Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds
The current bone autograft procedure for cleft palate repair presents several disadvantages such as limited availability, additional invasive surgery, and donor site morbidity. The present preliminary study evaluates the mineralization potential of electrospun polydioxanone:nano-hydroxyapatite : fib...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/159484 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546527737806848 |
|---|---|
| author | Isaac A. Rodriguez Parthasarathy A. Madurantakam Jennifer M. McCool Scott A. Sell Hu Yang Peter C. Moon Gary L. Bowlin |
| author_facet | Isaac A. Rodriguez Parthasarathy A. Madurantakam Jennifer M. McCool Scott A. Sell Hu Yang Peter C. Moon Gary L. Bowlin |
| author_sort | Isaac A. Rodriguez |
| collection | DOAJ |
| description | The current bone autograft procedure for cleft palate repair presents several disadvantages such as limited availability, additional invasive surgery, and donor site morbidity. The present preliminary study evaluates the mineralization potential of electrospun polydioxanone:nano-hydroxyapatite : fibrinogen (PDO : nHA : Fg) blended scaffolds in different simulated body fluids (SBF). Scaffolds were fabricated by blending PDO : nHA : Fg in the following percent by weight ratios: 100 : 0 : 0, 50 : 25 : 25, 50 : 50 : 0, 50 : 0 : 50, 0 : 0 : 100, and 0 : 50 : 50. Samples were immersed in (conventional (c), revised (r), ionic (i), and modified (m)) SBF for 5 and 14 days to induce mineralization. Scaffolds were characterized before and after mineralization via scanning electron microscopy, Alizarin Red-based assay, and modified burnout test. The addition of Fg resulted in scaffolds with smaller fiber diameters. Fg containing scaffolds also induced sheet-like mineralization while individual fiber mineralization was noticed in its absence. Mineralized electrospun Fg scaffolds without PDO were not mechanically stable after 5 days in SBF, but had superior mineralization capabilities which produced a thick bone-like mineral (BLM) layer throughout the scaffolds. 50 : 50 : 0 scaffolds incubated in either r-SBF for 5 days or c-SBF for 14 days produced scaffolds with high mineral content and individual-mineralized fibers. These mineralized scaffolds were still porous and will be further optimized as an effective bone substitute in future studies. |
| format | Article |
| id | doaj-art-55b9f6b34b09496dad3b12c16bbce7c7 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-55b9f6b34b09496dad3b12c16bbce7c72025-02-03T06:48:07ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/159484159484Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended ScaffoldsIsaac A. Rodriguez0Parthasarathy A. Madurantakam1Jennifer M. McCool2Scott A. Sell3Hu Yang4Peter C. Moon5Gary L. Bowlin6Tissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USATissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USATissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USATissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USATissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USABiomaterials Laboratory, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23298-0566, USATissue Engineering Laboratory, Department of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, East Hall, Room E1254, 401 W. Main St, P.O. Box 843067, Richmond, VA 23284-3067, USAThe current bone autograft procedure for cleft palate repair presents several disadvantages such as limited availability, additional invasive surgery, and donor site morbidity. The present preliminary study evaluates the mineralization potential of electrospun polydioxanone:nano-hydroxyapatite : fibrinogen (PDO : nHA : Fg) blended scaffolds in different simulated body fluids (SBF). Scaffolds were fabricated by blending PDO : nHA : Fg in the following percent by weight ratios: 100 : 0 : 0, 50 : 25 : 25, 50 : 50 : 0, 50 : 0 : 50, 0 : 0 : 100, and 0 : 50 : 50. Samples were immersed in (conventional (c), revised (r), ionic (i), and modified (m)) SBF for 5 and 14 days to induce mineralization. Scaffolds were characterized before and after mineralization via scanning electron microscopy, Alizarin Red-based assay, and modified burnout test. The addition of Fg resulted in scaffolds with smaller fiber diameters. Fg containing scaffolds also induced sheet-like mineralization while individual fiber mineralization was noticed in its absence. Mineralized electrospun Fg scaffolds without PDO were not mechanically stable after 5 days in SBF, but had superior mineralization capabilities which produced a thick bone-like mineral (BLM) layer throughout the scaffolds. 50 : 50 : 0 scaffolds incubated in either r-SBF for 5 days or c-SBF for 14 days produced scaffolds with high mineral content and individual-mineralized fibers. These mineralized scaffolds were still porous and will be further optimized as an effective bone substitute in future studies.http://dx.doi.org/10.1155/2012/159484 |
| spellingShingle | Isaac A. Rodriguez Parthasarathy A. Madurantakam Jennifer M. McCool Scott A. Sell Hu Yang Peter C. Moon Gary L. Bowlin Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds International Journal of Biomaterials |
| title | Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds |
| title_full | Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds |
| title_fullStr | Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds |
| title_full_unstemmed | Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds |
| title_short | Mineralization Potential of Electrospun PDO-Hydroxyapatite-Fibrinogen Blended Scaffolds |
| title_sort | mineralization potential of electrospun pdo hydroxyapatite fibrinogen blended scaffolds |
| url | http://dx.doi.org/10.1155/2012/159484 |
| work_keys_str_mv | AT isaacarodriguez mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds AT parthasarathyamadurantakam mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds AT jennifermmccool mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds AT scottasell mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds AT huyang mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds AT petercmoon mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds AT garylbowlin mineralizationpotentialofelectrospunpdohydroxyapatitefibrinogenblendedscaffolds |