Cell Adhesion on Polycaprolactone Modified by Plasma Treatment
We have investigated the influence of various plasma treatments of electrospun polycaprolactone (PCL) scaffolds on the adhesion and proliferation of human umbilical endothelial cells (HUVEC). The PCL scaffolds were treated in plasmas created in O2, NH3 or SO2 gas at identical conditions. Surface fun...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2016/7354396 |
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| author | Nina Recek Matic Resnik Helena Motaln Tamara Lah-Turnšek Robin Augustine Nandakumar Kalarikkal Sabu Thomas Miran Mozetič |
| author_facet | Nina Recek Matic Resnik Helena Motaln Tamara Lah-Turnšek Robin Augustine Nandakumar Kalarikkal Sabu Thomas Miran Mozetič |
| author_sort | Nina Recek |
| collection | DOAJ |
| description | We have investigated the influence of various plasma treatments of electrospun polycaprolactone (PCL) scaffolds on the adhesion and proliferation of human umbilical endothelial cells (HUVEC). The PCL scaffolds were treated in plasmas created in O2, NH3 or SO2 gas at identical conditions. Surface functionalization of plasma-treated samples was determined using X-ray photoelectron spectroscopy. Cell adhesion and morphology were investigated by scanning electron microscopy and the influence of plasma treatment on cell adhesion and viability was evaluated with cell viability assay (MTT assay). The results showed the highest metabolic activity of HUVEC on PCL samples treated with O2 and NH3 plasma. Accordingly, the cells reflected the best adhesion and morphology on O2 and NH3 plasma-treated PCL samples already at 3 h. Moreover, treatment with O2 and NH3 plasma even stimulated endothelial cell proliferation on PCL surfaces by 60% as measured at 24 h, showing significant improvement in endothelialization of this material. Contrarily, SO2 plasma appeared to be less promising in comparison with O2 and NH3 plasma; however, it was still better than without any plasma treatment. Thus, our results importantly contribute to the biocompatibility improvement of the PCL polymer, commonly used for scaffolds in tissue engineering. |
| format | Article |
| id | doaj-art-558ea15d19a1414abf565e07edade4f0 |
| institution | OA Journals |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-558ea15d19a1414abf565e07edade4f02025-08-20T02:05:03ZengWileyInternational Journal of Polymer Science1687-94221687-94302016-01-01201610.1155/2016/73543967354396Cell Adhesion on Polycaprolactone Modified by Plasma TreatmentNina Recek0Matic Resnik1Helena Motaln2Tamara Lah-Turnšek3Robin Augustine4Nandakumar Kalarikkal5Sabu Thomas6Miran Mozetič7Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, SloveniaJozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, SloveniaNational Institute of Biology, Večna Pot 111, 1000 Ljubljana, SloveniaNational Institute of Biology, Večna Pot 111, 1000 Ljubljana, SloveniaInternational and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaInternational and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaInternational and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaJozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, SloveniaWe have investigated the influence of various plasma treatments of electrospun polycaprolactone (PCL) scaffolds on the adhesion and proliferation of human umbilical endothelial cells (HUVEC). The PCL scaffolds were treated in plasmas created in O2, NH3 or SO2 gas at identical conditions. Surface functionalization of plasma-treated samples was determined using X-ray photoelectron spectroscopy. Cell adhesion and morphology were investigated by scanning electron microscopy and the influence of plasma treatment on cell adhesion and viability was evaluated with cell viability assay (MTT assay). The results showed the highest metabolic activity of HUVEC on PCL samples treated with O2 and NH3 plasma. Accordingly, the cells reflected the best adhesion and morphology on O2 and NH3 plasma-treated PCL samples already at 3 h. Moreover, treatment with O2 and NH3 plasma even stimulated endothelial cell proliferation on PCL surfaces by 60% as measured at 24 h, showing significant improvement in endothelialization of this material. Contrarily, SO2 plasma appeared to be less promising in comparison with O2 and NH3 plasma; however, it was still better than without any plasma treatment. Thus, our results importantly contribute to the biocompatibility improvement of the PCL polymer, commonly used for scaffolds in tissue engineering.http://dx.doi.org/10.1155/2016/7354396 |
| spellingShingle | Nina Recek Matic Resnik Helena Motaln Tamara Lah-Turnšek Robin Augustine Nandakumar Kalarikkal Sabu Thomas Miran Mozetič Cell Adhesion on Polycaprolactone Modified by Plasma Treatment International Journal of Polymer Science |
| title | Cell Adhesion on Polycaprolactone Modified by Plasma Treatment |
| title_full | Cell Adhesion on Polycaprolactone Modified by Plasma Treatment |
| title_fullStr | Cell Adhesion on Polycaprolactone Modified by Plasma Treatment |
| title_full_unstemmed | Cell Adhesion on Polycaprolactone Modified by Plasma Treatment |
| title_short | Cell Adhesion on Polycaprolactone Modified by Plasma Treatment |
| title_sort | cell adhesion on polycaprolactone modified by plasma treatment |
| url | http://dx.doi.org/10.1155/2016/7354396 |
| work_keys_str_mv | AT ninarecek celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT maticresnik celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT helenamotaln celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT tamaralahturnsek celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT robinaugustine celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT nandakumarkalarikkal celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT sabuthomas celladhesiononpolycaprolactonemodifiedbyplasmatreatment AT miranmozetic celladhesiononpolycaprolactonemodifiedbyplasmatreatment |