Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis
Objective: Rheumatoid arthritis (RA) is an autoimmune disease where sera and synovial fluid (SF) of suffering patients contain immune complexes formed from autoantibodies to several proteins. SF from humans with joint diseases was examined for the presence of some inflammatory parameters and autoant...
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Istanbul University Press
2022-12-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/61CF9E4711594B75982A7EAB3F1CF08D |
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| _version_ | 1849324312946802688 |
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| author | Nadjet Hanachi Lekhmici Arrar |
| author_facet | Nadjet Hanachi Lekhmici Arrar |
| author_sort | Nadjet Hanachi |
| collection | DOAJ |
| description | Objective: Rheumatoid arthritis (RA) is an autoimmune disease where sera and synovial fluid (SF) of suffering patients contain immune complexes formed from autoantibodies to several proteins. SF from humans with joint diseases was examined for the presence of some inflammatory parameters and autoantibodies. Materials and Methods: Antibodies in their free and complex forms were assayed by indirect ELISA. The immuno precipitation technique was used to evaluate total IgG and IgM and complement. Results: The results showed that most RA SF was anti-ASLO negative, but they were CRP positive. Levels of complement components (C3 and C4) were highest in the group of mono-/oligo-arthritis and lowest in RA. The results showed that xanthine oxidase (XO) presence and activity were important in SF of RA patients. Moreover, free and complex anti-XO antibodies were detected in all SF with different titers throughout the groups of patients where IgG was lower than IgM. Conclusion: The studied parameters of inflammation and auto-antibodies especially against XO could serve as an evaluation of the severity of joint inflammation and in RA pathogenesis understanding. |
| format | Article |
| id | doaj-art-560e964357bb443192501842ebb5bc35 |
| institution | Kabale University |
| issn | 2618-6144 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-560e964357bb443192501842ebb5bc352025-08-20T03:48:45ZengIstanbul University PressEuropean Journal of Biology2618-61442022-12-0181213614310.26650/EurJBiol.2022.1097938123456Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from ArthritisNadjet Hanachi0https://orcid.org/0000-0002-0019-3127Lekhmici Arrar1https://orcid.org/0000-0003-3822-1469University Ferhat Abbas, setif, cezayirUniversity Ferhat Abbas, setif, cezayirObjective: Rheumatoid arthritis (RA) is an autoimmune disease where sera and synovial fluid (SF) of suffering patients contain immune complexes formed from autoantibodies to several proteins. SF from humans with joint diseases was examined for the presence of some inflammatory parameters and autoantibodies. Materials and Methods: Antibodies in their free and complex forms were assayed by indirect ELISA. The immuno precipitation technique was used to evaluate total IgG and IgM and complement. Results: The results showed that most RA SF was anti-ASLO negative, but they were CRP positive. Levels of complement components (C3 and C4) were highest in the group of mono-/oligo-arthritis and lowest in RA. The results showed that xanthine oxidase (XO) presence and activity were important in SF of RA patients. Moreover, free and complex anti-XO antibodies were detected in all SF with different titers throughout the groups of patients where IgG was lower than IgM. Conclusion: The studied parameters of inflammation and auto-antibodies especially against XO could serve as an evaluation of the severity of joint inflammation and in RA pathogenesis understanding.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/61CF9E4711594B75982A7EAB3F1CF08Dautoantibodiesjoint inflammationstreptolysinrheumatoid arthritisxanthine oxidasecomplement |
| spellingShingle | Nadjet Hanachi Lekhmici Arrar Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis European Journal of Biology autoantibodies joint inflammation streptolysin rheumatoid arthritis xanthine oxidase complement |
| title | Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis |
| title_full | Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis |
| title_fullStr | Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis |
| title_full_unstemmed | Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis |
| title_short | Inflammation Parameters, Xanthine Oxidase and Anti-Xanthine Oxidase Antibodies in Synovial Fluid of Patients Suffering from Arthritis |
| title_sort | inflammation parameters xanthine oxidase and anti xanthine oxidase antibodies in synovial fluid of patients suffering from arthritis |
| topic | autoantibodies joint inflammation streptolysin rheumatoid arthritis xanthine oxidase complement |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/61CF9E4711594B75982A7EAB3F1CF08D |
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