Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study

<b>Objective:</b> Accurate risk stratification at an early stage may reduce the incidence of infection and improve the survival rate of recipients by adopting targeted interventions. This study aimed to develop a nomogram to predict the risk of multidrug-resistant organism (MDRO) infecti...

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Main Authors: Chuanlin Chen, Desheng Li, Zhengdon Zhou, Qinghua Guan, Bo Sheng, Yongfang Hu, Zhenyu Zhang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Bioengineering
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Online Access:https://www.mdpi.com/2306-5354/12/4/417
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author Chuanlin Chen
Desheng Li
Zhengdon Zhou
Qinghua Guan
Bo Sheng
Yongfang Hu
Zhenyu Zhang
author_facet Chuanlin Chen
Desheng Li
Zhengdon Zhou
Qinghua Guan
Bo Sheng
Yongfang Hu
Zhenyu Zhang
author_sort Chuanlin Chen
collection DOAJ
description <b>Objective:</b> Accurate risk stratification at an early stage may reduce the incidence of infection and improve the survival rate of recipients by adopting targeted interventions. This study aimed to develop a nomogram to predict the risk of multidrug-resistant organism (MDRO) infections in liver transplant (LT) recipients. <b>Methods:</b> We retrospectively collected clinical data from 301 LT recipients and randomly divided them into a training set (210 cases) and validation set (91 cases) using a 7:3 split ratio. Factors related to the risk of MDRO infection after LT were determined using univariate and multivariate bidirectional stepwise logistic regression. The model’s predictive performance and discrimination ability were evaluated using receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA). <b>Results:</b> 56 (18.60%) patients developed a MDRO infection, including 37 (17.62%) in the training cohort and 19 (20.88%) in the validation cohort. Ultimately, five factors related to MDRO infection after LT surgery were established: ascites (OR = 3.48, 95% CI [1.33–9.14], <i>p</i> = 0.011), total bilirubin (OR = 1.01, 95% CI [1.01–1.01], <i>p</i> < 0.001), albumin (OR = 0.85, 95% CI [0.75–0.96], <i>p</i> = 0.010), history of preoperative ICU stay (OR = 1.09, 95% CI [1.01–1.17], <i>p</i> = 0.009), and length of ICU stay (OR = 3.70, 95% CI [1.39–9.84], <i>p</i> = 0.019). The model demonstrated strong discrimination, and the area under the curve (AUC), sensitivity, and specificity of the training set were 0.88 (95% CI [0.81–0.94]), 0.82 (95% CI [0.76–0.87]), and 0.86 (95% CI [0.75–0.98]), respectively, while for the validation set, they were 0.77 (95% CI [0.65–0.90]), 0.76 (95% CI [0.67–0.86]), and 0.68 (95% CI [0.48–0.89]). The mean absolute error (MAE) in the validation cohort was 0.029, indicating a high accuracy. DCA showed a clinical benefit within a threshold probability range of 0.1 to 0.7. <b>Conclusions:</b> This study developed a clinically accessible nomogram to predict the risk of MDRO infection in LT recipients, enabling early risk stratification and the real-time assessment of infection risk based on the length of postoperative ICU stay. The model incorporates five easily obtainable clinical parameters (ascites, total bilirubin, albumin, preoperative ICU stay history, and length of ICU stay) and demonstrates strong predictive performance, facilitating the early identification of high-risk patients. Future research should focus on refining the model by incorporating additional clinical factors (e.g., immunosuppressive therapy adherence) and validating its generalizability in multicenter, large-sample cohorts to enhance its clinical utility.
