Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation
Abstract Pathologists have depended on their visual experience to assess tissue structures in smear images, which was time-consuming, error-prone, and inconsistent. Deep learning, particularly Convolutional Neural Networks (CNNs), offers the ability to automate this procedure by recognizing patterns...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-90221-x |
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| author | G. V. S. Sudhamsh S. Girisha R. Rashmi |
| author_facet | G. V. S. Sudhamsh S. Girisha R. Rashmi |
| author_sort | G. V. S. Sudhamsh |
| collection | DOAJ |
| description | Abstract Pathologists have depended on their visual experience to assess tissue structures in smear images, which was time-consuming, error-prone, and inconsistent. Deep learning, particularly Convolutional Neural Networks (CNNs), offers the ability to automate this procedure by recognizing patterns in tissue images. However, training these models necessitates huge amounts of labeled data, which can be difficult to come by due to the skill required for annotation and the unavailability of data, particularly for rare diseases. This work introduces a new semi-supervised method for tissue structure semantic segmentation in histopathological images. The study presents a CNN based teacher model that generates pseudo-labels to train a student model, aiming to overcome the drawbacks of conventional supervised learning approaches. Self-supervised training is used to improve the teacher model’s performance on smaller datasets. Consistency regularization is integrated to efficiently train the student model on labeled data. Further, the study uses Monte Carlo dropout to estimate the uncertainty of proposed model. The proposed model demonstrated promising results by achieving an mIoU score of 0.64 on a public dataset, highlighting its potential to improve segmentation accuracy in histopathological image analysis. |
| format | Article |
| id | doaj-art-55b89f126d854871b78dcb01d20f4fab |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-55b89f126d854871b78dcb01d20f4fab2025-08-20T03:10:49ZengNature PortfolioScientific Reports2045-23222025-02-0115111610.1038/s41598-025-90221-xSemi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimationG. V. S. Sudhamsh0S. Girisha1R. Rashmi2Department of Computer Science and Engineering, Manipal Institute of Technology Bengaluru, Manipal Academy of Higher EducationDepartment of Data Science and Computer Applications, Manipal Institute of Technology, Manipal Academy of Higher EducationDepartment of Computer Science and Engineering, Manipal Institute of Technology Bengaluru, Manipal Academy of Higher EducationAbstract Pathologists have depended on their visual experience to assess tissue structures in smear images, which was time-consuming, error-prone, and inconsistent. Deep learning, particularly Convolutional Neural Networks (CNNs), offers the ability to automate this procedure by recognizing patterns in tissue images. However, training these models necessitates huge amounts of labeled data, which can be difficult to come by due to the skill required for annotation and the unavailability of data, particularly for rare diseases. This work introduces a new semi-supervised method for tissue structure semantic segmentation in histopathological images. The study presents a CNN based teacher model that generates pseudo-labels to train a student model, aiming to overcome the drawbacks of conventional supervised learning approaches. Self-supervised training is used to improve the teacher model’s performance on smaller datasets. Consistency regularization is integrated to efficiently train the student model on labeled data. Further, the study uses Monte Carlo dropout to estimate the uncertainty of proposed model. The proposed model demonstrated promising results by achieving an mIoU score of 0.64 on a public dataset, highlighting its potential to improve segmentation accuracy in histopathological image analysis.https://doi.org/10.1038/s41598-025-90221-xComputer Aided Diagnostic SystemsDeep LearningHistopathological Image AnalysisSemantic SegmentationSemi-Supervised Learning |
| spellingShingle | G. V. S. Sudhamsh S. Girisha R. Rashmi Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation Scientific Reports Computer Aided Diagnostic Systems Deep Learning Histopathological Image Analysis Semantic Segmentation Semi-Supervised Learning |
| title | Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation |
| title_full | Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation |
| title_fullStr | Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation |
| title_full_unstemmed | Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation |
| title_short | Semi-supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation |
| title_sort | semi supervised tissue segmentation from histopathological images with consistency regularization and uncertainty estimation |
| topic | Computer Aided Diagnostic Systems Deep Learning Histopathological Image Analysis Semantic Segmentation Semi-Supervised Learning |
| url | https://doi.org/10.1038/s41598-025-90221-x |
| work_keys_str_mv | AT gvssudhamsh semisupervisedtissuesegmentationfromhistopathologicalimageswithconsistencyregularizationanduncertaintyestimation AT sgirisha semisupervisedtissuesegmentationfromhistopathologicalimageswithconsistencyregularizationanduncertaintyestimation AT rrashmi semisupervisedtissuesegmentationfromhistopathologicalimageswithconsistencyregularizationanduncertaintyestimation |