Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial

Abstract Background Repeated implantation failure (RIF) is defined as the case whereby the transferred embryos fail to implant after several attempts of In vitro fertilization (IVF) which causes a profound impact on the quality of life and financial burden. Some clinical studies have confirmed that...

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Main Authors: Mohamed Sobhy Bakry, Elsayed Eldesouky, Moatazza Mahdy Alghazaly, Elsayed farag, Eslam Elsayed Kamal Sultan, Hossam Elazzazy, Attia Mohamed, Soliman Mohamed Said Ali, Assem Anwar, Asmaa Ahmed Elrashedy, Mohamed Abdelmonem, Mohamed Abd-ElGawad, Almandouh H. Bosilah
Format: Article
Language:English
Published: BMC 2022-11-01
Series:BMC Pregnancy and Childbirth
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Online Access:https://doi.org/10.1186/s12884-022-05098-9
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author Mohamed Sobhy Bakry
Elsayed Eldesouky
Moatazza Mahdy Alghazaly
Elsayed farag
Eslam Elsayed Kamal Sultan
Hossam Elazzazy
Attia Mohamed
Soliman Mohamed Said Ali
Assem Anwar
Asmaa Ahmed Elrashedy
Mohamed Abdelmonem
Mohamed Abd-ElGawad
Almandouh H. Bosilah
author_facet Mohamed Sobhy Bakry
Elsayed Eldesouky
Moatazza Mahdy Alghazaly
Elsayed farag
Eslam Elsayed Kamal Sultan
Hossam Elazzazy
Attia Mohamed
Soliman Mohamed Said Ali
Assem Anwar
Asmaa Ahmed Elrashedy
Mohamed Abdelmonem
Mohamed Abd-ElGawad
Almandouh H. Bosilah
author_sort Mohamed Sobhy Bakry
collection DOAJ
description Abstract Background Repeated implantation failure (RIF) is defined as the case whereby the transferred embryos fail to implant after several attempts of In vitro fertilization (IVF) which causes a profound impact on the quality of life and financial burden. Some clinical studies have confirmed that Granulocyte colony-stimulating factor (G-CSF) and human chorionic gonadotropin (HCG) can improve pregnancy outcomes and implantation rates. Hence, our study aims to compare the efficacy of G-CSF and HCG on pregnancy outcomes in RIF women who undergo intra-cytoplasmic sperm injection (ICSI). Methods This randomized, single-blinded study was conducted et al.-Azhar University Hospitals, Cairo, Egypt, between 10th October 2020 and 20th December 2020. The study included 100 women aged 20–43 years old undergoing ICSI cycles, with a history of RIF. Patients were divided randomly into two groups: group (1): included 50 patients injected with 500 IU of intrauterine HCG on embryo transfer day, and group (2): Included 50 patients injected with G-CSF on the embryo transfer day. Results In 100 RIF women, we found a significant improvement in pregnancy outcomes favoring G-CSF over HCG including implantation rate, chemical pregnancy, and clinical pregnancy (P < 0.0001, P = 0.0003, and P = 0.0006, respectively). Conclusion For the first time, we demonstrated a significant improvement in pregnancy outcomes favoring G-CSF over HCG in terms of implantation rate, chemical pregnancy, and clinical pregnancy. Trial registration The study was registered on Pan African Clinical Trials Registry with the following number: PACTR202010482774275 and was approved on 2nd October 2020.
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spelling doaj-art-55fdfc60061548f5afba4d95c8e04b292025-08-20T03:27:10ZengBMCBMC Pregnancy and Childbirth1471-23932022-11-012211910.1186/s12884-022-05098-9Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trialMohamed Sobhy Bakry0Elsayed Eldesouky1Moatazza Mahdy Alghazaly2Elsayed farag3Eslam Elsayed Kamal Sultan4Hossam Elazzazy5Attia Mohamed6Soliman Mohamed Said Ali7Assem Anwar8Asmaa Ahmed Elrashedy9Mohamed Abdelmonem10Mohamed Abd-ElGawad11Almandouh H. Bosilah12Department of Obstetrics and Gynecology, Faculty of Medicine, Fayoum UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine Girls, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Alazhar UniversityFaculty of Medicine, Kafr El-Shaikh UniversityFaculty of Medicine, Fayoum UniversityFaculty of Medicine, Fayoum UniversityDepartment of Obstetrics and Gynecology, Faculty of Medicine, Domiata UniversityAbstract Background Repeated implantation failure (RIF) is defined as the case whereby the transferred embryos fail to implant after several attempts of In vitro fertilization (IVF) which causes a profound impact on the quality of life and financial burden. Some clinical studies have confirmed that Granulocyte colony-stimulating factor (G-CSF) and human chorionic gonadotropin (HCG) can improve pregnancy outcomes and implantation rates. Hence, our study aims to compare the efficacy of G-CSF and HCG on pregnancy outcomes in RIF women who undergo intra-cytoplasmic sperm injection (ICSI). Methods This randomized, single-blinded study was conducted et al.-Azhar University Hospitals, Cairo, Egypt, between 10th October 2020 and 20th December 2020. The study included 100 women aged 20–43 years old undergoing ICSI cycles, with a history of RIF. Patients were divided randomly into two groups: group (1): included 50 patients injected with 500 IU of intrauterine HCG on embryo transfer day, and group (2): Included 50 patients injected with G-CSF on the embryo transfer day. Results In 100 RIF women, we found a significant improvement in pregnancy outcomes favoring G-CSF over HCG including implantation rate, chemical pregnancy, and clinical pregnancy (P < 0.0001, P = 0.0003, and P = 0.0006, respectively). Conclusion For the first time, we demonstrated a significant improvement in pregnancy outcomes favoring G-CSF over HCG in terms of implantation rate, chemical pregnancy, and clinical pregnancy. Trial registration The study was registered on Pan African Clinical Trials Registry with the following number: PACTR202010482774275 and was approved on 2nd October 2020.https://doi.org/10.1186/s12884-022-05098-9RIFG-CSFHCGICSIIVF
spellingShingle Mohamed Sobhy Bakry
Elsayed Eldesouky
Moatazza Mahdy Alghazaly
Elsayed farag
Eslam Elsayed Kamal Sultan
Hossam Elazzazy
Attia Mohamed
Soliman Mohamed Said Ali
Assem Anwar
Asmaa Ahmed Elrashedy
Mohamed Abdelmonem
Mohamed Abd-ElGawad
Almandouh H. Bosilah
Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial
BMC Pregnancy and Childbirth
RIF
G-CSF
HCG
ICSI
IVF
title Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial
title_full Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial
title_fullStr Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial
title_full_unstemmed Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial
title_short Granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection: a randomized clinical trial
title_sort granulocyte colony stimulating factor versus human chorionic gonadotropin for recurrent implantation failure in intra cytoplasmic sperm injection a randomized clinical trial
topic RIF
G-CSF
HCG
ICSI
IVF
url https://doi.org/10.1186/s12884-022-05098-9
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