High-throughput strategies for monoclonal antibody screening: advances and challenges
Abstract Antibodies characterized by high affinity and specificity, developed through high-throughput screening and rapid preparation, are crucial to contemporary biomedical industry. Traditional antibody preparation via the hybridoma strategy faces challenges like low efficiency, long manufacturing...
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| Format: | Article |
| Language: | English |
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BMC
2025-05-01
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| Series: | Journal of Biological Engineering |
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| Online Access: | https://doi.org/10.1186/s13036-025-00513-z |
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| author | Xiao-Dong Wang Bao-Ying Ma Shi-Ying Lai Xiang-Jing Cai Yan-Guang Cong Jun-Fa Xu Peng-Fei Zhang |
| author_facet | Xiao-Dong Wang Bao-Ying Ma Shi-Ying Lai Xiang-Jing Cai Yan-Guang Cong Jun-Fa Xu Peng-Fei Zhang |
| author_sort | Xiao-Dong Wang |
| collection | DOAJ |
| description | Abstract Antibodies characterized by high affinity and specificity, developed through high-throughput screening and rapid preparation, are crucial to contemporary biomedical industry. Traditional antibody preparation via the hybridoma strategy faces challenges like low efficiency, long manufacturing cycles, batch variability and labor intensity. Advances in molecular biology and gene editing technologies offer revolutionary improvements in antibody production. New high-throughput technologies like antibody library display, single B cell antibody technologies, and single-cell sequencing have significantly cut costs and boosted the efficiency of antibody development. These innovations accelerate commercial applications of antibodies, meeting the biopharmaceutical industry's evolving demands. This review explores recent advancements in high-throughput development of antibody, highlighting their potential advantages over traditional methods and their promising future. |
| format | Article |
| id | doaj-art-55dfcd5efbd145159e3e4db77f03bb11 |
| institution | OA Journals |
| issn | 1754-1611 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj-art-55dfcd5efbd145159e3e4db77f03bb112025-08-20T01:49:44ZengBMCJournal of Biological Engineering1754-16112025-05-0119111810.1186/s13036-025-00513-zHigh-throughput strategies for monoclonal antibody screening: advances and challengesXiao-Dong Wang0Bao-Ying Ma1Shi-Ying Lai2Xiang-Jing Cai3Yan-Guang Cong4Jun-Fa Xu5Peng-Fei Zhang6Dongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityDongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityDongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityDongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityDongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityDongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityDongguan Key Laboratory of Pathogenesis and Experimental Diagnosis of Infectious Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical UniversityAbstract Antibodies characterized by high affinity and specificity, developed through high-throughput screening and rapid preparation, are crucial to contemporary biomedical industry. Traditional antibody preparation via the hybridoma strategy faces challenges like low efficiency, long manufacturing cycles, batch variability and labor intensity. Advances in molecular biology and gene editing technologies offer revolutionary improvements in antibody production. New high-throughput technologies like antibody library display, single B cell antibody technologies, and single-cell sequencing have significantly cut costs and boosted the efficiency of antibody development. These innovations accelerate commercial applications of antibodies, meeting the biopharmaceutical industry's evolving demands. This review explores recent advancements in high-throughput development of antibody, highlighting their potential advantages over traditional methods and their promising future.https://doi.org/10.1186/s13036-025-00513-zHigh-throughputAntibody preparationDisplay system for antibody librariesSingle B cell antibodySingle-cell sequencing |
| spellingShingle | Xiao-Dong Wang Bao-Ying Ma Shi-Ying Lai Xiang-Jing Cai Yan-Guang Cong Jun-Fa Xu Peng-Fei Zhang High-throughput strategies for monoclonal antibody screening: advances and challenges Journal of Biological Engineering High-throughput Antibody preparation Display system for antibody libraries Single B cell antibody Single-cell sequencing |
| title | High-throughput strategies for monoclonal antibody screening: advances and challenges |
| title_full | High-throughput strategies for monoclonal antibody screening: advances and challenges |
| title_fullStr | High-throughput strategies for monoclonal antibody screening: advances and challenges |
| title_full_unstemmed | High-throughput strategies for monoclonal antibody screening: advances and challenges |
| title_short | High-throughput strategies for monoclonal antibody screening: advances and challenges |
| title_sort | high throughput strategies for monoclonal antibody screening advances and challenges |
| topic | High-throughput Antibody preparation Display system for antibody libraries Single B cell antibody Single-cell sequencing |
| url | https://doi.org/10.1186/s13036-025-00513-z |
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