Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass)
Abstract Background Mimosa diplotricha is an ethnic medicinal plant that has numerous ethnopharmacological applications. Here, tested the crude methanol extract (MDME), petroleum ether (MDPE), chloroform (MDCF), and n-hexane (MDNH) fractions of M. diplotricha in vivo to determine their bioactivities...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-08-01
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| Series: | Clinical Phytoscience |
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| Online Access: | https://doi.org/10.1186/s40816-025-00397-y |
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| author | Jannatul Naima Mohammad Rashedul Islam Bakul Akter Ainun Nahar Nusrat Jahan Ummah Tasnim Nisat Mohammed Kamrul Hossain |
| author_facet | Jannatul Naima Mohammad Rashedul Islam Bakul Akter Ainun Nahar Nusrat Jahan Ummah Tasnim Nisat Mohammed Kamrul Hossain |
| author_sort | Jannatul Naima |
| collection | DOAJ |
| description | Abstract Background Mimosa diplotricha is an ethnic medicinal plant that has numerous ethnopharmacological applications. Here, tested the crude methanol extract (MDME), petroleum ether (MDPE), chloroform (MDCF), and n-hexane (MDNH) fractions of M. diplotricha in vivo to determine their bioactivities, specifically CNS and oral hypoglycemic, and evaluated their antioxidant and cytotoxicity in vitro. Method Oral hypoglycemic activity was measured by an oral glucose tolerance test. Central nervous system activities were done by elevated plus maze (EPM), hole board, hole cross, and open field method. Antioxidant activity was measured by a superoxide scavenging and ferric-reducing effect assay. Cytotoxicity was determined by using a brine-shrimp lethality bioassay. Result The test samples MDME and MDME markedly reduced the blood glucose levels after 90 min of the test interval, with values of 33.58% and 39.92%, respectively. In the open-field and hole-crossed tests, all the test samples exhibited reduced locomotor activities that preceded significant sedative activity. Among them, MDNH (p < 0.001) showed a marked sedative effect. During the EPM test, mice treated with MDME and its fractions preferred to remain in the open arm, indicating antianxiety-like behavior, whereas in the hole board model, MDME extract showed a reduction in head dipping compared to standard diazepam. In the antioxidant assay, the MDME extract demonstrated a significantly lower IC50 value in the superoxide scavenging and ferric reducing assays, respectively. In the cytotoxicity assay, we observed the lowest LC50 values for each extract when compared to the standard vincristine sulfate. Conclusion The current study indicates that the plant extracts have potential antioxidant, cytotoxic, hypoglycemic, sedative, and anxiolytic activities. Graphical abstract |
| format | Article |
| id | doaj-art-55cdeb6dc43f4cf1a95ba3e0607bc2f1 |
| institution | Kabale University |
| issn | 2199-1197 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Clinical Phytoscience |
| spelling | doaj-art-55cdeb6dc43f4cf1a95ba3e0607bc2f12025-08-20T03:42:10ZengSpringerOpenClinical Phytoscience2199-11972025-08-0111111410.1186/s40816-025-00397-yPhytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass)Jannatul Naima0Mohammad Rashedul Islam1Bakul Akter2Ainun Nahar3Nusrat Jahan4Ummah Tasnim Nisat5Mohammed Kamrul Hossain6Department of Pharmacy, Faculty of Biological Sciences, University of ChittagongDepartment of Pharmacy, Faculty of Biological Sciences, University of ChittagongDepartment of Pharmacy, Faculty of Biological Sciences, University of ChittagongDepartment of Pharmacy, Faculty of Biological Sciences, University of ChittagongDepartment of Pharmacy, Faculty of Biological Sciences, University of ChittagongDepartment of Pharmacy, Faculty of Biological Sciences, University of ChittagongDepartment of Pharmacy, Faculty of Biological Sciences, University of ChittagongAbstract Background Mimosa diplotricha is an ethnic medicinal plant that has numerous ethnopharmacological applications. Here, tested the crude methanol extract (MDME), petroleum ether (MDPE), chloroform (MDCF), and n-hexane (MDNH) fractions of M. diplotricha in vivo to determine their bioactivities, specifically CNS and oral hypoglycemic, and evaluated their antioxidant and cytotoxicity in vitro. Method Oral hypoglycemic activity was measured by an oral glucose tolerance test. Central nervous system activities were done by elevated plus maze (EPM), hole board, hole cross, and open field method. Antioxidant activity was measured by a superoxide scavenging and ferric-reducing effect assay. Cytotoxicity was determined by using a brine-shrimp lethality bioassay. Result The test samples MDME and MDME markedly reduced the blood glucose levels after 90 min of the test interval, with values of 33.58% and 39.92%, respectively. In the open-field and hole-crossed tests, all the test samples exhibited reduced locomotor activities that preceded significant sedative activity. Among them, MDNH (p < 0.001) showed a marked sedative effect. During the EPM test, mice treated with MDME and its fractions preferred to remain in the open arm, indicating antianxiety-like behavior, whereas in the hole board model, MDME extract showed a reduction in head dipping compared to standard diazepam. In the antioxidant assay, the MDME extract demonstrated a significantly lower IC50 value in the superoxide scavenging and ferric reducing assays, respectively. In the cytotoxicity assay, we observed the lowest LC50 values for each extract when compared to the standard vincristine sulfate. Conclusion The current study indicates that the plant extracts have potential antioxidant, cytotoxic, hypoglycemic, sedative, and anxiolytic activities. Graphical abstracthttps://doi.org/10.1186/s40816-025-00397-yAntioxidantAnxiolyticCytotoxicityHypoglycemicSedative and phytochemical screening |
| spellingShingle | Jannatul Naima Mohammad Rashedul Islam Bakul Akter Ainun Nahar Nusrat Jahan Ummah Tasnim Nisat Mohammed Kamrul Hossain Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass) Clinical Phytoscience Antioxidant Anxiolytic Cytotoxicity Hypoglycemic Sedative and phytochemical screening |
| title | Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass) |
| title_full | Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass) |
| title_fullStr | Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass) |
| title_full_unstemmed | Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass) |
| title_short | Phytochemical and pharmacological investigation on hypoglycemia, sedation, antianxiety, free-radical scavenging, and cytotoxicity actions of Mimosa diplotricha C. Wright (Nila grass) |
| title_sort | phytochemical and pharmacological investigation on hypoglycemia sedation antianxiety free radical scavenging and cytotoxicity actions of mimosa diplotricha c wright nila grass |
| topic | Antioxidant Anxiolytic Cytotoxicity Hypoglycemic Sedative and phytochemical screening |
| url | https://doi.org/10.1186/s40816-025-00397-y |
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