Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes

Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes

<i>Prunus mume</i> (<i>Armeniaca mume Sieb.</i> var. <i>mume f. viridicalyx</i> (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in <...

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Bibliographic Details
Main Authors: Yuxuan Liu, Mengdie He, Xinxin Wei, Qifeng Han, Xiang Yang, Gangyi Huang, Chen Zhang, Mengqi Wu, Wenqing Zhang
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Cosmetics
Subjects:
<i>Prunus mume</i>
extraction
purification
UHPLC-DAD-QTOF-MS/MS
anti-photoaging activity
human keratinocytes (HaCaT)
Online Access:https://www.mdpi.com/2079-9284/12/1/19
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Summary:<i>Prunus mume</i> (<i>Armeniaca mume Sieb.</i> var. <i>mume f. viridicalyx</i> (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in <i>Prunus mume</i> in anti-photoaging cosmetics was developed. Firstly, the flavonoids in <i>Prunus mume</i> were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the <i>Prunus mume</i> purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in <i>Prunus mume</i> as materials for anti-photoaging cosmetics.
ISSN:2079-9284

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