MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells
Epithelial-mesenchymal transition (EMT) is closely linked to liver cancer prognosis, invasiveness, and aggressiveness. One promising treatment for liver cancer is cell therapy, where stem cells are stimulated to develop into functional liver cells. This study aimed to investigate the effect of miR-...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2025-05-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/ejh/article/view/4190 |
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| _version_ | 1849322518983213056 |
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| author | Qianzhe Xing Yanjie Xu Ying Luo Chenglong Li Peng Wang Bin Kang Chengjun Lu |
| author_facet | Qianzhe Xing Yanjie Xu Ying Luo Chenglong Li Peng Wang Bin Kang Chengjun Lu |
| author_sort | Qianzhe Xing |
| collection | DOAJ |
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Epithelial-mesenchymal transition (EMT) is closely linked to liver cancer prognosis, invasiveness, and aggressiveness. One promising treatment for liver cancer is cell therapy, where stem cells are stimulated to develop into functional liver cells. This study aimed to investigate the effect of miR-122-5p on the differentiation of human induced pluripotent stem cells (hiPSCs) into hepatocyte-like cells and its impact on the EMT process in liver cancer cells. MiR-122-5p was overexpressed or silenced in hiPSCs to analyze the expression of liver-specific markers, including AFP, ALB and ASGPR, to confirm hepatocyte-like differentiation. A co-culture system with HepG2 liver cancer cells was also used to evaluate the effect of miR-122-5p-overexpressing hiPSCs or miR-122-5p-silencing hiPSCs on the expression of EMT markers. Results revealed that overexpression of miR-122-5p in hiPSCs induced hepatocyte-like characteristics, as evidenced by increased levels of AFP, ALB, and ASGPR. However, knockdown of miR-122-5p had the opposite effect. In the co-culture system, hiPSCs overexpressing miR-122-5p inhibited the EMT process of HepG2 cells, resulting in increased levels of mesenchymal markers and decreased levels of epithelial markers. Taken together, miR-122-5p promotes the differentiation of hiPSCs into hepatocyte-like cells and inhibits EMT process of liver cancer cells. Targeting miR-122-5p may be a novel approach to prevent liver cancer progression through cell therapy.
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| format | Article |
| id | doaj-art-5591d44c3d2e4a198071bc5baa58ca16 |
| institution | Kabale University |
| issn | 1121-760X 2038-8306 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj-art-5591d44c3d2e4a198071bc5baa58ca162025-08-20T03:49:21ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062025-05-0169210.4081/ejh.2025.4190MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cellsQianzhe Xing0Yanjie Xu1Ying Luo2Chenglong Li3Peng Wang4Bin Kang5Chengjun Lu6Department of Hepatobiliary Surgery and Tianjin Institute of Hepatobiliary Disease, Third Central Hospital of TianjinDepartment of Hepatobiliary Surgery and Tianjin Institute of Hepatobiliary Disease, Third Central Hospital of Tianjin3Tianjin Key Laboratory of Artificial Cell, Tianjin Institute of Hepatobiliary Disease, Artificial Cell Engineering Technology Research Center of Public Health Ministry, Third Central Hospital of Tianjin3Tianjin Key Laboratory of Artificial Cell, Tianjin Institute of Hepatobiliary Disease, Artificial Cell Engineering Technology Research Center of Public Health Ministry, Third Central Hospital of Tianjin3Tianjin Key Laboratory of Artificial Cell, Tianjin Institute of Hepatobiliary Disease, Artificial Cell Engineering Technology Research Center of Public Health Ministry, Third Central Hospital of TianjinDepartment of Hepatobiliary Surgery and Tianjin Institute of Hepatobiliary Disease, Third Central Hospital of TianjinDepartment of Hepatobiliary Surgery and Tianjin Institute of Hepatobiliary Disease, Third Central Hospital of Tianjin Epithelial-mesenchymal transition (EMT) is closely linked to liver cancer prognosis, invasiveness, and aggressiveness. One promising treatment for liver cancer is cell therapy, where stem cells are stimulated to develop into functional liver cells. This study aimed to investigate the effect of miR-122-5p on the differentiation of human induced pluripotent stem cells (hiPSCs) into hepatocyte-like cells and its impact on the EMT process in liver cancer cells. MiR-122-5p was overexpressed or silenced in hiPSCs to analyze the expression of liver-specific markers, including AFP, ALB and ASGPR, to confirm hepatocyte-like differentiation. A co-culture system with HepG2 liver cancer cells was also used to evaluate the effect of miR-122-5p-overexpressing hiPSCs or miR-122-5p-silencing hiPSCs on the expression of EMT markers. Results revealed that overexpression of miR-122-5p in hiPSCs induced hepatocyte-like characteristics, as evidenced by increased levels of AFP, ALB, and ASGPR. However, knockdown of miR-122-5p had the opposite effect. In the co-culture system, hiPSCs overexpressing miR-122-5p inhibited the EMT process of HepG2 cells, resulting in increased levels of mesenchymal markers and decreased levels of epithelial markers. Taken together, miR-122-5p promotes the differentiation of hiPSCs into hepatocyte-like cells and inhibits EMT process of liver cancer cells. Targeting miR-122-5p may be a novel approach to prevent liver cancer progression through cell therapy. https://www.ejh.it/ejh/article/view/4190Liver cancerinduced pluripotent stem cellsepithelial-mesenchymal transitionmiRNA |
| spellingShingle | Qianzhe Xing Yanjie Xu Ying Luo Chenglong Li Peng Wang Bin Kang Chengjun Lu MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells European Journal of Histochemistry Liver cancer induced pluripotent stem cells epithelial-mesenchymal transition miRNA |
| title | MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells |
| title_full | MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells |
| title_fullStr | MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells |
| title_full_unstemmed | MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells |
| title_short | MiR-122-5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hiPSCs to differentiate into hepatocyte-like cells |
| title_sort | mir 122 5p inhibits the epithelial mesenchymal transition of liver cancer cells by inducing hipscs to differentiate into hepatocyte like cells |
| topic | Liver cancer induced pluripotent stem cells epithelial-mesenchymal transition miRNA |
| url | https://www.ejh.it/ejh/article/view/4190 |
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