Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana
The leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) is a significant pest damaging various agricultural and ornamental crops. Control is hindered by its resistance to multiple insecticides. This study aimed to develop and optimize an autoinoculation device specifically for controlling L...
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Elsevier
2025-08-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425001343 |
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| author | Qikai Zhang Dengjie Wang Xinghui Liang Haihong Wang Baoqian Lyu |
| author_facet | Qikai Zhang Dengjie Wang Xinghui Liang Haihong Wang Baoqian Lyu |
| author_sort | Qikai Zhang |
| collection | DOAJ |
| description | The leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) is a significant pest damaging various agricultural and ornamental crops. Control is hindered by its resistance to multiple insecticides. This study aimed to develop and optimize an autoinoculation device specifically for controlling L. sativae and to assess the device’s efficacy in field applications. The initial phase involved screening to identify a strain of Beauveria bassiana (Balsamo-Crivelli) Vuillemin that was highly virulent to L. sativae. After selection of the optimal strain, the attractant formulation was refined and the device design was optimized to maximize pest attraction and infection rates. Short- and long-term field trials were then conducted to evaluate the device’s effectiveness in the control of pest populations. Results showed that the XJWLMQ-1 strain of B. bassiana had the highest rates of spore production, pest infection, and lethality to the targeted leafminer. The most effective attractant was a blend of trans-2-hexenal, cis-3-hexenol, 1-octen-3-ol, cis-3-hexenyl acetate, and α-ionone in a 4:1:1:3:1 ratio at 0.8 g/L concentration. Optimal efficacy was achieved by positioning the attractant 20 cm from the fungal spores and 20 cm above the cowpea canopy. Both short- and long-term trials confirmed that the autoinoculation device was effective in controlling L. sativae populations, suggesting its effective use for biological control of the target pest. |
| format | Article |
| id | doaj-art-55bf375f392e4f4d98cd82d1404cdf5e |
| institution | OA Journals |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-55bf375f392e4f4d98cd82d1404cdf5e2025-08-20T02:36:08ZengElsevierBiological Control1049-96442025-08-0120710582410.1016/j.biocontrol.2025.105824Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassianaQikai Zhang0Dengjie Wang1Xinghui Liang2Haihong Wang3Baoqian Lyu4Sanya Research Institute, CATAS/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in the Nanfan Area, Sanya, China; Environment and Plant Protection Institute, CATAS/Ministry of Agriculture and Rural Affairs Key Laboratory of Integrated Pest Management of Tropical Crops, Haikou, ChinaMianyang Academy of Agricultural Sciences, Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Key Laboratory of Wheat, Rice and Biology Breeding, Ministry of Agriculture and Rural Affairs, Mianyang, ChinaBeijing Ecoman Biotech Co. Ltd, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Corresponding authors.Sanya Research Institute, CATAS/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in the Nanfan Area, Sanya, China; Environment and Plant Protection Institute, CATAS/Ministry of Agriculture and Rural Affairs Key Laboratory of Integrated Pest Management of Tropical Crops, Haikou, China; Corresponding authors.The leafminer Liriomyza sativae Blanchard (Diptera: Agromyzidae) is a significant pest damaging various agricultural and ornamental crops. Control is hindered by its resistance to multiple insecticides. This study aimed to develop and optimize an autoinoculation device specifically for controlling L. sativae and to assess the device’s efficacy in field applications. The initial phase involved screening to identify a strain of Beauveria bassiana (Balsamo-Crivelli) Vuillemin that was highly virulent to L. sativae. After selection of the optimal strain, the attractant formulation was refined and the device design was optimized to maximize pest attraction and infection rates. Short- and long-term field trials were then conducted to evaluate the device’s effectiveness in the control of pest populations. Results showed that the XJWLMQ-1 strain of B. bassiana had the highest rates of spore production, pest infection, and lethality to the targeted leafminer. The most effective attractant was a blend of trans-2-hexenal, cis-3-hexenol, 1-octen-3-ol, cis-3-hexenyl acetate, and α-ionone in a 4:1:1:3:1 ratio at 0.8 g/L concentration. Optimal efficacy was achieved by positioning the attractant 20 cm from the fungal spores and 20 cm above the cowpea canopy. Both short- and long-term trials confirmed that the autoinoculation device was effective in controlling L. sativae populations, suggesting its effective use for biological control of the target pest.http://www.sciencedirect.com/science/article/pii/S1049964425001343Leafminer managementEntomopathogenic fungiAttractant formulationBiocontrol strategyField trial evaluation |
| spellingShingle | Qikai Zhang Dengjie Wang Xinghui Liang Haihong Wang Baoqian Lyu Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana Biological Control Leafminer management Entomopathogenic fungi Attractant formulation Biocontrol strategy Field trial evaluation |
| title | Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana |
| title_full | Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana |
| title_fullStr | Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana |
| title_full_unstemmed | Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana |
| title_short | Optimization and efficacy of an autoinoculation device for managing Liriomyza sativae with Beauveria bassiana |
| title_sort | optimization and efficacy of an autoinoculation device for managing liriomyza sativae with beauveria bassiana |
| topic | Leafminer management Entomopathogenic fungi Attractant formulation Biocontrol strategy Field trial evaluation |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425001343 |
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