Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes
<i>Prunus mume</i> (<i>Armeniaca mume Sieb.</i> var. <i>mume f. viridicalyx</i> (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in <...
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2025-01-01
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| author | Yuxuan Liu Mengdie He Xinxin Wei Qifeng Han Xiang Yang Gangyi Huang Chen Zhang Mengqi Wu Wenqing Zhang |
| author_facet | Yuxuan Liu Mengdie He Xinxin Wei Qifeng Han Xiang Yang Gangyi Huang Chen Zhang Mengqi Wu Wenqing Zhang |
| author_sort | Yuxuan Liu |
| collection | DOAJ |
| description | <i>Prunus mume</i> (<i>Armeniaca mume Sieb.</i> var. <i>mume f. viridicalyx</i> (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in <i>Prunus mume</i> in anti-photoaging cosmetics was developed. Firstly, the flavonoids in <i>Prunus mume</i> were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the <i>Prunus mume</i> purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in <i>Prunus mume</i> as materials for anti-photoaging cosmetics. |
| format | Article |
| id | doaj-art-5611eb0ab5074618be5c28aeff4baf72 |
| institution | DOAJ |
| issn | 2079-9284 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj-art-5611eb0ab5074618be5c28aeff4baf722025-08-20T03:12:05ZengMDPI AGCosmetics2079-92842025-01-011211910.3390/cosmetics12010019Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human KeratinocytesYuxuan Liu0Mengdie He1Xinxin Wei2Qifeng Han3Xiang Yang4Gangyi Huang5Chen Zhang6Mengqi Wu7Wenqing Zhang8Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, ChinaZhejiang YingShu Biological Technology Co., Ltd., Hangzhou 311215, ChinaShanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, ChinaShanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, ChinaShanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, ChinaZhejiang YingShu Biological Technology Co., Ltd., Hangzhou 311215, ChinaZhejiang YingShu Biological Technology Co., Ltd., Hangzhou 311215, ChinaShanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, ChinaShanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China<i>Prunus mume</i> (<i>Armeniaca mume Sieb.</i> var. <i>mume f. viridicalyx</i> (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in <i>Prunus mume</i> in anti-photoaging cosmetics was developed. Firstly, the flavonoids in <i>Prunus mume</i> were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the <i>Prunus mume</i> purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in <i>Prunus mume</i> as materials for anti-photoaging cosmetics.https://www.mdpi.com/2079-9284/12/1/19<i>Prunus mume</i>extractionpurificationUHPLC-DAD-QTOF-MS/MSanti-photoaging activityhuman keratinocytes (HaCaT) |
| spellingShingle | Yuxuan Liu Mengdie He Xinxin Wei Qifeng Han Xiang Yang Gangyi Huang Chen Zhang Mengqi Wu Wenqing Zhang Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes Cosmetics <i>Prunus mume</i> extraction purification UHPLC-DAD-QTOF-MS/MS anti-photoaging activity human keratinocytes (HaCaT) |
| title | Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes |
| title_full | Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes |
| title_fullStr | Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes |
| title_full_unstemmed | Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes |
| title_short | Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes |
| title_sort | purification and identification of flavonoids from i prunus mume i and its anti photoaging activities on uvb induced human keratinocytes |
| topic | <i>Prunus mume</i> extraction purification UHPLC-DAD-QTOF-MS/MS anti-photoaging activity human keratinocytes (HaCaT) |
| url | https://www.mdpi.com/2079-9284/12/1/19 |
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