Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells
Neuronal apoptosis induced by oxidative stress is a major pathological process that occurs after cerebral ischemia-reperfusion. Calycosin-7-O-β-D-glucoside (CG) is a representative component of isoflavones in Radix Astragali (RA). Previous studies have shown that CG has potential neuroprotective eff...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2019/8798069 |
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| _version_ | 1849406554719125504 |
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| author | Xiangli Yan Aiming Yu Haozhen Zheng Shengxin Wang Yingying He Lisheng Wang |
| author_facet | Xiangli Yan Aiming Yu Haozhen Zheng Shengxin Wang Yingying He Lisheng Wang |
| author_sort | Xiangli Yan |
| collection | DOAJ |
| description | Neuronal apoptosis induced by oxidative stress is a major pathological process that occurs after cerebral ischemia-reperfusion. Calycosin-7-O-β-D-glucoside (CG) is a representative component of isoflavones in Radix Astragali (RA). Previous studies have shown that CG has potential neuroprotective effects. However, whether CG alleviates neuronal apoptosis through antioxidant stress after ischemia-reperfusion remains unknown. To investigate the positive effects of CG on oxidative stress and apoptosis of neurons, we simulated the ischemia-reperfusion process in vitro using an immortalized hippocampal neuron cell line (HT22) and oxygen-glucose deprivation/reperfusion (OGD/R) model. CG significantly improved cell viability and reduced oxidative stress and neuronal apoptosis. In addition, CG treatment upregulated the expression of SIRT1, FOXO1, PGC-1α, and Bcl-2 and downregulated the expression of Bax. In summary, our findings indicate that CG alleviates OGD/R-induced damage via the SIRT1/FOXO1/PGC-1α signaling pathway. Thus, CG maybe a promising therapeutic candidate for brain injury associated with ischemic stroke. |
| format | Article |
| id | doaj-art-55cb7d68cee943ed9fe63f3684df54a4 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-55cb7d68cee943ed9fe63f3684df54a42025-08-20T03:36:19ZengWileyNeural Plasticity2090-59041687-54432019-01-01201910.1155/2019/87980698798069Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 CellsXiangli Yan0Aiming Yu1Haozhen Zheng2Shengxin Wang3Yingying He4Lisheng Wang5College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 51006 Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 51006 Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 51006 Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 51006 Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 51006 Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, 51006 Guangdong, ChinaNeuronal apoptosis induced by oxidative stress is a major pathological process that occurs after cerebral ischemia-reperfusion. Calycosin-7-O-β-D-glucoside (CG) is a representative component of isoflavones in Radix Astragali (RA). Previous studies have shown that CG has potential neuroprotective effects. However, whether CG alleviates neuronal apoptosis through antioxidant stress after ischemia-reperfusion remains unknown. To investigate the positive effects of CG on oxidative stress and apoptosis of neurons, we simulated the ischemia-reperfusion process in vitro using an immortalized hippocampal neuron cell line (HT22) and oxygen-glucose deprivation/reperfusion (OGD/R) model. CG significantly improved cell viability and reduced oxidative stress and neuronal apoptosis. In addition, CG treatment upregulated the expression of SIRT1, FOXO1, PGC-1α, and Bcl-2 and downregulated the expression of Bax. In summary, our findings indicate that CG alleviates OGD/R-induced damage via the SIRT1/FOXO1/PGC-1α signaling pathway. Thus, CG maybe a promising therapeutic candidate for brain injury associated with ischemic stroke.http://dx.doi.org/10.1155/2019/8798069 |
| spellingShingle | Xiangli Yan Aiming Yu Haozhen Zheng Shengxin Wang Yingying He Lisheng Wang Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells Neural Plasticity |
| title | Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells |
| title_full | Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells |
| title_fullStr | Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells |
| title_full_unstemmed | Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells |
| title_short | Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells |
| title_sort | calycosin 7 o β d glucoside attenuates ogd r induced damage by preventing oxidative stress and neuronal apoptosis via the sirt1 foxo1 pgc 1α pathway in ht22 cells |
| url | http://dx.doi.org/10.1155/2019/8798069 |
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