Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes
<b>Background/Objectives:</b> <i>Periploca chevalieri</i> Browicz (<i>Apocynaceae</i>), an endemic species of the Cabo Verde archipelago, is commonly used in traditional medicine for the treatment of diabetes. The aim of this study was to characterize the chemical...
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2025-06-01
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| author | Katelene Lima Maryam Malmir Shabnam Sabiha Rui Pinto Isabel Moreira da Silva Maria Eduardo Figueira João Rocha Maria Paula Duarte Olga Silva |
| author_facet | Katelene Lima Maryam Malmir Shabnam Sabiha Rui Pinto Isabel Moreira da Silva Maria Eduardo Figueira João Rocha Maria Paula Duarte Olga Silva |
| author_sort | Katelene Lima |
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| description | <b>Background/Objectives:</b> <i>Periploca chevalieri</i> Browicz (<i>Apocynaceae</i>), an endemic species of the Cabo Verde archipelago, is commonly used in traditional medicine for the treatment of diabetes. The aim of this study was to characterize the chemical profiles of the aqueous and hydroethanolic (70%) extracts of the <i>P. chevalieri</i> dried aerial parts (PcAE and PcEE) and evaluate their potential to modulate postprandial glycemia and inhibit key carbohydrate-hydrolyzing enzymes. <b>Methods:</b> The chemical characterization was performed by LC/UV-DAD-ESI/MS/MS. An in vivo evaluation of postprandial glycemia modulation was conducted on healthy CD1 mice submitted to an oral sucrose tolerance test. In vitro enzymatic inhibition was performed for the α-amylase, α-glucosidase, and DPP4 enzymes. Additionally, antioxidant and antiglycation activities were also assessed. <b>Results:</b> Phenolic acid derivatives, flavanols, proanthocyanidins, and flavonols were the major classes of secondary metabolites identified. PcEE at 170 mg/kg of body weight significantly (<i>p</i> < 0.05) reduced the postprandial glycemia peak in CD1 mice submitted to sucrose overload. Regarding the enzymatic inhibition, both extracts showed concentration-dependent inhibitory potential against the α-amylase, α-glucosidase, and DPP4 enzymes. Both extracts inhibited α-glucosidase more effectively than acarbose. <b>Conclusions:</b> The obtained results supports the traditional use of <i>P. chevalieri</i> and suggest the potential for further pharmacological investigation. |
| format | Article |
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| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj-art-55ca810119fa4d9cb5de4a7929af233e2025-08-20T03:29:45ZengMDPI AGPharmaceuticals1424-82472025-06-0118691310.3390/ph18060913Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing EnzymesKatelene Lima0Maryam Malmir1Shabnam Sabiha2Rui Pinto3Isabel Moreira da Silva4Maria Eduardo Figueira5João Rocha6Maria Paula Duarte7Olga Silva8Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, PortugalThe Mechanical Engineering and Resource Sustainability Center (MEtRICs), Chemistry Department, Nova School of Science and Technology, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal<b>Background/Objectives:</b> <i>Periploca chevalieri</i> Browicz (<i>Apocynaceae</i>), an endemic species of the Cabo Verde archipelago, is commonly used in traditional medicine for the treatment of diabetes. The aim of this study was to characterize the chemical profiles of the aqueous and hydroethanolic (70%) extracts of the <i>P. chevalieri</i> dried aerial parts (PcAE and PcEE) and evaluate their potential to modulate postprandial glycemia and inhibit key carbohydrate-hydrolyzing enzymes. <b>Methods:</b> The chemical characterization was performed by LC/UV-DAD-ESI/MS/MS. An in vivo evaluation of postprandial glycemia modulation was conducted on healthy CD1 mice submitted to an oral sucrose tolerance test. In vitro enzymatic inhibition was performed for the α-amylase, α-glucosidase, and DPP4 enzymes. Additionally, antioxidant and antiglycation activities were also assessed. <b>Results:</b> Phenolic acid derivatives, flavanols, proanthocyanidins, and flavonols were the major classes of secondary metabolites identified. PcEE at 170 mg/kg of body weight significantly (<i>p</i> < 0.05) reduced the postprandial glycemia peak in CD1 mice submitted to sucrose overload. Regarding the enzymatic inhibition, both extracts showed concentration-dependent inhibitory potential against the α-amylase, α-glucosidase, and DPP4 enzymes. Both extracts inhibited α-glucosidase more effectively than acarbose. <b>Conclusions:</b> The obtained results supports the traditional use of <i>P. chevalieri</i> and suggest the potential for further pharmacological investigation.https://www.mdpi.com/1424-8247/18/6/913<i>α</i>-amylase<i>α</i>-glucosidaseantiglycationdiabetesDPP4herbal medicine |
| spellingShingle | Katelene Lima Maryam Malmir Shabnam Sabiha Rui Pinto Isabel Moreira da Silva Maria Eduardo Figueira João Rocha Maria Paula Duarte Olga Silva Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes Pharmaceuticals <i>α</i>-amylase <i>α</i>-glucosidase antiglycation diabetes DPP4 herbal medicine |
| title | Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes |
| title_full | Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes |
| title_fullStr | Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes |
| title_full_unstemmed | Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes |
| title_short | Effects of <i>Periploca chevalieri</i> Browicz on Postprandial Glycemia and Carbohydrate-Hydrolyzing Enzymes |
| title_sort | effects of i periploca chevalieri i browicz on postprandial glycemia and carbohydrate hydrolyzing enzymes |
| topic | <i>α</i>-amylase <i>α</i>-glucosidase antiglycation diabetes DPP4 herbal medicine |
| url | https://www.mdpi.com/1424-8247/18/6/913 |
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