Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors
Background. The cytokine network remarkably influences homeostasis. There is not a complex analysis describing physiological values of soluble cytokines in healthy individuals. Methods. Authors used the ELISA method to detect soluble cytokines and costimulatory molecules simultaneously in the plasma...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI/2006/65237 |
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| _version_ | 1832550973110747136 |
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| author | Klabusay Martin Kohutova Viera Coupek Petr Nenickova Marie Tesarova Eva |
| author_facet | Klabusay Martin Kohutova Viera Coupek Petr Nenickova Marie Tesarova Eva |
| author_sort | Klabusay Martin |
| collection | DOAJ |
| description | Background. The cytokine network remarkably influences
homeostasis. There is not a complex analysis describing
physiological values of soluble cytokines in healthy individuals.
Methods. Authors used the ELISA method to detect soluble
cytokines and costimulatory molecules simultaneously in the
plasma of 30 blood donors. Results. Data are shown as (a
range of concentrations; median) IL-2 (0–74.48 pg/mL; 0),
IL-4 (0–65.18 pg/mL; 0), IL-5 (0–86.69 pg/mL; 0),
IL-6 (0–73.17 pg/mL; 0), IL-8 (0–74.8 pg/mL; 0),
IL-10 (0–12.6 pg/mL; 0), IL-13 (0–3.9 pg/mL; 0),
sCD40L (0–3.5 ng/mL; 0), and TNF-α (0–67.6; 0).
For the IL-6 receptor, the concentration was 75.89±35.83
ng/mL (average ±
SD), sCD23 28.9±15.22
ng/mL, and TGFβ1 14.98±12.39
ng/mL. A
special approach was taken for the analysis of values, which had
median 0. A model for the probability of detectable level's
occurrence for each interleukin was formulated with intervals of
confidence. Conclusion. These results provide reference
values for cytokine levels in the plasma of healthy individuals. |
| format | Article |
| id | doaj-art-558833fc8d4f45c48a048996fece4f7e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-558833fc8d4f45c48a048996fece4f7e2025-02-03T06:05:20ZengWileyMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/6523765237Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donorsKlabusay Martin0Kohutova Viera1Coupek Petr2Nenickova Marie3Tesarova Eva4Laboratory of Flow Cytometry and Cellular Therapy, Department of Internal Medicine-Hematooncology, University of Tampere and Research Unit, University Hospital Brno, Jihlavska 20, 625 00 Brno, Czech RepublicLaboratory of Flow Cytometry and Cellular Therapy, Department of Internal Medicine-Hematooncology, University of Tampere and Research Unit, University Hospital Brno, Jihlavska 20, 625 00 Brno, Czech RepublicDivision of Informatics, Czech Geological Survey, Leitnerova 22, 658 69 Brno, Czech RepublicTransfusion Department and Blood Bank, University Hospital Brno, Jihlavska 20, 33014 625 00 Brno, Czech RepublicTransfusion Department and Blood Bank, University Hospital Brno, Jihlavska 20, 33014 625 00 Brno, Czech RepublicBackground. The cytokine network remarkably influences homeostasis. There is not a complex analysis describing physiological values of soluble cytokines in healthy individuals. Methods. Authors used the ELISA method to detect soluble cytokines and costimulatory molecules simultaneously in the plasma of 30 blood donors. Results. Data are shown as (a range of concentrations; median) IL-2 (0–74.48 pg/mL; 0), IL-4 (0–65.18 pg/mL; 0), IL-5 (0–86.69 pg/mL; 0), IL-6 (0–73.17 pg/mL; 0), IL-8 (0–74.8 pg/mL; 0), IL-10 (0–12.6 pg/mL; 0), IL-13 (0–3.9 pg/mL; 0), sCD40L (0–3.5 ng/mL; 0), and TNF-α (0–67.6; 0). For the IL-6 receptor, the concentration was 75.89±35.83 ng/mL (average ± SD), sCD23 28.9±15.22 ng/mL, and TGFβ1 14.98±12.39 ng/mL. A special approach was taken for the analysis of values, which had median 0. A model for the probability of detectable level's occurrence for each interleukin was formulated with intervals of confidence. Conclusion. These results provide reference values for cytokine levels in the plasma of healthy individuals.http://dx.doi.org/10.1155/MI/2006/65237 |
| spellingShingle | Klabusay Martin Kohutova Viera Coupek Petr Nenickova Marie Tesarova Eva Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors Mediators of Inflammation |
| title | Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors |
| title_full | Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors |
| title_fullStr | Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors |
| title_full_unstemmed | Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors |
| title_short | Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5, IL-6, CXCL8 (IL-8), IL-10, IL-13 occurring in plasma of healthy blood donors |
| title_sort | simultaneous analysis of cytokines and co stimulatory molecules concentrations by elisa technique andof probabilities of measurable concentrations of interleukins il 2 il 4 il 5 il 6 cxcl8 il 8 il 10 il 13 occurring in plasma of healthy blood donors |
| url | http://dx.doi.org/10.1155/MI/2006/65237 |
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