Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis
In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibody in the human sera. Smooth Lipopolysaccharide (LPS) was extracted from Brucella melitensis via modified hot phenol water method and fixed on the surface of the gold nanoparticles...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Kuwait Journal of Science |
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| Online Access: | https://journalskuwait.org/kjs/index.php/KJS/article/view/8581 |
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| author | Sina Vakili Gholamerza Asadikaram Masoud Torkzadeh-Mahani Alimohammad Behroozikhah Mohammad Hadi Nematollahi Amir Savardashtaki |
| author_facet | Sina Vakili Gholamerza Asadikaram Masoud Torkzadeh-Mahani Alimohammad Behroozikhah Mohammad Hadi Nematollahi Amir Savardashtaki |
| author_sort | Sina Vakili |
| collection | DOAJ |
| description |
In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibody in the human sera. Smooth Lipopolysaccharide (LPS) was extracted from Brucella melitensis via modified hot phenol water method and fixed on the surface of the gold nanoparticles by covalent interactions. In order to obtain the best performance from the designed probe, the ratio of LPS to gold nanoparticle was optimized. Dynamic light scattering was used for characterization of the probe. The reduction of the LSPR peak at 600 nm was used to quantify the amount of captured anti-Brucella antibody. Finally, satisfactory results were obtained when the nanobiosensor was used to analyze the control and patient sera for the presence of anti-Brucella antibodies.
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| format | Article |
| id | doaj-art-55c45ae903594472af6bf9da26c0ee68 |
| institution | OA Journals |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kuwait Journal of Science |
| spelling | doaj-art-55c45ae903594472af6bf9da26c0ee682025-08-20T02:03:38ZengElsevierKuwait Journal of Science2307-41082307-41162021-06-0148310.48129/kjs.v48i3.8581Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosisSina Vakili0Gholamerza Asadikaram1Masoud Torkzadeh-MahaniAlimohammad BehroozikhahMohammad Hadi NematollahiAmir SavardashtakiNeuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.kerman university of medical sciences In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibody in the human sera. Smooth Lipopolysaccharide (LPS) was extracted from Brucella melitensis via modified hot phenol water method and fixed on the surface of the gold nanoparticles by covalent interactions. In order to obtain the best performance from the designed probe, the ratio of LPS to gold nanoparticle was optimized. Dynamic light scattering was used for characterization of the probe. The reduction of the LSPR peak at 600 nm was used to quantify the amount of captured anti-Brucella antibody. Finally, satisfactory results were obtained when the nanobiosensor was used to analyze the control and patient sera for the presence of anti-Brucella antibodies. https://journalskuwait.org/kjs/index.php/KJS/article/view/8581nanobiosensorbrucellosisLSPRgold nanoparticles |
| spellingShingle | Sina Vakili Gholamerza Asadikaram Masoud Torkzadeh-Mahani Alimohammad Behroozikhah Mohammad Hadi Nematollahi Amir Savardashtaki Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis Kuwait Journal of Science nanobiosensor brucellosis LSPR gold nanoparticles |
| title | Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis |
| title_full | Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis |
| title_fullStr | Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis |
| title_full_unstemmed | Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis |
| title_short | Design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis |
| title_sort | design and construction of a localized surface plasmon resonance based gold nanobiosensor for rapid detection of brucellosis |
| topic | nanobiosensor brucellosis LSPR gold nanoparticles |
| url | https://journalskuwait.org/kjs/index.php/KJS/article/view/8581 |
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