Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway
Background: Spinal cord swelling commonly occurs following SCI. Previous studies suggest that PBM may reduce inflammation and scar formation after SCI. However, whether PBM can alleviate post-spinal cord injury edema and its underlying mechanisms have not yet been reported. This study aims to invest...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Photodiagnosis and Photodynamic Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024004010 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850245347809427456 |
|---|---|
| author | Tingyu Wu Yangguang Ma Yongyong Yang Zhihao Zhang Jie Zhou Cheng Ju Xiaoshuang Zuo Xuankang Wang Xueyu Hu Zhe Wang |
| author_facet | Tingyu Wu Yangguang Ma Yongyong Yang Zhihao Zhang Jie Zhou Cheng Ju Xiaoshuang Zuo Xuankang Wang Xueyu Hu Zhe Wang |
| author_sort | Tingyu Wu |
| collection | DOAJ |
| description | Background: Spinal cord swelling commonly occurs following SCI. Previous studies suggest that PBM may reduce inflammation and scar formation after SCI. However, whether PBM can alleviate post-spinal cord injury edema and its underlying mechanisms have not yet been reported. This study aims to investigate the effects of PBM on spinal cord swelling in rats following SCI and explore the underlying mechanisms. Methods: A rat model of SCI was established, and the rats received continuous PBM therapy for two weeks. Tissue hydration, motor function, AQP4 expression, and pathological changes in the spinal cord were evaluated at different time points. In vitro, astrocytes were subjected to PBM and treated with either cucurbitacin I or TGN020 following OGD. Results: The results indicate that PBM reduces tissue swelling in rats with SCI, improves motor function recovery, and inhibits the upregulation of AQP4 and GFAP associated with SCI. In vitro, PBM reduces abnormal activation of the JAK2/STAT3 signaling pathway in astrocytes, leading to decreased AQP4 synthesis and astrocyte activation. Conclusions: These findings suggest that PBM reduces spinal cord swelling in rats after injury. This effect is associated with the inhibition of JAK2/STAT3 signaling pathway activation in astrocytes and the reduction in AQP4 expression. |
| format | Article |
| id | doaj-art-55c42f1cef024c6f88554bca6f5ebf02 |
| institution | OA Journals |
| issn | 1572-1000 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-55c42f1cef024c6f88554bca6f5ebf022025-08-20T01:59:30ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002024-12-015010436410.1016/j.pdpdt.2024.104364Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathwayTingyu Wu0Yangguang Ma1Yongyong Yang2Zhihao Zhang3Jie Zhou4Cheng Ju5Xiaoshuang Zuo6Xuankang Wang7Xueyu Hu8Zhe Wang9Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaXi'an Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China; Corresponding authors.Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China; Corresponding authors.Background: Spinal cord swelling commonly occurs following SCI. Previous studies suggest that PBM may reduce inflammation and scar formation after SCI. However, whether PBM can alleviate post-spinal cord injury edema and its underlying mechanisms have not yet been reported. This study aims to investigate the effects of PBM on spinal cord swelling in rats following SCI and explore the underlying mechanisms. Methods: A rat model of SCI was established, and the rats received continuous PBM therapy for two weeks. Tissue hydration, motor function, AQP4 expression, and pathological changes in the spinal cord were evaluated at different time points. In vitro, astrocytes were subjected to PBM and treated with either cucurbitacin I or TGN020 following OGD. Results: The results indicate that PBM reduces tissue swelling in rats with SCI, improves motor function recovery, and inhibits the upregulation of AQP4 and GFAP associated with SCI. In vitro, PBM reduces abnormal activation of the JAK2/STAT3 signaling pathway in astrocytes, leading to decreased AQP4 synthesis and astrocyte activation. Conclusions: These findings suggest that PBM reduces spinal cord swelling in rats after injury. This effect is associated with the inhibition of JAK2/STAT3 signaling pathway activation in astrocytes and the reduction in AQP4 expression.http://www.sciencedirect.com/science/article/pii/S1572100024004010SCIEdemaAQP4Astrocytes |
| spellingShingle | Tingyu Wu Yangguang Ma Yongyong Yang Zhihao Zhang Jie Zhou Cheng Ju Xiaoshuang Zuo Xuankang Wang Xueyu Hu Zhe Wang Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway Photodiagnosis and Photodynamic Therapy SCI Edema AQP4 Astrocytes |
| title | Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway |
| title_full | Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway |
| title_fullStr | Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway |
| title_full_unstemmed | Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway |
| title_short | Photobiomodulation reduces spinal cord edema by decreasing the expression of AQP4 in the astrocytes of male spinal cord injury rats via the JAK2/STAT3 signaling pathway |
| title_sort | photobiomodulation reduces spinal cord edema by decreasing the expression of aqp4 in the astrocytes of male spinal cord injury rats via the jak2 stat3 signaling pathway |
| topic | SCI Edema AQP4 Astrocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024004010 |
| work_keys_str_mv | AT tingyuwu photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT yangguangma photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT yongyongyang photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT zhihaozhang photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT jiezhou photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT chengju photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT xiaoshuangzuo photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT xuankangwang photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT xueyuhu photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway AT zhewang photobiomodulationreducesspinalcordedemabydecreasingtheexpressionofaqp4intheastrocytesofmalespinalcordinjuryratsviathejak2stat3signalingpathway |