Diffusion of Proteins Across the Nuclear Envelope of HeLa Cells
We describe an experimental system to study nucleocytoplasmic diffusion of proteins in living HeLa cells. To localize proteins to the nucleus, substrates were created that contain a nuclear localization sequence fused to Aequorea victoria green fluorescent protein (GFP). Transiently and stably trans...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98244rr04 |
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