The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses.
The sensory and physiological inputs which govern the larval-pupal transition in Drosophila, and the neuronal circuity that integrates them, are complex. Previous work from our laboratory identified a dosage-sensitive genetic interaction between the genes encoding the Rho-GEF Trio and the zinc-finge...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184950&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850076479547768832 |
|---|---|
| author | Hannah E Brown Trishna Desai Allison J Murphy Harshida Pancholi Zachary W Schmidt Hannah Swahn Eric C Liebl |
| author_facet | Hannah E Brown Trishna Desai Allison J Murphy Harshida Pancholi Zachary W Schmidt Hannah Swahn Eric C Liebl |
| author_sort | Hannah E Brown |
| collection | DOAJ |
| description | The sensory and physiological inputs which govern the larval-pupal transition in Drosophila, and the neuronal circuity that integrates them, are complex. Previous work from our laboratory identified a dosage-sensitive genetic interaction between the genes encoding the Rho-GEF Trio and the zinc-finger transcription factor Sequoia that interfered with the larval-pupal transition. Specifically, we reported heterozygous mutations in sequoia (seq) dominantly exacerbated the trio mutant phenotype, and this seq-enhanced trio mutant genotype blocked the transition of third instar larvae from foragers to wanderers, a requisite behavioral transition prior to pupation. In this work, we use the GAL4-UAS system to rescue this phenotype by tissue-specific trio expression. We find that expressing trio in the class IV dendritic arborization (da) sensory neurons rescues the larval-pupal transition, demonstrating the reliance of the larval-pupal transition on the integrity of these sensory neurons. As nociceptive responses also rely on the functionality of the class IV da neurons, we test mechanical nociceptive responses in our mutant and rescued larvae and find that mechanical nociception is separable from the ability to undergo the larval-pupal transition. This demonstrates for the first time that the roles of the class IV da neurons in governing two critical larval behaviors, the larval-pupal transition and mechanical nociception, are functionally separable from each other. |
| format | Article |
| id | doaj-art-55f4d2467f23490a9bf10cb87be81ad3 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-55f4d2467f23490a9bf10cb87be81ad32025-08-20T02:46:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018495010.1371/journal.pone.0184950The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses.Hannah E BrownTrishna DesaiAllison J MurphyHarshida PancholiZachary W SchmidtHannah SwahnEric C LieblThe sensory and physiological inputs which govern the larval-pupal transition in Drosophila, and the neuronal circuity that integrates them, are complex. Previous work from our laboratory identified a dosage-sensitive genetic interaction between the genes encoding the Rho-GEF Trio and the zinc-finger transcription factor Sequoia that interfered with the larval-pupal transition. Specifically, we reported heterozygous mutations in sequoia (seq) dominantly exacerbated the trio mutant phenotype, and this seq-enhanced trio mutant genotype blocked the transition of third instar larvae from foragers to wanderers, a requisite behavioral transition prior to pupation. In this work, we use the GAL4-UAS system to rescue this phenotype by tissue-specific trio expression. We find that expressing trio in the class IV dendritic arborization (da) sensory neurons rescues the larval-pupal transition, demonstrating the reliance of the larval-pupal transition on the integrity of these sensory neurons. As nociceptive responses also rely on the functionality of the class IV da neurons, we test mechanical nociceptive responses in our mutant and rescued larvae and find that mechanical nociception is separable from the ability to undergo the larval-pupal transition. This demonstrates for the first time that the roles of the class IV da neurons in governing two critical larval behaviors, the larval-pupal transition and mechanical nociception, are functionally separable from each other.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184950&type=printable |
| spellingShingle | Hannah E Brown Trishna Desai Allison J Murphy Harshida Pancholi Zachary W Schmidt Hannah Swahn Eric C Liebl The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses. PLoS ONE |
| title | The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses. |
| title_full | The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses. |
| title_fullStr | The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses. |
| title_full_unstemmed | The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses. |
| title_short | The function of Drosophila larval class IV dendritic arborization sensory neurons in the larval-pupal transition is separable from their function in mechanical nociception responses. |
| title_sort | function of drosophila larval class iv dendritic arborization sensory neurons in the larval pupal transition is separable from their function in mechanical nociception responses |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0184950&type=printable |
| work_keys_str_mv | AT hannahebrown thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT trishnadesai thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT allisonjmurphy thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT harshidapancholi thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT zacharywschmidt thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT hannahswahn thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT ericcliebl thefunctionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT hannahebrown functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT trishnadesai functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT allisonjmurphy functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT harshidapancholi functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT zacharywschmidt functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT hannahswahn functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses AT ericcliebl functionofdrosophilalarvalclassivdendriticarborizationsensoryneuronsinthelarvalpupaltransitionisseparablefromtheirfunctioninmechanicalnociceptionresponses |