Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger

Abstract Hospitalisation and routine antibiotic treatment are recommended for children with complicated severe acute malnutrition (SAM) but this may exacerbate antimicrobial resistance. Here, we investigate carriage of Gram-negative bacteria in children under five years of age receiving treatment fo...

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Main Authors: Kirsty Sands, Aditya Kumar Lankapalli, Giulia Lai, Brekhna Hassan, Edward AR Portal, Jordan AT Mathias, Ian Boostrom, Mei Li, Kate Cook, Shonnette Premchand-Branker, Lim S. Jones, Nathan Sayinzonga-Makombe, Sheila Isanaka, Rupa Kanapathipillai, Christopher Mambula, Isabelle Mouniaman, Céline Langendorf, Timothy R. Walsh, Owen B. Spiller
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61718-w
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author Kirsty Sands
Aditya Kumar Lankapalli
Giulia Lai
Brekhna Hassan
Edward AR Portal
Jordan AT Mathias
Ian Boostrom
Mei Li
Kate Cook
Shonnette Premchand-Branker
Lim S. Jones
Nathan Sayinzonga-Makombe
Sheila Isanaka
Rupa Kanapathipillai
Christopher Mambula
Isabelle Mouniaman
Céline Langendorf
Timothy R. Walsh
Owen B. Spiller
author_facet Kirsty Sands
Aditya Kumar Lankapalli
Giulia Lai
Brekhna Hassan
Edward AR Portal
Jordan AT Mathias
Ian Boostrom
Mei Li
Kate Cook
Shonnette Premchand-Branker
Lim S. Jones
Nathan Sayinzonga-Makombe
Sheila Isanaka
Rupa Kanapathipillai
Christopher Mambula
Isabelle Mouniaman
Céline Langendorf
Timothy R. Walsh
Owen B. Spiller
author_sort Kirsty Sands
collection DOAJ
description Abstract Hospitalisation and routine antibiotic treatment are recommended for children with complicated severe acute malnutrition (SAM) but this may exacerbate antimicrobial resistance. Here, we investigate carriage of Gram-negative bacteria in children under five years of age receiving treatment for SAM in Niger, comparing the frequency of colonisation with bacteria carrying resistance genes at admission, during hospital stay and at discharge. E. coli isolates carrying a bla NDM-5 gene were selected for whole-genome sequencing. Rectal colonisation with bacteria carrying ß-lactamase genes is high, with 76% (n = 1042/1371) of children harbouring bacteria carrying a bla CTXM-1-group gene and 25% (n = 338/1371) carrying a bla NDM-5 gene. Over two-thirds of children who did not carry bacteria with a carbapenemase gene at admission are colonised with bacteria carrying a carbapenemase gene at discharge (n = 503/729, 69%). E. coli ST167 carrying bla NDM-5 gene is recovered from 11% (n = 144/1371) of children. Here we highlight infection control and bacterial AMR transmission concerns amongst a vulnerable population in need of medical treatment.
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spelling doaj-art-55b6ca73937d4f00a30a009648ab7da42025-08-20T03:05:14ZengNature PortfolioNature Communications2041-17232025-08-0116111010.1038/s41467-025-61718-wAcquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in NigerKirsty Sands0Aditya Kumar Lankapalli1Giulia Lai2Brekhna Hassan3Edward AR Portal4Jordan AT Mathias5Ian Boostrom6Mei Li7Kate Cook8Shonnette Premchand-Branker9Lim S. Jones10Nathan Sayinzonga-Makombe11Sheila Isanaka12Rupa Kanapathipillai13Christopher Mambula14Isabelle Mouniaman15Céline Langendorf16Timothy R. Walsh17Owen B. Spiller18Ineos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordIneos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityIneos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordDivision of Infection and Immunity, Cardiff UniversityDivision of Infection and Immunity, Cardiff UniversityIneos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordIneos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordIneos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordPublic Health Wales Microbiology, University Hospital of WalesEpicentreDepartment of Research, EpicentreMédecins Sans FrontièresMédecins Sans FrontièresMédecins Sans FrontièresDepartment of Research, EpicentreIneos Oxford Institute for Antimicrobial Research, Department of Biology, University of OxfordDivision of Infection and Immunity, Cardiff UniversityAbstract Hospitalisation and routine antibiotic treatment are recommended for children with complicated severe acute malnutrition (SAM) but this may exacerbate antimicrobial resistance. Here, we investigate carriage of Gram-negative bacteria in children under five years of age receiving treatment for SAM in Niger, comparing the frequency of colonisation with bacteria carrying resistance genes at admission, during hospital stay and at discharge. E. coli isolates carrying a bla NDM-5 gene were selected for whole-genome sequencing. Rectal colonisation with bacteria carrying ß-lactamase genes is high, with 76% (n = 1042/1371) of children harbouring bacteria carrying a bla CTXM-1-group gene and 25% (n = 338/1371) carrying a bla NDM-5 gene. Over two-thirds of children who did not carry bacteria with a carbapenemase gene at admission are colonised with bacteria carrying a carbapenemase gene at discharge (n = 503/729, 69%). E. coli ST167 carrying bla NDM-5 gene is recovered from 11% (n = 144/1371) of children. Here we highlight infection control and bacterial AMR transmission concerns amongst a vulnerable population in need of medical treatment.https://doi.org/10.1038/s41467-025-61718-w
spellingShingle Kirsty Sands
Aditya Kumar Lankapalli
Giulia Lai
Brekhna Hassan
Edward AR Portal
Jordan AT Mathias
Ian Boostrom
Mei Li
Kate Cook
Shonnette Premchand-Branker
Lim S. Jones
Nathan Sayinzonga-Makombe
Sheila Isanaka
Rupa Kanapathipillai
Christopher Mambula
Isabelle Mouniaman
Céline Langendorf
Timothy R. Walsh
Owen B. Spiller
Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger
Nature Communications
title Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger
title_full Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger
title_fullStr Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger
title_full_unstemmed Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger
title_short Acquisition of Escherichia coli carrying extended-spectrum ß-lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in Niger
title_sort acquisition of escherichia coli carrying extended spectrum ss lactamase and carbapenemase genes by hospitalised children with severe acute malnutrition in niger
url https://doi.org/10.1038/s41467-025-61718-w
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