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spelling doaj-art-558735855f834c19bd008737d06ad8022025-08-20T03:14:25ZengMDPI AGBioengineering2306-53542025-04-0112441710.3390/bioengineering12040417Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort StudyChuanlin Chen0Desheng Li1Zhengdon Zhou2Qinghua Guan3Bo Sheng4Yongfang Hu5Zhenyu Zhang6School of Clinical Medicine, Qinghai University, Xining 810000, ChinaSchool of Clinical Medicine, Qinghai University, Xining 810000, ChinaSchool of Clinical Medicine, Qinghai University, Xining 810000, ChinaDepartment of Liver ICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing 100000, ChinaDepartment of Liver ICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing 100000, ChinaDepartment of Clinical Pharmacy, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing 100000, ChinaDepartment of Liver ICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing 100000, China<b>Objective:</b> Accurate risk stratification at an early stage may reduce the incidence of infection and improve the survival rate of recipients by adopting targeted interventions. This study aimed to develop a nomogram to predict the risk of multidrug-resistant organism (MDRO) infections in liver transplant (LT) recipients. <b>Methods:</b> We retrospectively collected clinical data from 301 LT recipients and randomly divided them into a training set (210 cases) and validation set (91 cases) using a 7:3 split ratio. Factors related to the risk of MDRO infection after LT were determined using univariate and multivariate bidirectional stepwise logistic regression. The model’s predictive performance and discrimination ability were evaluated using receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA). <b>Results:</b> 56 (18.60%) patients developed a MDRO infection, including 37 (17.62%) in the training cohort and 19 (20.88%) in the validation cohort. Ultimately, five factors related to MDRO infection after LT surgery were established: ascites (OR = 3.48, 95% CI [1.33–9.14], <i>p</i> = 0.011), total bilirubin (OR = 1.01, 95% CI [1.01–1.01], <i>p</i> < 0.001), albumin (OR = 0.85, 95% CI [0.75–0.96], <i>p</i> = 0.010), history of preoperative ICU stay (OR = 1.09, 95% CI [1.01–1.17], <i>p</i> = 0.009), and length of ICU stay (OR = 3.70, 95% CI [1.39–9.84], <i>p</i> = 0.019). The model demonstrated strong discrimination, and the area under the curve (AUC), sensitivity, and specificity of the training set were 0.88 (95% CI [0.81–0.94]), 0.82 (95% CI [0.76–0.87]), and 0.86 (95% CI [0.75–0.98]), respectively, while for the validation set, they were 0.77 (95% CI [0.65–0.90]), 0.76 (95% CI [0.67–0.86]), and 0.68 (95% CI [0.48–0.89]). The mean absolute error (MAE) in the validation cohort was 0.029, indicating a high accuracy. DCA showed a clinical benefit within a threshold probability range of 0.1 to 0.7. <b>Conclusions:</b> This study developed a clinically accessible nomogram to predict the risk of MDRO infection in LT recipients, enabling early risk stratification and the real-time assessment of infection risk based on the length of postoperative ICU stay. The model incorporates five easily obtainable clinical parameters (ascites, total bilirubin, albumin, preoperative ICU stay history, and length of ICU stay) and demonstrates strong predictive performance, facilitating the early identification of high-risk patients. Future research should focus on refining the model by incorporating additional clinical factors (e.g., immunosuppressive therapy adherence) and validating its generalizability in multicenter, large-sample cohorts to enhance its clinical utility.https://www.mdpi.com/2306-5354/12/4/417liver transplantationmulti-resistant organismspostoperative infectionnomogram
spellingShingle Chuanlin Chen
Desheng Li
Zhengdon Zhou
Qinghua Guan
Bo Sheng
Yongfang Hu
Zhenyu Zhang
Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study
Bioengineering
liver transplantation
multi-resistant organisms
postoperative infection
nomogram
title Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study
title_full Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study
title_fullStr Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study
title_full_unstemmed Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study
title_short Epidemiology and Risk Prediction Model of Multidrug-Resistant Organism Infections After Liver Transplant Recipients: A Single-Center Cohort Study
title_sort epidemiology and risk prediction model of multidrug resistant organism infections after liver transplant recipients a single center cohort study
topic liver transplantation
multi-resistant organisms
postoperative infection
nomogram
url https://www.mdpi.com/2306-5354/12/4/417
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