University, Haikou, Hainan 570228, ChinaHainan Aquaculture Breeding Engineering Research Centre, School of Marine Biology and Aquaculture, Hainan University, Haikou, Hainan 570228, China; Corresponding authors.Hainan Aquaculture Breeding Engineering Research Centre, School of Marine Biology and Aquaculture, Hainan University, Haikou, Hainan 570228, ChinaHainan Aquaculture Breeding Engineering Research Centre, School of Marine Biology and Aquaculture, Hainan University, Haikou, Hainan 570228, ChinaKey Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang 313001, China; Corresponding authors.School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, ChinaKrill oil, as a high-quality source of phospholipids, has been shown to positively influence the gonadal development of aquatic animals. Five isonitrogenous and isolipidic diets with krill oil (KO) supplementation at 0 % (Ctrl), 1 %, 2 %, 3 %, and 4 % were formulated. A 10-week nutritional enhancement trial was conducted to assess the effects on ovarian development in female Cherax quadricarinatus broodstock. The result indicated that linear and quadratic increase of hepatosomatic index and gonadosomatic index by increasing KO level, and reached the peaks at 3 %. Female crayfish fed the diet with 3 % KO significantly enhanced antioxidative capacity, steroid hormone synthesis, as well as vitellogenin synthesis and transport. Based on ovarian histology and vitellogenin analysis, the results indicated that dietary 3 % KO supplementation accelerated yolk granule deposition and promoted oocyte maturation. Additionally, 3 % KO supplementation promoted lipid deposition and mobilization efficiency of triglyceride between hepatopancreas to the ovary. From serum metabolomics, a large number of phosphatidylcholine and phosphatidylethanolamine with different fatty acids were detected, which indicated vital role in ovarian maturation. The metabolism of glycerophospholipids, glycine, serine, threonine, and arachidonic acid was significantly upregulated in the 3 % KO group. Notably, the relative contents of prostaglandin E2, prostaglandin B2, and prostaglandin A2 were significantly increased. Prostaglandins are closely related to hormone secretion, yolk deposition, lipid metabolism, and antioxidant capacity, showing particularly strong correlations with estradiol, progesterone, molt-inhibiting hormone, gonad-inhibiting hormone, and ovarian vitellogenin receptor. In conclusion, adding 2.82–3.20 % krill oil to the feed promotes the healthy development of female C. quadricarinatus ovaries. This study highlights the potential of krill oil as a high-quality phospholipid source for optimizing broodstock nutrition and enhancing reproductive performance in crustacean aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513425002236Cherax quadricarinatusBroodstockPhospholipids sourceReproductive hormonesGonadal development |
| spellingShingle | Zongzheng Jiang Chang Xu Xiaolong Yang Fenglu Han Meili Chi Erchao Li Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus Aquaculture Reports Cherax quadricarinatus Broodstock Phospholipids source Reproductive hormones Gonadal development |
| title | Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus |
| title_full | Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus |
| title_fullStr | Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus |
| title_full_unstemmed | Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus |
| title_short | Analysis of the optimum krill oil supplementation strategy for ovarian high-quality development in the female Redclaw crayfish, Cherax quadricarinatus |
| title_sort | analysis of the optimum krill oil supplementation strategy for ovarian high quality development in the female redclaw crayfish cherax quadricarinatus |
| topic | Cherax quadricarinatus Broodstock Phospholipids source Reproductive hormones Gonadal development |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002236 |
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